FastQCFastQC Report
Wed 25 May 2016
SRR1294535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609891
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42270.693074664161301No Hit
GGTATCAACGCAGAGTACTTTTTTT32180.527635265973756No Hit
TATCAACGCAGAGTACTTTTTTTTT24750.40581021854724864No Hit
GTGGTATCAACGCAGAGTACATGGG16000.262341959464888No Hit
CCCATGTACTCTGCGTTGATACCAC14140.23184470667709478No Hit
GTACATGGTAAGCAGTGGTATCAAC12440.20397087348395043No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12290.20151141761396707No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11700.19183755785869933No Hit
ACCATGTACTCTGCGTTGATACCAC11540.18921413826405045No Hit
GTGGTAAGCAGTGGTATCAACGCAG10730.17593307656614052No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8980.1472394247496684No Hit
ATTCCATTCCATTCCATTCCATTCC8610.14117276693704284No Hit
CATGTACTCTGCGTTGATACCACTG6810.11165929649724295No Hit
GGTAAGCAGTGGTATCAACGCAGAG6760.11083947787391517No Hit
GAATGGAATGGAATGGAATGGAATG6540.10723227593127296No Hit
GGTATCAACGCAGAGTACATGGGAA6470.10608452985861408No Hit
GTGGTATCAACGCAGAGTACTTTTT6350.10411696516262742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG400.005247252514.2610191
GTCTTAG602.5445943E-514.2610181
GTACTAA500.001489025313.3102831
TTAGACT604.076029E-412.6702214
CTAGGAC701.0859557E-412.2177133
ACTGATC701.0901221E-412.2127058
GCCTAGA550.00304707512.1002591
CAGGACA550.00305833412.0943024
ATTATAC802.8514685E-511.8783333
GTCCTAG657.9593674E-411.7013481
TAAGGTG752.0644878E-411.40325
TAGACTG1001.9165836E-611.4031985
TCGCCCA855.3217933E-511.17410712
CTAGACT600.005856473511.0864434
ACACTAT600.005856473511.0864436
TCTGTCG951.3582794E-510.9985768
CTAGAAC700.001485668610.8601893
TAGACAG700.001485668610.8601895
CCCACAC700.001485668610.8601893
GTATAAG1158.6943146E-710.7474351
Produced by FastQC (version 0.11.5)