FastQCFastQC Report
Wed 25 May 2016
SRR1294534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948098
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG25390.2677993203234265No Hit
GTATCAACGCAGAGTACTTTTTTTT17180.1812048965402311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA15810.166754913521598No Hit
GGTATCAACGCAGAGTACTTTTTTT13500.14239034361426772No Hit
GTACATGGTAAGCAGTGGTATCAAC11300.11918599132157225No Hit
CCCATGTACTCTGCGTTGATACCAC11240.11855314534995327No Hit
ATTCCATTCCATTCCATTCCATTCC11060.11665460743509636No Hit
ACCATGTACTCTGCGTTGATACCAC10730.11317395459119206No Hit
TATCAACGCAGAGTACTTTTTTTTT10190.10747834084662135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT2000.012.3470174
GGTTATC600.00588567211.0800728
GTTCTAA700.001470627810.8745981
GTATAGC700.001470627810.8745981
CCTACAC1408.352799E-910.8550933
GTAATAC803.7743294E-410.6854823
TAGGACT803.7761702E-410.6849184
GTCTTAT909.3515795E-510.5725261
TCTAAAC909.523383E-510.5535633
TATTAGG1451.4280886E-810.4879712
TAGAAGT1002.40206E-510.4474764
CTGATCA1002.403459E-510.4469259
TAGACAG1502.4489054E-810.1303515
GACTGGT750.002652410710.1303517
CCCTACA856.5578363E-410.0638272
TAACCAG1151.0283762E-59.9101265
GTATTAG1553.9519364E-89.8222171
GTGCTAA900.00109643669.5152731
GCAGCAC900.00111459249.4982063
CAGGACT1002.7589762E-49.4977054
Produced by FastQC (version 0.11.5)