##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294534_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	948098
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.545889770888664	33.0	31.0	34.0	30.0	34.0
2	31.648451953279093	33.0	31.0	34.0	30.0	34.0
3	31.758630436937953	34.0	31.0	34.0	30.0	34.0
4	35.046308503973215	37.0	35.0	37.0	33.0	37.0
5	35.295527466569915	37.0	35.0	37.0	33.0	37.0
6	35.27482707483826	37.0	36.0	37.0	33.0	37.0
7	35.28252670082629	37.0	36.0	37.0	33.0	37.0
8	35.33243820786459	37.0	36.0	37.0	33.0	37.0
9	36.921424789420506	39.0	38.0	39.0	34.0	39.0
10	37.03012135876249	39.0	38.0	39.0	34.0	39.0
11	37.07202314528667	39.0	38.0	39.0	35.0	39.0
12	37.09224784779632	39.0	38.0	39.0	34.0	39.0
13	37.02677676780248	39.0	38.0	39.0	34.0	39.0
14	38.488571856495845	41.0	39.0	41.0	34.0	41.0
15	38.553488141521235	41.0	39.0	41.0	35.0	41.0
16	38.57630856725782	41.0	39.0	41.0	35.0	41.0
17	38.50483705270974	41.0	39.0	41.0	34.0	41.0
18	38.5005590139416	41.0	39.0	41.0	34.0	41.0
19	38.55912152541193	41.0	40.0	41.0	34.0	41.0
20	38.57961835168938	41.0	40.0	41.0	34.0	41.0
21	38.566639735554766	41.0	39.0	41.0	34.0	41.0
22	38.56518418982004	41.0	39.0	41.0	34.0	41.0
23	38.562445021506214	41.0	39.0	41.0	34.0	41.0
24	38.641604559866174	41.0	39.0	41.0	34.0	41.0
25	37.96111899824702	41.0	38.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.2797063084721607
1101	2	-0.28309880258839115
1101	3	-0.26154254724806236
1101	4	-0.4250146431598978
1101	5	-0.2734775658509818
1101	6	-0.134938886388845
1101	7	-0.1822926234674327
1101	8	-0.2095825738423116
1101	9	-0.2059209347980655
1101	10	-0.1595953684641529
1101	11	-0.04422655911483275
1101	12	-0.03553626330084114
1101	13	-0.1437675282313009
1101	14	-0.1945295530304776
1101	15	-0.12871156507263493
1101	16	-0.17686777735752912
1101	17	-0.21888397538008775
1101	18	-0.39770504687265174
1101	19	-0.26120602314414043
1101	20	-0.3135184298920848
1101	21	-0.3645909031650163
1101	22	-0.2687588138647854
1101	23	-0.2798157615575718
1101	24	-0.3085271392959257
1101	25	-0.29437573993126875
1102	1	-0.4354437428802669
1102	2	-0.3222212737939607
1102	3	-0.4685241650164542
1102	4	-0.28417853214045863
1102	5	-0.40709031790537153
1102	6	-0.2173338568518659
1102	7	-0.3596869338862305
1102	8	-0.3614603996566359
1102	9	-0.35677321690096875
1102	10	-0.20287611827438923
1102	11	-0.21494287210780527
1102	12	-0.21041085489847688
1102	13	-0.14555459796497416
1102	14	-0.17920745144967043
1102	15	-0.29682482866130044
1102	16	-0.32323918424179254
1102	17	-0.18190994812025707
1102	18	-0.25934162652348647
1102	19	-0.2394328143391533
1102	20	-0.2601555045760833
1102	21	-0.274109565915424
1102	22	-0.3347640612163545
1102	23	-0.33113915022461526
1102	24	-0.26974294075462524
1102	25	-0.308801831479407
1103	1	-0.28779217165356386
1103	2	-0.3019837393077225
1103	3	-0.37950929387723065
1103	4	-0.19590553570684222
1103	5	-0.1217528574383735
1103	6	2.6253639219930847E-4
1103	7	-0.1690284990568145
1103	8	-0.1590076710381041
1103	9	-0.03587792048753613
1103	10	0.022603324562567195
1103	11	0.11777195192826184
1103	12	-0.05816840822598124
1103	13	-0.11022050200911337
1103	14	-0.04084024870422809
1103	15	-0.22868674912425746
1103	16	-0.04080193237449237
1103	17	-0.13889803775083465
1103	18	-9.269841710235482E-4
1103	19	-0.10744640696414365
1103	20	-0.12301466613990897
1103	21	-0.22989820732582444
1103	22	-0.08487677233942748
1103	23	-0.14228577561994626
1103	24	0.005820614860745366
1103	25	0.01880566465140987
1104	1	-0.4559474518778117
1104	2	-0.5645021914813455
1104	3	-0.608342784779687
1104	4	-0.4879922484832093
1104	5	-0.5385132362677183
1104	6	-0.4073347832740879
1104	7	-0.537769272970003
1104	8	-0.5860929739854797
1104	9	-0.5968646349225253
1104	10	-0.48117169990211295
1104	11	-0.5333302026221673
1104	12	-0.5354386475651864
1104	13	-0.30505638132775204
1104	14	-0.4914489631060448
1104	15	-0.5293468808188706
1104	16	-0.5456169130515391
1104	17	-0.5508824135283135
1104	18	-0.6509584979956387
1104	19	-0.6301894366037217
1104	20	-0.6615494391574401
1104	21	-0.5620854326164988
1104	22	-0.6844098701483858
1104	23	-0.508398462422285
1104	24	-0.6679059778313672
1104	25	-0.6062702173210255
1105	1	-0.13614794432126232
1105	2	-0.15102460669721296
1105	3	-0.3185418209831532
1105	4	-0.1293739022027296
1105	5	-0.18468625431673047
1105	6	0.15582669700223306
1105	7	-0.11115288842891147
1105	8	-0.07705842762097603
1105	9	-0.08953573056736275
1105	10	-0.08323487393618478
1105	11	0.04797569314117567
1105	12	0.014058447487116155
1105	13	0.042102862699572086
1105	14	-0.054119171616179074
1105	15	0.0034352573096754213
1105	16	-0.020952057478744734
1105	17	0.12973653868190382
1105	18	-0.11300252331078298
1105	19	-0.07779074152865917
1105	20	-0.12949625794260555
1105	21	-0.13853635816581544
1105	22	-0.051855206749053195
1105	23	-0.05148391692817711
1105	24	-0.1733108041789322
1105	25	-0.18015179990239005
1106	1	-0.029993182131338614
1106	2	-0.2670663068707064
1106	3	-0.20857070244255738
1106	4	-0.060404874488163784
1106	5	-0.09360528699315296
1106	6	-0.020665882447445938
1106	7	-0.1398161969985594
1106	8	-0.20654420760858727
1106	9	-0.09075737523644278
1106	10	-0.23765743295668074
1106	11	-0.19396213643767624
1106	12	-0.024144468719008216
1106	13	-0.17281078378999837
1106	14	-0.1150713700632906
1106	15	-0.03455464102347605
1106	16	-0.18951848996832865
1106	17	-0.1775267719409257
1106	18	-0.04271261368511148
1106	19	-0.08683672943540444
1106	20	-0.1905793468899688
1106	21	-0.13751917643504896
1106	22	-0.2554694955431174
1106	23	-0.25313484821836596
1106	24	-0.32450558635319027
1106	25	-0.39852335098048286
1107	1	-0.20631472167900355
1107	2	-0.06389684226128622
1107	3	0.009626490056241721
1107	4	-0.07515884501795966
1107	5	-0.06226950200126424
1107	6	0.003398353689867406
1107	7	-0.04031986023256451
1107	8	0.06803484328764142
1107	9	0.05036810708796935
1107	10	-0.009892567470899394
1107	11	-0.0749221234247699
1107	12	-0.0994432541251058
1107	13	0.013663674187199604
1107	14	-0.1699125572563034
1107	15	-0.03608692762269783
1107	16	-0.05612193671183263
1107	17	-0.13632524124740542
1107	18	-0.0988504800713983
1107	19	-0.21958179886831886
1107	20	-0.032724481052035514
1107	21	-0.1292976518119744
1107	22	-0.15858286714379233
1107	23	-0.09717071085169948
1107	24	-0.09019611728315624
1107	25	-0.19202310195770877
1108	1	0.08834536911812307
1108	2	0.09364502217469806
1108	3	0.07024286563102677
1108	4	0.09080353636808525
1108	5	0.14339280770728635
1108	6	0.27816021550938785
1108	7	0.2153917939000678
1108	8	0.18270315603857057
1108	9	0.19148426906977534
1108	10	0.20375460571384707
1108	11	0.18309311724942745
1108	12	0.22947723551722277
1108	13	0.11024495870024964
1108	14	0.13214856208946202
1108	15	0.2214977400846223
1108	16	0.20572273610140002
1108	17	0.1822026479839991
1108	18	0.24195712578625717
1108	19	0.24013126256474493
1108	20	0.22374628549908948
1108	21	0.15590168823016626
1108	22	0.20286175808202955
1108	23	0.22497873310798155
1108	24	0.26106336156690446
1108	25	0.2508421515659407
1109	1	0.04163862856931644
1109	2	-0.00839152294322787
1109	3	0.04028840017788937
1109	4	-0.07538776964452154
1109	5	0.07858146170782021
1109	6	0.08016695540553087
1109	7	0.06882885810747297
1109	8	0.07179683798422332
1109	9	0.0345112612877827
1109	10	0.20602023768244493
1109	11	0.04612050106889143
1109	12	0.1698115326252463
1109	13	0.03646202338627802
1109	14	-0.050580508444490135
1109	15	-0.0019326451424248603
1109	16	0.12620762572417732
1109	17	0.07484119331513028
1109	18	0.03303426909716478
1109	19	0.07946371261924412
1109	20	0.09342697802450317
1109	21	0.1251444397960313
1109	22	0.2084647046654169
1109	23	0.17384298011277366
1109	24	0.2080901275262974
1109	25	0.28871479842192826
1110	1	0.23808636157790986
1110	2	0.16573971370985063
1110	3	0.20660606135577098
1110	4	0.20665366979353905
1110	5	0.006703979841987007
1110	6	0.18786757840900492
1110	7	0.24216325037023267
1110	8	0.23835672926747264
1110	9	0.124607182644624
1110	10	0.31708384404308276
1110	11	0.284769660102441
1110	12	0.1401509195051105
1110	13	0.07902701517957667
1110	14	0.11492751294588288
1110	15	0.20664556561982295
1110	16	0.011948258610992468
1110	17	0.17227461312769776
1110	18	0.1647911212529678
1110	19	0.16572013237886551
1110	20	0.198643087870785
1110	21	0.28874197602024054
1110	22	0.3102339992419303
1110	23	0.31525815104162547
1110	24	0.24657691738175203
1110	25	0.19667044692795344
1111	1	0.22106939979257945
1111	2	0.2165038624004474
1111	3	0.20594665747858443
1111	4	0.15054192752005235
1111	5	0.12229051461581975
1111	6	0.35074757233054754
1111	7	0.25576017640845095
1111	8	0.20506452199213498
1111	9	0.09853766392804886
1111	10	0.09985232725134807
1111	11	0.20980927860699694
1111	12	0.015557054403409154
1111	13	0.16590641572808806
1111	14	0.284196216859975
1111	15	0.19464242508448848
1111	16	0.2584459104457508
1111	17	0.25343013475325193
1111	18	0.24053392298677778
1111	19	0.15824960203362792
1111	20	0.3066568801757512
1111	21	0.3164930198502418
1111	22	0.32128195391401704
1111	23	0.3276999896296928
1111	24	0.23402563041352664
1111	25	0.25378712432840445
1112	1	-0.15247992788918197
1112	2	-0.09636044203472949
1112	3	-0.06443624743962317
1112	4	-0.07698752228780847
1112	5	0.038734177590406205
1112	6	0.14094680121088032
1112	7	0.1373587660880915
1112	8	0.08799332525006065
1112	9	0.0890332511649774
1112	10	-0.04504816854717575
1112	11	-0.0031236494154001093
1112	12	-0.06221175116952793
1112	13	0.025258377563446288
1112	14	0.1767107339180356
1112	15	0.21135874509639052
1112	16	0.040591284049277476
1112	17	0.096420377710146
1112	18	0.07264360681300985
1112	19	-0.014484786100283031
1112	20	0.03328972193637014
1112	21	0.005094430721804599
1112	22	0.12869082096339923
1112	23	0.10796946811798591
1112	24	0.16425556077022918
1112	25	0.08539430343964227
1113	1	0.3246512304558067
1113	2	0.13093476876736432
1113	3	0.34982490706448743
1113	4	0.17556822365835956
1113	5	0.1137702833039711
1113	6	0.2173449320758465
1113	7	0.13973841252847308
1113	8	0.11038382820725445
1113	9	0.18900391305817976
1113	10	0.04042635052755372
1113	11	0.11660700692198134
1113	12	-0.03269506458979521
1113	13	0.062057150748699996
1113	14	0.02819471772113502
1113	15	0.09353577435087601
1113	16	0.14405559006132052
1113	17	-0.05238618495506131
1113	18	0.12593408099902348
1113	19	0.16813855087551133
1113	20	0.14834612338371755
1113	21	0.2749766672776488
1113	22	0.4036540774649069
1113	23	0.3280917244026469
1113	24	0.30338905199076294
1113	25	0.2620338004682736
1114	1	0.5605455670221602
1114	2	0.6258577065351965
1114	3	0.6685388372715622
1114	4	0.6610999129224098
1114	5	0.6285090775406772
1114	6	0.7477681901799969
1114	7	0.6853828867653036
1114	8	0.5950473114518502
1114	9	0.6334384721572945
1114	10	0.623835230056045
1114	11	0.6657866851744458
1114	12	0.6073640020529396
1114	13	0.5092681283906089
1114	14	0.6111388155819597
1114	15	0.5736148434785875
1114	16	0.5684702796163137
1114	17	0.4974832517898946
1114	18	0.7728799950363978
1114	19	0.8050949825794476
1114	20	0.6514960545868291
1114	21	0.7513579355026891
1114	22	0.7437142178606706
1114	23	0.741722354383775
1114	24	0.6153270892827223
1114	25	0.7360814454466578
1115	1	0.6919290874794584
1115	2	0.6585753094866185
1115	3	0.7139932819782828
1115	4	0.559903361278522
1115	5	0.46875663307111637
1115	6	0.5566972781210566
1115	7	0.5294250082202225
1115	8	0.5569841802478592
1115	9	0.6264411330079867
1115	10	0.4151401994523809
1115	11	0.47680311684177923
1115	12	0.43529694994526125
1115	13	0.5068516415336148
1115	14	0.6120137600321343
1115	15	0.589924051839489
1115	16	0.577182044433016
1115	17	0.5990022972083224
1115	18	0.6805082472642141
1115	19	0.6011547957547094
1115	20	0.6630953023230006
1115	21	0.6521041040882523
1115	22	0.6471666744099025
1115	23	0.7627248999789629
1115	24	0.7170965937837792
1115	25	0.8473800314610713
1116	1	0.38958741078930714
1116	2	0.37683427811789016
1116	3	0.3604339906863103
1116	4	0.22834442319501136
1116	5	0.2894114529347931
1116	6	0.3466623391211172
1116	7	0.29061312514177473
1116	8	0.38806819613269994
1116	9	0.3611958917761484
1116	10	0.4440257312697824
1116	11	0.32671696261378
1116	12	0.23479147998707361
1116	13	0.2349956742197179
1116	14	0.3358118966188002
1116	15	0.3568816381846389
1116	16	0.4463302392513526
1116	17	0.4183146862340408
1116	18	0.5666856247390584
1116	19	0.4291864222319788
1116	20	0.44615967217999497
1116	21	0.532049022659379
1116	22	0.4871107338252898
1116	23	0.5774002345723943
1116	24	0.5647121979611853
1116	25	0.6920589936009378
1201	1	-0.375828415639905
1201	2	-0.437088557713885
1201	3	-0.43150803090475875
1201	4	-0.2835176037411742
1201	5	-0.2914431799118091
1201	6	-0.16261064289172822
1201	7	-0.28493738224016596
1201	8	-0.20659265163651952
1201	9	-0.13946617484354107
1201	10	-0.18904510378452244
1201	11	-0.12029088095222562
1201	12	-0.11022115394474952
1201	13	-0.11690386982492385
1201	14	-0.1338818520145466
1201	15	-0.0271063717281379
1201	16	-0.13163873472760912
1201	17	-0.21638347617677312
1201	18	-0.31471789575464015
1201	19	-0.2803672800769519
1201	20	-0.28682551939414225
1201	21	-0.4603898525967409
1201	22	-0.29440930634413576
1201	23	-0.29144781000270825
1201	24	-0.15297864729744504
1201	25	-0.20576205809290116
1202	1	-0.5216771128829585
1202	2	-0.5732615314322089
1202	3	-0.6327710417509103
1202	4	-0.5208857511296472
1202	5	-0.5352646823677745
1202	6	-0.22334358807986376
1202	7	-0.378642970537868
1202	8	-0.30255373369898564
1202	9	-0.31583052080575413
1202	10	-0.2651877169808827
1202	11	-0.21922645697521403
1202	12	-0.4624635358052629
1202	13	-0.3134855820221105
1202	14	-0.335121881119278
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2209	25	0.02334254124905044
2210	1	0.08279529457148627
2210	2	0.009817881889873092
2210	3	0.17685782893284596
2210	4	0.010919183198410565
2210	5	0.12724914726832282
2210	6	-2.637247269238415
2210	7	-0.9390232693871567
2210	8	-0.36750588423430486
2210	9	-0.0719321347296713
2210	10	2.900673499794948E-4
2210	11	-0.18431119981931943
2210	12	-0.0797930922667689
2210	13	-0.012604359762100614
2210	14	0.01317788081725979
2210	15	-0.062200215523624536
2210	16	-0.14614491154296871
2210	17	-0.09485114504622771
2210	18	-0.22515342886726586
2210	19	-0.17634730472410354
2210	20	-0.1439420608928046
2210	21	-0.12266383096393696
2210	22	-0.002444104336291275
2210	23	-0.09069508443193541
2210	24	-0.10634272515494558
2210	25	-0.20211737204343905
2211	1	-0.06926885678532457
2211	2	0.07192056788260359
2211	3	0.06131822494887018
2211	4	0.06233600983065912
2211	5	-0.032772001530624095
2211	6	-0.028318570222971573
2211	7	-0.015490943638489796
2211	8	0.07181182883746118
2211	9	-0.02069583500736627
2211	10	-0.018849119373911094
2211	11	-0.1297030003728068
2211	12	-0.02762081529371585
2211	13	-0.024731576456318294
2211	14	0.02067570163357857
2211	15	-0.051632008265400486
2211	16	0.06936277871286478
2211	17	0.10183316149704069
2211	18	0.07092880375999755
2211	19	-0.04532811361802658
2211	20	0.0651781204914883
2211	21	-0.10474451077397617
2211	22	-0.0374668011892183
2211	23	-0.07340022540011404
2211	24	0.027748757690233106
2211	25	0.03392322983994234
2212	1	0.11958472706609058
2212	2	0.02802406207817043
2212	3	0.2579235912588338
2212	4	0.0966189009872025
2212	5	0.2044184977082466
2212	6	0.039326828990063234
2212	7	0.13752237056826289
2212	8	0.09847936489416043
2212	9	-0.008882830771597128
2212	10	0.10414616334922044
2212	11	0.021121958811839647
2212	12	0.17430371329207617
2212	13	0.09622481945387307
2212	14	-0.022311957207740818
2212	15	0.01983787113181279
2212	16	-0.008224262694348283
2212	17	0.05620773986377259
2212	18	0.13140976035654006
2212	19	0.1532208409822573
2212	20	0.05404925933390814
2212	21	0.07571879051732111
2212	22	0.0883116999656508
2212	23	0.16137169764883197
2212	24	0.08369450775230547
2212	25	0.07785986133811207
2213	1	0.2447178751569652
2213	2	0.11065599862491737
2213	3	0.16771116312958156
2213	4	0.07100040466567492
2213	5	0.1611438461337258
2213	6	0.3286956814690356
2213	7	0.2626599021353755
2213	8	0.10912536752911706
2213	9	0.11714552057863159
2213	10	0.06334817585187125
2213	11	0.08664518758303075
2213	12	0.08200182815377133
2213	13	-0.06258608955733536
2213	14	0.0890473344225029
2213	15	0.07580366053500143
2213	16	0.08142367110541926
2213	17	0.11153695110593276
2213	18	0.048229889279440386
2213	19	0.04952191761272928
2213	20	0.16150384773767001
2213	21	0.15954839018417744
2213	22	0.1774443317804355
2213	23	0.22736882890716004
2213	24	0.15465616938202942
2213	25	0.2559717032991813
2214	1	0.346902026881466
2214	2	0.28321376215275507
2214	3	0.3943144602233417
2214	4	0.3897143807089236
2214	5	0.18089397669634621
2214	6	0.36566720093181004
2214	7	0.35273261180464033
2214	8	0.3738238572951502
2214	9	0.34628852897995444
2214	10	0.37923389122773443
2214	11	0.24573222694736074
2214	12	0.2798803941826833
2214	13	0.09216793999870276
2214	14	0.3182776230434001
2214	15	0.2990802935216834
2214	16	0.2647164247009641
2214	17	0.24345265653757764
2214	18	0.350156015873921
2214	19	0.39043702448330464
2214	20	0.28906460407084467
2214	21	0.35555543937349654
2214	22	0.3368304132085882
2214	23	0.3094465750743467
2214	24	0.27133313002723725
2214	25	0.039474787915686704
2215	1	0.4067796266548882
2215	2	0.3557229667975612
2215	3	0.3924230442472805
2215	4	0.4163050295286297
2215	5	0.3330051958961917
2215	6	0.44702271571465246
2215	7	0.36172668881581416
2215	8	0.48071595053605165
2215	9	0.4024522546753815
2215	10	0.27365938593891315
2215	11	0.2195651764972908
2215	12	0.1497347206735924
2215	13	0.32323838785546855
2215	14	0.41774689369074025
2215	15	0.29207860651654727
2215	16	0.2697355728249349
2215	17	0.3981842749542537
2215	18	0.5608075446453569
2215	19	0.3788964316918637
2215	20	0.3594829074608654
2215	21	0.3740903308264123
2215	22	0.4330069453663512
2215	23	0.4695642960166069
2215	24	0.4637329871859279
2215	25	0.4295719446347732
2216	1	0.6336573465701463
2216	2	0.45597604284560944
2216	3	0.5774659889045068
2216	4	0.4330638819722523
2216	5	0.4630607160848754
2216	6	0.5293157412518568
2216	7	0.5154080321539141
2216	8	0.502890108601612
2216	9	0.4775699219925684
2216	10	0.4706762194096541
2216	11	0.3034862376425522
2216	12	0.2524935105004573
2216	13	0.26034925081869886
2216	14	0.3763989636962748
2216	15	0.2588708545623817
2216	16	0.43768570901637815
2216	17	0.47258080598606966
2216	18	0.3294668155776179
2216	19	0.505917648335398
2216	20	0.5256656857662492
2216	21	0.5058370251836024
2216	22	0.2878086067975616
2216	23	0.4625110404671915
2216	24	0.5231855080320855
2216	25	0.46700424026933973
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	4.0
5	7.0
6	9.0
7	72.0
8	226.0
9	473.0
10	763.0
11	1148.0
12	1799.0
13	2815.0
14	3986.0
15	4845.0
16	5273.0
17	5340.0
18	4859.0
19	4380.0
20	3865.0
21	3328.0
22	3085.0
23	2888.0
24	2698.0
25	2645.0
26	2838.0
27	2849.0
28	3069.0
29	3566.0
30	4342.0
31	5839.0
32	7832.0
33	11043.0
34	16428.0
35	26491.0
36	47910.0
37	118880.0
38	642499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15384786046851	21.51522546878681	13.105851951161018	22.225074719583663
2	15.150242489985944	22.115656537943668	40.58794852205625	22.14615245001414
3	17.821192101879387	28.58917979838042	29.37449829803707	24.21512980170312
4	12.035270153808504	18.881177894022873	38.455395955894375	30.62815599627425
5	12.19230229661125	39.86534451278362	34.474061994685925	13.4682911959192
6	29.535740355525924	40.13284754072768	17.493280619069118	12.838131484677278
7	24.245102993005105	35.02405649082203	23.293919578217615	17.436920937955247
8	23.226740138476924	37.794031399916456	21.254541005117993	17.724687456488628
9	23.53864525357717	18.320787040748726	22.328357012350782	35.81221069332332
10	13.901710934368472	31.82359021961562	33.57260395350309	20.702094892512815
11	30.57157684664425	27.337866174987656	22.90431667728502	19.18624030108307
12	20.40841729069906	29.751128954505322	31.116633843213247	18.72381991158237
13	25.900424287561897	25.68564321445827	26.38631899420007	22.027613503779765
14	20.182707167458712	25.378679592600996	28.23564161124092	26.202971628699373
15	22.85424635684462	31.490745214369653	24.51510746438073	21.139900964404994
16	20.82401950287089	32.712669165357724	25.629692298117117	20.833619033654266
17	19.061956051607186	31.14881900562278	28.398615931556126	21.390609011213908
18	19.08890093823118	30.89580296090317	29.558396293491036	20.456899807374622
19	20.15919568726329	29.73847176360126	29.871820569897352	20.2305119792381
20	22.456336922599718	28.937167792400714	28.721410891872555	19.88508439312701
21	22.956144700334427	29.403306290814335	27.21064680500902	20.429902203842218
22	21.280602835233196	29.024950727377853	29.226050551389125	20.468395885999826
23	20.928361716481017	29.532937014432807	28.472250814755224	21.06645045433095
24	20.997291414088657	28.982713376320397	29.02702993583595	20.992965273754997
25	21.748182164477313	28.85459742764798	27.978469820923017	21.41875058695168
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2243.0
1	2243.0
2	2405.0
3	2567.0
4	2567.0
5	2567.0
6	3335.5
7	4104.0
8	4104.0
9	4104.0
10	5580.5
11	7057.0
12	7057.0
13	7057.0
14	9964.0
15	12871.0
16	12871.0
17	12871.0
18	18619.0
19	24367.0
20	24367.0
21	24367.0
22	32749.5
23	41132.0
24	41132.0
25	41132.0
26	51050.0
27	60968.0
28	60968.0
29	60968.0
30	70438.0
31	79908.0
32	79908.0
33	79908.0
34	87495.0
35	95082.0
36	95082.0
37	95082.0
38	100582.5
39	106083.0
40	106083.0
41	106083.0
42	106836.5
43	107590.0
44	107590.0
45	107590.0
46	107866.5
47	108143.0
48	108143.0
49	108143.0
50	103145.5
51	98148.0
52	98148.0
53	98148.0
54	87331.0
55	76514.0
56	76514.0
57	76514.0
58	66391.0
59	56268.0
60	56268.0
61	56268.0
62	45819.0
63	35370.0
64	35370.0
65	35370.0
66	26487.5
67	17605.0
68	17605.0
69	17605.0
70	12500.0
71	7395.0
72	7395.0
73	7395.0
74	5144.5
75	2894.0
76	2894.0
77	2894.0
78	1976.5
79	1059.0
80	1059.0
81	1059.0
82	756.5
83	454.0
84	454.0
85	454.0
86	314.5
87	175.0
88	175.0
89	175.0
90	125.0
91	75.0
92	75.0
93	75.0
94	47.0
95	19.0
96	19.0
97	19.0
98	13.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16443447829232843
2	0.04535396129935935
3	0.007910574645237095
4	0.01065290718891929
5	0.0051682421015549025
6	0.009914586888697159
7	0.012762393760982513
8	0.00738320300222129
9	0.0149773546616489
10	0.00822699763104658
11	0.0056956137445707085
12	0.010231009874506643
13	0.01645399526209316
14	0.014028085704220448
15	0.01645399526209316
16	0.014239034361426773
17	0.017719687205331094
18	0.015188303318855224
19	0.021938660349457545
20	0.02868901738005987
21	0.021938660349457545
22	0.03269704186697999
23	0.09292288349938507
24	0.03913097591177283
25	0.04271710308428031
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	948098.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.81275220362659
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.31666018329081	79.99255528799097
2	4.296160827825924	7.287384474346546
3	0.5913900075840881	1.5047224250679032
4	0.22764936414311535	0.7723027644153279
5	0.12943356258748082	0.54888083352823
6	0.08230068679868263	0.4188088653386971
7	0.048007746457500375	0.2850168372908158
8	0.04025841581769757	0.27315416358855554
9	0.03391935196566961	0.2589114233854738
>10	0.19927577426061904	3.213698924133123
>50	0.018160268952246147	1.0744781183339673
>100	0.014670294379872606	2.4763295667801244
>500	9.945957347562713E-4	0.5609567706045391
>1k	0.001118920201600805	1.332799545195747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	2539	0.2677993203234265	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1718	0.1812048965402311	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1581	0.166754913521598	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1350	0.14239034361426772	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1130	0.11918599132157225	No Hit
CCCATGTACTCTGCGTTGATACCAC	1124	0.11855314534995327	No Hit
ATTCCATTCCATTCCATTCCATTCC	1106	0.11665460743509636	No Hit
ACCATGTACTCTGCGTTGATACCAC	1073	0.11317395459119206	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1019	0.10747834084662135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0547432860316128E-4	0.0
5	0.0	0.0	0.0	1.0547432860316128E-4	0.0
6	0.0	0.0	0.0	1.0547432860316128E-4	0.0
7	0.0	0.0	0.0	1.0547432860316128E-4	0.0
8	0.0	0.0	0.0	1.0547432860316128E-4	0.0
9	0.0	0.0	0.0	2.1094865720632257E-4	0.0
10	0.0	0.0	0.0	3.164229858094838E-4	0.0
11	0.0	0.0	0.0	3.164229858094838E-4	0.0
12	0.0	0.0	0.0	3.164229858094838E-4	0.0
13	0.0	0.0	0.0	3.164229858094838E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACT	200	0.0	12.347017	4
GGTTATC	60	0.005885672	11.080072	8
GTTCTAA	70	0.0014706278	10.874598	1
GTATAGC	70	0.0014706278	10.874598	1
CCTACAC	140	8.352799E-9	10.855093	3
GTAATAC	80	3.7743294E-4	10.685482	3
TAGGACT	80	3.7761702E-4	10.684918	4
GTCTTAT	90	9.3515795E-5	10.572526	1
TCTAAAC	90	9.523383E-5	10.553563	3
TATTAGG	145	1.4280886E-8	10.487971	2
TAGAAGT	100	2.40206E-5	10.447476	4
CTGATCA	100	2.403459E-5	10.446925	9
TAGACAG	150	2.4489054E-8	10.130351	5
GACTGGT	75	0.0026524107	10.130351	7
CCCTACA	85	6.5578363E-4	10.063827	2
TAACCAG	115	1.0283762E-5	9.910126	5
GTATTAG	155	3.9519364E-8	9.822217	1
GTGCTAA	90	0.0010964366	9.515273	1
GCAGCAC	90	0.0011145924	9.498206	3
CAGGACT	100	2.7589762E-4	9.497705	4
>>END_MODULE