FastQCFastQC Report
Wed 25 May 2016
SRR1294534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948098
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78930.8325088756647521No Hit
GGTATCAACGCAGAGTACTTTTTTT62110.6551010549542347No Hit
TATCAACGCAGAGTACTTTTTTTTT45420.47906440051555854No Hit
GTGGTATCAACGCAGAGTACATGGG28310.29859782427554954No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24390.25725188746311034No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17010.17941183295397733No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14350.15135566154553642No Hit
GTACATGGTAAGCAGTGGTATCAAC12860.1356399865836654No Hit
CCCATGTACTCTGCGTTGATACCAC12490.13173743642534844No Hit
ACCATGTACTCTGCGTTGATACCAC11580.12213927252246075No Hit
ATTCCATTCCATTCCATTCCATTCC11480.12108452923642915No Hit
GTGGTATCAACGCAGAGTACTTTTT11250.11865861967855643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC402.7539668E-416.6295263
GTACTAG604.0618933E-412.6768061
CTCCTAT853.903644E-612.3039591
TCTGTCG951.3599969E-510.9989318
CTTACAC1158.7700573E-710.7420543
ACCGTGT909.51454E-510.554538
GTCTTAT1201.5053265E-610.2999051
TTCACCG750.00263997310.1360925
TAATACT1701.546141E-910.0615624
TAGACTG1252.556144E-69.8826895
TAGGTTG900.0011099029.5025865
AGGACTG1304.2156626E-69.5025865
ATTGGAC800.00450258389.5025863
CTAGCAC1201.6921513E-59.5025863
AACTGTG1502.640918E-79.5000797
CCGCGCC800.0045241959.49707219
TGCACTG1554.2322245E-79.1960525
TAGGACT1252.7406097E-59.1224834
CTAGAAC1151.1057839E-49.0894313
GTCTCGC1054.4515877E-49.0548611
Produced by FastQC (version 0.11.5)