FastQCFastQC Report
Wed 25 May 2016
SRR1294533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596364
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG16000.2682925193338297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9680.16231697419696695No Hit
GTATCAACGCAGAGTACTTTTTTTT9420.15795722075779223No Hit
GGTATCAACGCAGAGTACTTTTTTT8150.13666150203566949No Hit
CCCATGTACTCTGCGTTGATACCAC7820.13112796882440927No Hit
ATTCCATTCCATTCCATTCCATTCC7200.12073163370022336No Hit
TATCAACGCAGAGTACTTTTTTTTT6160.10329261994352443No Hit
GTACATGGTAAGCAGTGGTATCAAC6080.10195115734685527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA250.006025351619.0013432
TTAGTAC451.667775E-618.9965613
TAGACAG1101.2732926E-1114.677935
TCTAGAC759.663654E-713.9308123
GTATTGA500.001483658413.316581
GTCTTGG853.8774033E-612.3094431
TCTGTCG802.8647226E-511.8728518
GTACTGA802.8647226E-511.8728516
ACACTGT1603.6379788E-1211.8728516
TAGACTG1052.7181522E-711.758795
GTGTTAA907.3363935E-611.6255861
CTAGACA1001.9270192E-611.3979374
AACAGTC600.00587637311.0813287
CTAGCAC600.00587637311.0813283
TGTACTG600.005880043411.0803995
TCTTGTA951.3551282E-511.0007772
ACAGTCC803.7696297E-410.6855668
TAGGCTG803.772553E-410.68466955
AGACTGT750.002647451410.1314996
GCACTGC750.002647451410.1314996
Produced by FastQC (version 0.11.5)