FastQCFastQC Report
Wed 25 May 2016
SRR1294533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596364
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45440.7619507549080763No Hit
GGTATCAACGCAGAGTACTTTTTTT35100.5885667142885889No Hit
TATCAACGCAGAGTACTTTTTTTTT25880.4339631500224695No Hit
GTGGTATCAACGCAGAGTACATGGG16280.2729876384221717No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14270.23928339068085935No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9810.1644968509165543No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8680.1455486917386026No Hit
CCCATGTACTCTGCGTTGATACCAC7830.1312956516489929No Hit
GTACATGGTAAGCAGTGGTATCAAC7300.1224084619460598No Hit
GTGGTATCAACGCAGAGTACTTTTT6830.11452736919062853No Hit
ATTCCATTCCATTCCATTCCATTCC6610.11083834704978839No Hit
ACCATGTACTCTGCGTTGATACCAC6350.10647859361061365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC607.6503056E-817.4188963
AATACCA701.0874269E-412.2158495
CCTGGAC658.002107E-411.6938043
TCCTATA600.005862875411.0847522
CTAGACA700.001487478610.8585334
ACACCAT1701.364242E-1010.6190066
TGTCCTG909.479302E-510.5569075
ATACTGT1752.3283064E-1010.3156066
GGACAGG750.00262235410.1431371
TATACAG750.00264067510.134635
CTTGCTC1606.1154424E-910.0950423
AGGACAG856.572518E-410.0601115
CTAATAG856.572518E-410.0601113
GACTCTG856.5877405E-410.057587
GTGTAAG951.6240371E-410.0096751
GTCTTGG951.6240371E-410.0096751
TTATACT1054.0869352E-59.9536544
GTTGTAG800.00447273169.5091911
CCAAGAC800.00450380439.5012163
CTACACT800.00450380439.5012164
Produced by FastQC (version 0.11.5)