FastQCFastQC Report
Wed 25 May 2016
SRR1294532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860134
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC83630.44959126600556737No Hit
ACCATGTACTCTGCGTTGATACCAC69320.37266132439920996No Hit
GTACATGGTAAGCAGTGGTATCAAC66240.3561033774986103No Hit
GTGGTAAGCAGTGGTATCAACGCAG48620.2613790189308942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA45170.24283196801950824No Hit
GTGGTATCAACGCAGAGTACATGGG44640.23998271092297652No Hit
CATGTACTCTGCGTTGATACCACTG44480.23912255783723108No Hit
GGTAAGCAGTGGTATCAACGCAGAG36960.1986953628071956No Hit
CCATGTACTCTGCGTTGATACCACT30350.1631602884523373No Hit
GTATCAACGCAGAGTACATGGGAAG29440.15826816777716016No Hit
ACTCTGCGTTGATACCACTGCTTAC28460.15299973012696935No Hit
GGTATCAACGCAGAGTACATGGGAA26940.1448282758123877No Hit
GAGTACATGGTAAGCAGTGGTATCA26200.14085006779081508No Hit
GCAGTGGTATCAACGCAGAGTACAT24720.1328936517476698No Hit
GCTTACCATGTACTCTGCGTTGATA23150.12445339959379272No Hit
GTACATGGGAAGCAGTGGTAAGCAG23000.12364700607590635No Hit
GTATCAACGCAGAGTACTTTTTTTT21920.11784097274712467No Hit
GTACTCTGCGTTGATACCACTGCTT20310.10918568232181122No Hit
GTACATGATAAGCAGTGGTATCAAC20180.10848680793964305No Hit
GCTTACCACTGCTTCCCATGTACTC20100.10805673139677033No Hit
GCGTTGATACCACTGCTTACCATGT19410.10434732121449314No Hit
GTGATAAGCAGTGGTATCAACGCAG19070.1025194959072841No Hit
GTACATAAGCAGTGGTATCAACGCA18820.10117550671080684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTG1355.7105353E-810.5527185
GTCTAAG1502.3932444E-810.1472511
GCTTATG3800.010.0137341
GACCTAT951.6486848E-49.9973126
TATTAGG2151.0913936E-119.7206682
GTCCTAT1502.5956433E-79.5130481
TCAGAAC1401.0619824E-69.4977023
CTAGGAC1701.6709237E-89.4977013
GTCTTAT2357.2759576E-129.3106421
TCTACAC2151.07320375E-109.2768243
TTAAGAC1856.722985E-99.2410073
TATACTG3750.09.1175485
CTTGCAC1151.1135296E-49.0847583
GTATTAG2950.09.0293331
CGCCTCC1901.0622898E-88.99758111
GATAGTC850.00745232738.9387737
GGCTATA1401.0662916E-58.8335441
GTGTAGG1951.6098056E-88.7812751
CCTGTAC1951.6549166E-88.767113
GCTGTTC1304.3834654E-58.7666388
Produced by FastQC (version 0.11.5)