FastQCFastQC Report
Wed 25 May 2016
SRR1294532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860134
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97400.5236181909475339No Hit
CCCATGTACTCTGCGTTGATACCAC85350.4588379116773308No Hit
ACCATGTACTCTGCGTTGATACCAC71410.3838970740817597No Hit
GTACATGGTAAGCAGTGGTATCAAC71130.3823918061817052No Hit
GGTATCAACGCAGAGTACTTTTTTT70070.37669329198864165No Hit
TATCAACGCAGAGTACTTTTTTTTT56820.3054618645753478No Hit
GTGGTAAGCAGTGGTATCAACGCAG55360.2976129676679207No Hit
GTGGTATCAACGCAGAGTACATGGG46720.2511647010376672No Hit
CATGTACTCTGCGTTGATACCACTG46420.2495519140018945No Hit
GGTAAGCAGTGGTATCAACGCAGAG40830.21950031556866334No Hit
CCATGTACTCTGCGTTGATACCACT33210.17853552486003696No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31360.16859000480610536No Hit
GTATCAACGCAGAGTACATGGGAAG31120.16729977517748723No Hit
GGTATCAACGCAGAGTACATGGGAA29430.15821440820930105No Hit
ACTCTGCGTTGATACCACTGCTTAC28480.15310724926268754No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28160.15138694309119666No Hit
GAGTACATGGTAAGCAGTGGTATCA27910.15004295389471942No Hit
GCAGTGGTATCAACGCAGAGTACAT26100.14031247211222417No Hit
GCTTACCATGTACTCTGCGTTGATA24860.13364628569769707No Hit
GTACATGGGAAGCAGTGGTAAGCAG24050.12929176070111079No Hit
GTGATAAGCAGTGGTATCAACGCAG21090.11337892861482023No Hit
GTACTCTGCGTTGATACCACTGCTT21000.1128950925040884No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20770.11165862244332934No Hit
GTACATGATAAGCAGTGGTATCAAC20720.1113898246040339No Hit
GCTTACCACTGCTTCCCATGTACTC20690.11122854590045664No Hit
TATCAACGCAGAGTACATGGGAAGC20010.10757289528603854No Hit
GCGTTGATACCACTGCTTACCATGT19540.10504619559666131No Hit
GTACATAAGCAGTGGTATCAACGCA19360.1040785233751977No Hit
GTATCAACGCAGAGTACATGGTAAG18920.10171310238939776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACGC400.00527962114.257
CTTGGAC1102.737579E-912.9576813
TAATACT3400.010.6202164
TATACTG3250.010.525345
TCTGTCG1606.182745E-910.0926648
TTACACC1651.0614349E-79.2143514
CTAATAC2401.0913936E-119.106373
GTCTTAC2304.1836756E-119.0945371
GTTAGCC2304.1836756E-119.0891553
CTGTCGC2002.6593625E-99.024039
GCTAGAC850.00742116438.943343
GACCCTA850.00743591138.9411767
TCAGTAC1304.3558823E-58.7713543
CAGGACA2401.0186341E-108.7104414
GGCGCGA1201.7672803E-48.70669515
TATTATA5250.08.6882842
CAATACT2002.5263944E-88.552074
GGTATAG1451.6711949E-58.5243451
AGGCCGA1901.0142321E-78.49817316
GTGTTAG3150.08.45148851
Produced by FastQC (version 0.11.5)