FastQCFastQC Report
Wed 25 May 2016
SRR1294531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882074
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC376351.9996556989788923No Hit
ACCATGTACTCTGCGTTGATACCAC277401.473905914432695No Hit
GTACATGGTAAGCAGTGGTATCAAC252631.3422957864568554No Hit
GTGGTAAGCAGTGGTATCAACGCAG220901.1737051784361294No Hit
CATGTACTCTGCGTTGATACCACTG193861.0300338881467996No Hit
GGTAAGCAGTGGTATCAACGCAGAG161090.8559174612687916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA148560.7893419706132703No Hit
CCATGTACTCTGCGTTGATACCACT126640.6728747116213284No Hit
ACTCTGCGTTGATACCACTGCTTAC123090.6540125414834911No Hit
GTATCAACGCAGAGTACATGGGAAG116480.6188917120155744No Hit
GTACTCTGCGTTGATACCACTGCTT104940.5575763758491961No Hit
GTACATGGGAAGCAGTGGTAAGCAG104760.5566199841238973No Hit
GCAGTGGTATCAACGCAGAGTACAT97620.5186831123537119No Hit
GAGTACATGGTAAGCAGTGGTATCA94200.5005116695730348No Hit
GCTTACCATGTACTCTGCGTTGATA92270.4902570249628867No Hit
GCTTACCACTGCTTCCCATGTACTC90910.483030954149518No Hit
GTACATGGGAAGCAGTGGTATCAAC84800.45056676836298676No Hit
GCGTTGATACCACTGCTTACCATGT83910.44583794261012055No Hit
GCGTTGATACCACTGCTTACCACTG82390.43776174581870847No Hit
GGTATCAACGCAGAGTACATGGGAA80590.4281978285657206No Hit
CTGCTTACCATGTACTCTGCGTTGA75960.4035973080760905No Hit
GTACATGATAAGCAGTGGTATCAAC75820.4028534478453026No Hit
TATCAACGCAGAGTACATGGGAAGC73900.3926519361087821No Hit
GTGGTATCAACGCAGAGTACATGGG73490.39047348829004597No Hit
ATCATGTACTCTGCGTTGATACCAC70450.3743210947072219No Hit
GTATCAACGCAGAGTACATGGTAAG70030.37208951401485807No Hit
AAGCAGTGGTATCAACGCAGAGTAC69970.3717707167730918No Hit
GTACATAAGCAGTGGTATCAACGCA65000.34536367858011957No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA64400.34217570616245696No Hit
CAGTGGTATCAACGCAGAGTACATG59490.316087465211251No Hit
TATCAACGCAGAGTACATGGTAAGC56390.29961627438666066No Hit
ACGCAGAGTACATGGTAAGCAGTGG55050.2924964693205474No Hit
ATGGTAAGCAGTGGTATCAACGCAG52530.27910698516636434No Hit
ACGCAGAGTACATGGGAAGCAGTGG49920.2652393051495318No Hit
GTACATGGTAAGCAGTGGTAAGCAG48420.2572693741053752No Hit
TACCACTGCTTACCATGTACTCTGC44760.23782274235763312No Hit
GGTATCAACGCAGAGTACATGGTAA43960.23357211246741627No Hit
GAGTACATGGGAAGCAGTGGTAAGC43390.23054353867063676No Hit
GCTTACCACTGCTTACCATGTACTC42610.2263991745276753No Hit
GCTTATGTACTCTGCGTTGATACCA40930.21747285175821993No Hit
GTGGTATCAACGCAGAGTACATGGT38620.20519915795021876No Hit
GTGATAAGCAGTGGTATCAACGCAG36830.19568837357085853No Hit
GCTTCCCATGTACTCTGCGTTGATA36580.19436005173016577No Hit
GAGTACATGGGAAGCAGTGGTATCA36450.19366932437300552No Hit
GTAAGCAGTGGTATCAACGCAGAGT31470.1672091533064056No Hit
AAAAAGTACTCTGCGTTGATACCAC31430.16699662181189476No Hit
GCAGAGTACATGGTAAGCAGTGGTA28880.1534477390368285No Hit
GAGTACATGATAAGCAGTGGTATCA28680.1523850815642743No Hit
CTGCTTACCACTGCTTCCCATGTAC28580.1518537528279972No Hit
TGGTAAGCAGTGGTATCAACGCAGA27640.1468592627069924No Hit
CTTCCCATGTACTCTGCGTTGATAC27330.14521214362453336No Hit
ACTCTGCGTTGATACCACTGCTTAT27290.14499961213002252No Hit
GCGTTGATACCACTGCTTCCCATGT27030.14361815741570205No Hit
GAGTAAGCAGTGGTATCAACGCAGA26500.14080211511343338No Hit
GATAAGCAGTGGTATCAACGCAGAG26300.13973945764087914No Hit
AAAGTACTCTGCGTTGATACCACTG25570.13586075786605628No Hit
GCTTATCATGTACTCTGCGTTGATA25290.1343730374044804No Hit
CTGCTTCCCATGTACTCTGCGTTGA23010.12225874221736234No Hit
GTACATGGGAAGTAAGCAGTGGTAT22580.11997402865137076No Hit
GTACATGGGTAAGCAGTGGTATCAA22270.11832690956891175No Hit
GCGTTGATACCACTGCTTATCATGT21930.11652039186556959No Hit
GTATCAACGCAGAGTACTTTTTTTT21610.11482013990948284No Hit
GTTGATACCACTGCTTACCATGTAC21410.11375748243692863No Hit
CTTATGTACTCTGCGTTGATACCAC21380.11359808381604548No Hit
CCACTGCTTACCATGTACTCTGCGT21250.11290735645888524No Hit
GATACCACTGCTTACCATGTACTCT21220.11274795783800212No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT20930.11120710450279851No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT20560.1092411881785732No Hit
ATACCACTGCTTACCATGTACTCTG19900.10573441851914431No Hit
GTTGATACCACTGCTTACCACTGCT19450.10334343920589731No Hit
GCAGTGGTAAGCAGTGGTATCAACG19330.10270584472236481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC500.001502901313.2962983
GAACAAA6450.013.1293011
GGTAAAT905.304137E-712.686741
GTCCTAG701.0751243E-412.2336431
CCTATAC701.09397515E-412.2108863
TAGACAT701.09424655E-412.2105615
TCCTATA550.003064874312.0930072
TTGGACT1052.730685E-711.7586314
ACCATAC1001.8936789E-611.4180671
CCGGAAC752.0791146E-411.3971313
GGGTAGA600.00580757511.1008991
GTCCATA600.00580757511.1008991
CGTAGAT600.00586885211.0852572
CACAGCG951.3651112E-510.9966479
GTATAGA1303.1915988E-810.97890951
GCTAGGC700.00148949810.8590262
CTAACCT803.7811825E-410.68452554
ACCGGTG909.548639E-510.5520567
TTTTTAC2900.010.4993721
CATACTC1451.44973455E-810.4798413
Produced by FastQC (version 0.11.5)