FastQCFastQC Report
Wed 25 May 2016
SRR1294531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882074
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC388272.0629900843431237No Hit
ACCATGTACTCTGCGTTGATACCAC289971.5406939365827272No Hit
GTACATGGTAAGCAGTGGTATCAAC260681.3850677497271626No Hit
GTGGTAAGCAGTGGTATCAACGCAG246811.311372454005528No Hit
CATGTACTCTGCGTTGATACCACTG207391.1019226661650923No Hit
GGTAAGCAGTGGTATCAACGCAGAG179730.9549571377108445No Hit
CCATGTACTCTGCGTTGATACCACT135400.7194191089192029No Hit
ACTCTGCGTTGATACCACTGCTTAC125050.6644265847145223No Hit
GTATCAACGCAGAGTACATGGGAAG124550.6617699410331368No Hit
GTACATGGGAAGCAGTGGTAAGCAG107550.5714440558660286No Hit
GCAGTGGTATCAACGCAGAGTACAT107070.5688936779318986No Hit
GTACTCTGCGTTGATACCACTGCTT103120.5479061928489528No Hit
GAGTACATGGTAAGCAGTGGTATCA97850.5199051684471493No Hit
GCTTACCATGTACTCTGCGTTGATA93840.49859888612243725No Hit
GCTTACCACTGCTTCCCATGTACTC88840.4720324493085819No Hit
GGTATCAACGCAGAGTACATGGGAA87540.4651251757369795No Hit
GTACATGGGAAGCAGTGGTATCAAC85540.45449860101143735No Hit
GTATCAACGCAGAGTACTTTTTTTT83900.4457848097364928No Hit
GCGTTGATACCACTGCTTACCATGT83490.44360636191775665No Hit
GCGTTGATACCACTGCTTACCACTG83150.44179984421441454No Hit
AAGCAGTGGTATCAACGCAGAGTAC82010.4357426966208555No Hit
GTGGTATCAACGCAGAGTACATGGG81760.4344143747801627No Hit
CTGCTTACCATGTACTCTGCGTTGA79000.41974970165891456No Hit
GTACATGATAAGCAGTGGTATCAAC77370.41108904325759776No Hit
TATCAACGCAGAGTACATGGGAAGC76950.4088574625652339No Hit
GTATCAACGCAGAGTACATGGTAAG73980.3930769990978038No Hit
ATCATGTACTCTGCGTTGATACCAC73680.39148301288897247No Hit
CAGTGGTATCAACGCAGAGTACATG66200.3517396234154449No Hit
GTACATAAGCAGTGGTATCAACGCA66080.35110202893191234No Hit
ACGCAGAGTACATGGTAAGCAGTGG59320.3151842063595799No Hit
ATGGTAAGCAGTGGTATCAACGCAG58430.3104553806067137No Hit
TATCAACGCAGAGTACATGGTAAGC57520.305620289106592No Hit
ACGCAGAGTACATGGGAAGCAGTGG55550.295153113001933No Hit
TATCAACGCAGAGTACTTTTTTTTT54060.2872363148314041No Hit
GGTATCAACGCAGAGTACTTTTTTT52590.2794257824081306No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49630.2636984518143282No Hit
GTACATGGTAAGCAGTGGTAAGCAG49610.2635921860670728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA49330.26210446560549694No Hit
GAGTACATGGGAAGCAGTGGTAAGC46030.2445706173083524No Hit
GGTATCAACGCAGAGTACATGGTAA45050.23936359569283672No Hit
TACCACTGCTTACCATGTACTCTGC43790.23266885361574516No Hit
GCTTATGTACTCTGCGTTGATACCA42440.22549591567600424No Hit
GTGGTATCAACGCAGAGTACATGGT42260.22453952395070542No Hit
GCTTACCACTGCTTACCATGTACTC42140.22390192946717294No Hit
GTGATAAGCAGTGGTATCAACGCAG40630.21587886554938862No Hit
GCTTCCCATGTACTCTGCGTTGATA39010.20727134002169947No Hit
GAGTACATGGGAAGCAGTGGTATCA38290.2034457731205043No Hit
GTAAGCAGTGGTATCAACGCAGAGT34190.1816612949331429No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31340.16651842594924535No Hit
GCAGAGTACATGGTAAGCAGTGGTA31340.16651842594924535No Hit
GAGTACTTTTTTTTTTTTTTTTTTT31080.16513697123492488No Hit
AAAAAGTACTCTGCGTTGATACCAC30950.16444624387776463No Hit
TGGTAAGCAGTGGTATCAACGCAGA30760.16343671927883813No Hit
GAGTACATGATAAGCAGTGGTATCA30690.16306478916344416No Hit
GATAAGCAGTGGTATCAACGCAGAG29400.15621064846546948No Hit
GCGTTGATACCACTGCTTCCCATGT27900.14824071742131287No Hit
ACTCTGCGTTGATACCACTGCTTAT27870.14808131880042974No Hit
CTGCTTACCACTGCTTCCCATGTAC27480.14600913672894902No Hit
AAAGTACTCTGCGTTGATACCACTG27460.1459028709816936No Hit
GAGTAAGCAGTGGTATCAACGCAGA27150.14425575189923456No Hit
CTTCCCATGTACTCTGCGTTGATAC26700.14186477258598756No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT25430.13511689763526832No Hit
GCTTATCATGTACTCTGCGTTGATA25090.13331037993192615No Hit
GTACATGGGAAGTAAGCAGTGGTAT23900.1269875679702286No Hit
GTACATGGGTAAGCAGTGGTATCAA23580.12528731601414184No Hit
CTGCTTCCCATGTACTCTGCGTTGA23100.12273693808001174No Hit
GTGGTATCAACGCAGAGTACTTTTT23020.12231187509099005No Hit
GCGTTGATACCACTGCTTATCATGT22870.12151488198657437No Hit
GCAGTGGTAAGCAGTGGTATCAACG22840.12135548336569126No Hit
CTTATGTACTCTGCGTTGATACCAC22810.12119608474480813No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT22810.12119608474480813No Hit
GATACCACTGCTTACCATGTACTCT21290.1131198879533961No Hit
CCACTGCTTACCATGTACTCTGCGT21110.1121634962280973No Hit
GTTGATACCACTGCTTACCATGTAC20920.11115397162917079No Hit
CATGGTAAGCAGTGGTATCAACGCA20800.11051637714563826No Hit
ATACCACTGCTTACCATGTACTCTG20760.11030384565112743No Hit
GTTGATACCACTGCTTACCACTGCT20070.10663767737081539No Hit
GAGTACATGGTAAGCAGTGGTAAGC19400.10307777483775876No Hit
AAGCAGTGGTAAGCAGTGGTATCAA19070.10132439000804432No Hit
ATCAACGCAGAGTACATGGGAAGCA19000.10095245989265034No Hit
TACCACTGCTTACCACTGCTTCCCA18980.10084619414539493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA250.006035368418.99845916
ACACCGT350.002169293416.286996
GGACTAT400.005276907714.2511176
GACTAAT551.9534616E-413.8240391
GACAGTA551.9600785E-413.818537
CTTAAGG500.00149886813.3010423
TCTAGGC751.47778555E-512.6676583
AATCGAA604.0918702E-412.66698557
TGTACCG701.0906094E-412.2149185
CTATACC550.00306705912.0918574
CCGGCTC550.00307011212.09025115
GTGTAGA1501.4551915E-1112.0384341
CCCTTAG657.996324E-411.6972641
CCTATAC658.019535E-411.6932233
TGATCTG658.0213224E-411.6929125
TAAGACT752.0727506E-411.4008924
GGAATCG600.00587417711.0839075
TATCCCG600.00587417711.0839075
GTATTAG1553.110472E-1011.0369361
ACAGTCC1158.8245724E-710.7385458
Produced by FastQC (version 0.11.5)