FastQCFastQC Report
Wed 25 May 2016
SRR1294530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841497
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC78120.42422007746958046No Hit
ACCATGTACTCTGCGTTGATACCAC57050.30980229671837645No Hit
GTACATGGTAAGCAGTGGTATCAAC51550.27993529177620163No Hit
GTGGTAAGCAGTGGTATCAACGCAG50390.2736360689156702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41410.22487139539190126No Hit
CATGTACTCTGCGTTGATACCACTG38640.20982928563011505No Hit
GTGGTATCAACGCAGAGTACATGGG37470.20347575912423424No Hit
GGTAAGCAGTGGTATCAACGCAGAG35170.19098592069387027No Hit
GTATCAACGCAGAGTACATGGGAAG26810.14558807318176462No Hit
CCATGTACTCTGCGTTGATACCACT26600.14444769662942702No Hit
GTACATGGGAAGCAGTGGTAAGCAG25700.13956036854798026No Hit
ACTCTGCGTTGATACCACTGCTTAC25150.13657366805376278No Hit
GGTATCAACGCAGAGTACATGGGAA22840.12402952597804938No Hit
GCTTACCACTGCTTCCCATGTACTC21360.1159925864663369No Hit
GCAGTGGTATCAACGCAGAGTACAT21060.11436347710585464No Hit
GCTTACCATGTACTCTGCGTTGATA20450.11105095473954071No Hit
GAGTACATGGTAAGCAGTGGTATCA19720.10708678862903387No Hit
GTACTCTGCGTTGATACCACTGCTT19380.10524046468715399No Hit
ATTCCATTCCATTCCATTCCATTCC19200.10426299907086461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC508.727458E-515.1975233
CTGTCGC1052.7312853E-711.7583919
ACTGCCG600.005890631611.07972110
GTCTCGC700.001475144810.87161351
GGAACGA803.7770704E-410.68575815
ATAGGAC1106.0577804E-610.3619483
GTATAAA3750.010.1468391
ATTTTCG750.002651551310.13168115
TAGGACT1803.947207E-1010.0261444
GAATTAG1054.045677E-59.9656461
CTTAGAC1054.112685E-59.9507593
GTCTTAG1502.5970985E-79.5126621
GTCTAAG1201.6737798E-59.5126611
TCCTAGA2201.6370905E-119.52
ATAACTC1606.650589E-89.4984513
TAGACTG1901.0550139E-99.4979365
GTCCTAG1752.611523E-89.2408711
TTAGGGT1752.6759153E-89.2270674
GTATTAT2900.09.1846391
CTATCCT1356.8606514E-69.1466584
Produced by FastQC (version 0.11.5)