FastQCFastQC Report
Wed 25 May 2016
SRR1294530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC81500.44257470959768064No Hit
ACCATGTACTCTGCGTTGATACCAC61360.33320716786397153No Hit
GTATCAACGCAGAGTACTTTTTTTT61270.3327184350558269No Hit
GTGGTAAGCAGTGGTATCAACGCAG57840.3140922847009797No Hit
GTACATGGTAAGCAGTGGTATCAAC55360.30062498065432636No Hit
GGTATCAACGCAGAGTACTTTTTTT43210.23464605155479484No Hit
CATGTACTCTGCGTTGATACCACTG39870.2165086340080923No Hit
GTGGTATCAACGCAGAGTACATGGG38770.21053523301965738No Hit
GGTAAGCAGTGGTATCAACGCAGAG38010.2064081559731023No Hit
TATCAACGCAGAGTACTTTTTTTTT36540.19842552010673925No Hit
GTATCAACGCAGAGTACATGGGAAG29130.15818651890282745No Hit
CCATGTACTCTGCGTTGATACCACT28440.15443956737371822No Hit
ACTCTGCGTTGATACCACTGCTTAC26660.14477351850152348No Hit
GTACATGGGAAGCAGTGGTAAGCAG26450.14363314194918592No Hit
GGTATCAACGCAGAGTACATGGGAA24440.13271810923395477No Hit
GCAGTGGTATCAACGCAGAGTACAT22850.12408382962339878No Hit
GCTTACCATGTACTCTGCGTTGATA21840.11859916144310852No Hit
GCTTACCACTGCTTCCCATGTACTC21500.11675283750122861No Hit
GAGTACATGGTAAGCAGTGGTATCA21370.1160468901116863No Hit
GTACTCTGCGTTGATACCACTGCTT19590.10638084123949156No Hit
ATTCCATTCCATTCCATTCCATTCC19430.10551198291390103No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18940.10285110429177999No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18880.10252528241968355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG707.255603E-613.5735
CCCCGCG701.0918686E-412.21337812
ATTGGAC658.0168666E-411.6936623
CCATGCG658.029662E-411.6914399
CCAGGAC2800.010.85843
TATCCCG700.001490188510.85845
CTGTCGC1408.361894E-910.8563369
AGACGCC700.001492553710.8563368
TTAGACA1855.456968E-1210.7850324
GTCTAAG1105.9947524E-610.3710341
TTGGACC1201.5215392E-610.2928584
GTACTAA1855.820766E-1110.27761
TACATCG750.002645427110.1345075
CAGGACC1901.0004442E-1010.0011584
TCTGTCG1356.449136E-79.8511198
GGTTTAC1201.6860795E-59.5067811
CTCCTAG1502.6205453E-79.5067811
CTAGATT1606.622031E-89.5011014
ATAATAC2900.09.5011013
ACTAGAC800.00451180669.5011013
Produced by FastQC (version 0.11.5)