FastQCFastQC Report
Wed 25 May 2016
SRR1294529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342168
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC148060.632149359055371No Hit
ACCATGTACTCTGCGTTGATACCAC116690.49821362088458215No Hit
GTACATGGTAAGCAGTGGTATCAAC109370.46696052546187977No Hit
GTGGTAAGCAGTGGTATCAACGCAG91840.39211533929248454No Hit
CATGTACTCTGCGTTGATACCACTG80710.34459526387517886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA66490.28388228342288No Hit
GGTAAGCAGTGGTATCAACGCAGAG64150.27389153980414727No Hit
GTGGTATCAACGCAGAGTACATGGG56420.24088792947388912No Hit
CCATGTACTCTGCGTTGATACCACT52700.2250052088492371No Hit
ACTCTGCGTTGATACCACTGCTTAC50130.21403246906285117No Hit
GTATCAACGCAGAGTACATGGGAAG48460.20690232297597783No Hit
GTACATGGGAAGCAGTGGTAAGCAG43820.18709161768071292No Hit
GCAGTGGTATCAACGCAGAGTACAT42620.18196815941469613No Hit
GCTTACCATGTACTCTGCGTTGATA41310.1763750508076278No Hit
GCTTACCACTGCTTCCCATGTACTC40920.17470992687117234No Hit
GAGTACATGGTAAGCAGTGGTATCA40620.17342906230466817No Hit
GTACTCTGCGTTGATACCACTGCTT39610.16911681826410405No Hit
GTACATGATAAGCAGTGGTATCAAC38160.16292597285933375No Hit
GGTATCAACGCAGAGTACATGGGAA38080.16258440897493262No Hit
GTACATAAGCAGTGGTATCAACGCA35960.15353296603830296No Hit
GCGTTGATACCACTGCTTACCATGT35050.14964767685324024No Hit
ATCATGTACTCTGCGTTGATACCAC33240.14191979396866494No Hit
GCGTTGATACCACTGCTTACCACTG33180.1416636210553641No Hit
CTGCTTACCATGTACTCTGCGTTGA30490.13017853544237645No Hit
GTATCAACGCAGAGTACATGGTAAG28820.12304838935550311No Hit
TATCAACGCAGAGTACATGGGAAGC28200.12040126925139444No Hit
AAGCAGTGGTATCAACGCAGAGTAC28070.11984622793924263No Hit
GTGATAAGCAGTGGTATCAACGCAG25500.10887348815285668No Hit
GTACATGGGAAGCAGTGGTATCAAC25230.10772071004300288No Hit
GAGTAAGCAGTGGTATCAACGCAGA24370.10404889828569087No Hit
CAGTGGTATCAACGCAGAGTACATG24070.10276803371918666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA802.8745499E-511.8722786
AGTACGG1001.9346062E-611.3981163
TAGTACG1408.35098E-910.8576682
ATTAGAC1408.378265E-910.855353
CCGTGCA700.001494747110.8546539
TAAGGCT1502.1391315E-910.7651174
TCTGTCG1158.845527E-710.7368988
GTCTAAG1951.8189894E-1210.7310731
GTACGGG909.5327705E-510.5540374
GCCCTAG1409.5222276E-810.1910361
TAATACG750.002651591310.1318754
GTCTAGG1151.01202295E-59.9251821
GTCCAAC1451.5893056E-79.839621
TAGACAG3050.09.6535245
TTATACT3350.09.6404044
CTGTCGC1502.6581802E-79.4978229
TGTCGCC1304.2560223E-69.49721310
TTAGACT1554.2707507E-79.1922264
GTGTTAC1356.743894E-69.159351
GTCCTAT1356.743894E-69.159351
Produced by FastQC (version 0.11.5)