FastQCFastQC Report
Wed 25 May 2016
SRR1294529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342168
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC154290.658748646553108No Hit
ACCATGTACTCTGCGTTGATACCAC121210.5175119803532454No Hit
GTACATGGTAAGCAGTGGTATCAAC115860.4946698955839206No Hit
GTGGTAAGCAGTGGTATCAACGCAG101370.4328041370217679No Hit
CATGTACTCTGCGTTGATACCACTG84870.3623565858640371No Hit
GTATCAACGCAGAGTACTTTTTTTT78960.3371235539039044No Hit
GGTAAGCAGTGGTATCAACGCAGAG70740.3020278647816894No Hit
GTGGTATCAACGCAGAGTACATGGG59950.25595943587308856No Hit
CCATGTACTCTGCGTTGATACCACT55980.239009328109683No Hit
GGTATCAACGCAGAGTACTTTTTTT54490.23264770076271216No Hit
GTATCAACGCAGAGTACATGGGAAG51060.21800314921901418No Hit
ACTCTGCGTTGATACCACTGCTTAC51010.21778967179126346No Hit
GTACATGGGAAGCAGTGGTAAGCAG47590.20318781573311565No Hit
TATCAACGCAGAGTACTTTTTTTTT46580.19887557169255152No Hit
GAGTACATGGTAAGCAGTGGTATCA44110.18832978676166695No Hit
GCAGTGGTATCAACGCAGAGTACAT43900.18743318156511402No Hit
GCTTACCATGTACTCTGCGTTGATA43020.18367597883670173No Hit
GCTTACCACTGCTTCCCATGTACTC42210.1802176445071404No Hit
GGTATCAACGCAGAGTACATGGGAA40140.17137967899826143No Hit
GTACTCTGCGTTGATACCACTGCTT39980.1706965512294592No Hit
GTACATGATAAGCAGTGGTATCAAC38310.16356640514258583No Hit
GTACATAAGCAGTGGTATCAACGCA36570.15613739065686152No Hit
GCGTTGATACCACTGCTTACCATGT36360.1552407854603086No Hit
ATCATGTACTCTGCGTTGATACCAC34780.14849489874338648No Hit
GCGTTGATACCACTGCTTACCACTG34080.14550621475487668No Hit
AAGCAGTGGTATCAACGCAGAGTAC31300.1336368697719378No Hit
CTGCTTACCATGTACTCTGCGTTGA30880.1318436593788319No Hit
TATCAACGCAGAGTACATGGGAAGC29990.12804376116486949No Hit
GTGATAAGCAGTGGTATCAACGCAG29420.1256101184885115No Hit
GTACATGGGAAGCAGTGGTATCAAC28320.12091361507799613No Hit
GTATCAACGCAGAGTACATGGTAAG28190.12035857376584429No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28180.12031587828029416No Hit
CAGTGGTATCAACGCAGAGTACATG27370.11685754395073283No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26590.11352729607782193No Hit
ATGGTAAGCAGTGGTATCAACGCAG24580.10494550348224381No Hit
GAGTAAGCAGTGGTATCAACGCAGA24530.1047320260544931No Hit
ACGCAGAGTACATGGTAAGCAGTGG24220.10340846600243876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG400.005273431514.2527395
TTAGCAC3350.010.2111363
GTGTTAC1606.0936145E-910.1019471
GACAGTC1701.5588739E-910.0592537
GTACTAA1252.5445388E-69.8880231
TAGCACT3850.09.8722374
GCACCGT1356.4288724E-79.8537456
GTAGGAC1554.0521627E-89.8085463
TTAGAAC1951.6370905E-109.745673
GGCTTAG800.0044867469.5077151
TGAACTG3300.09.5018265
TAAGCTG1804.1709427E-99.5018265
ACCGTGC900.00111482769.4991888
TCAAGAC3150.09.3512033
GTCCAAG2151.05501385E-109.2866051
CTAGACA2151.05501385E-109.2810514
ACATAAG7500.09.2486423
TAGACAG2700.09.1499065
TATTAGG2501.8189894E-129.1225332
ATTAGGG1151.1083457E-49.0888983
Produced by FastQC (version 0.11.5)