FastQCFastQC Report
Wed 25 May 2016
SRR1294528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052534
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC22880.21738015114001066No Hit
GTACATGGTAAGCAGTGGTATCAAC22340.21224967554492302No Hit
ACCATGTACTCTGCGTTGATACCAC21550.20474397976692438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA19980.1898275970182436No Hit
GTGGTATCAACGCAGAGTACATGGG16330.15514938234774364No Hit
CATGTACTCTGCGTTGATACCACTG13670.12987703960157104No Hit
GTGGTAAGCAGTGGTATCAACGCAG13440.12769183703329298No Hit
ATTCCATTCCATTCCATTCCATTCC12930.12284638786015463No Hit
GGTAAGCAGTGGTATCAACGCAGAG11060.10507974089198069No Hit
GAATGGAATGGAATGGAATGGAATG10880.1033695823602848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTCG400.005284963614.2470079
TGTCGCC1001.4455327E-712.34681910
GTCCTAA853.892874E-612.3073251
CTGTCGC853.9491624E-612.2915349
TAGACTG1303.272362E-810.9587155
GTGTAGC1408.194547E-910.8688081
CTATGCC700.001493915810.8543464
CTATACT1451.4459147E-810.4800594
ACTGCTC2053.6379788E-1210.1924968
CCAGGAC1409.6988515E-810.1764343
GGCTAGG750.002622883810.144221
GCTAGTA856.601916E-410.056712
GTCGCCC1054.110263E-59.9502911
GTCTTAG1356.3375046E-79.8624351
GTCCTGG1553.986861E-89.8169871
TAATACT1554.069807E-89.80392554
GAGTATG800.0044735949.5102071
GTGCTAG1606.510345E-89.5102071
CTTCTAA1304.1744643E-69.5102061
CGGGTTG800.0045194599.49845614
Produced by FastQC (version 0.11.5)