FastQCFastQC Report
Wed 25 May 2016
SRR1294528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052534
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33440.31770945166616943No Hit
GGTATCAACGCAGAGTACTTTTTTT26460.25139330415929556No Hit
CCCATGTACTCTGCGTTGATACCAC24150.22944626966919834No Hit
GTACATGGTAAGCAGTGGTATCAAC23740.22555090856922438No Hit
ACCATGTACTCTGCGTTGATACCAC22880.21738015114001066No Hit
TATCAACGCAGAGTACTTTTTTTTT20360.19343793169626824No Hit
GTGGTATCAACGCAGAGTACATGGG17920.17025578271105732No Hit
GTGGTAAGCAGTGGTATCAACGCAG17140.16284509574037515No Hit
ATTCCATTCCATTCCATTCCATTCC13600.129211977950356No Hit
CATGTACTCTGCGTTGATACCACTG13360.1269317665747615No Hit
GGTAAGCAGTGGTATCAACGCAGAG12560.11933106198944643No Hit
GAATGGAATGGAATGGAATGGAATG10990.10441467924076561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG604.070441E-412.6738931
TAGGACC951.0357253E-612.00285154
GTGTTAG1501.7462298E-1011.4065041
GAACTGT803.7618805E-410.6895316
ACTGATC909.512037E-510.5550558
GTTAGCC1106.0209604E-610.3660983
AGTGCTC750.00264740110.1328528
CAAGACT1803.9108272E-1010.0301614
AGGACCT1151.0228163E-59.91492755
GTTGTAG1252.5474164E-69.8856371
GTATTAG1606.561095E-89.505421
GTGTAGC1201.6867523E-59.505421
CTCCTAT1002.7372118E-49.505421
CCCATAC900.00111046779.5022583
GCAAGAC1606.594746E-89.5022573
TGTTAGC1304.2191623E-69.5022572
CTAGGAC800.0045045669.5022573
CTTACCC1106.8113055E-59.5022573
ATACTGG1502.6353155E-79.5018056
ACTTACC800.00451517439.4995498
Produced by FastQC (version 0.11.5)