SRR1294527_2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1294527_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1780262
Sequences flagged as poor quality	0
Sequence length	25
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCAC	4301	0.24159365306904265	No Hit
ACCATGTACTCTGCGTTGATACCAC	4131	0.23204449682125441	No Hit
GTACATGGTAAGCAGTGGTATCAAC	4108	0.230752552152436	No Hit
ATTCCATTCCATTCCATTCCATTCC	3228	0.18132162569329682	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3015	0.16935709462989154	No Hit
GTGGTAAGCAGTGGTATCAACGCAG	2948	0.1655936036381162	No Hit
GTGGTATCAACGCAGAGTACATGGG	2723	0.15295501448663174	No Hit
GAATGGAATGGAATGGAATGGAATG	2550	0.1432373437168237	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	2236	0.12559949041208543	No Hit
CATGTACTCTGCGTTGATACCACTG	2196	0.12335263011848817	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1808	0.101558085270595	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGAA	55	1.9636519E-4	13.815485	12
CTCGTGG	55	1.964588E-4	13.814709	11
CTCTACG	60	4.0973473E-4	12.664906	3
TAGACTG	210	0.0	12.664552	5
CTAGACT	120	7.421477E-10	12.664551	4
GTCTTGC	205	0.0	11.135107	1
GGAACGA	70	0.0014933172	10.855634	15
GCCATAC	90	9.529359E-5	10.554089	3
TAAGACT	160	6.188202E-9	10.092064	4
TAGTACT	190	1.0004442E-10	9.998329	4
CTAATAC	305	0.0	9.654395	3
CTAGCAC	150	2.6529597E-7	9.498679	3
TAATACT	300	0.0	9.498413	4
CAGGACT	240	1.8189894E-12	9.498413	4
GTATTAG	335	0.0	9.369279	1
GTGTTAA	295	0.0	9.35003	1
AGTACTG	255	0.0	9.31217	5
GTCTTGG	250	1.8189894E-12	9.130788	1
GTATTAT	460	0.0	9.097706	1
GTCTAGC	115	1.0963177E-4	9.097706	1