FastQCFastQC Report
Wed 25 May 2016
SRR1294527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780262
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61050.3429270523102779No Hit
CCCATGTACTCTGCGTTGATACCAC44450.24968235012599269No Hit
GTACATGGTAAGCAGTGGTATCAAC43380.2436719988406201No Hit
GGTATCAACGCAGAGTACTTTTTTT42570.2391221067460857No Hit
ACCATGTACTCTGCGTTGATACCAC42230.23721227549652804No Hit
TATCAACGCAGAGTACTTTTTTTTT34590.19429724388882086No Hit
GTGGTAAGCAGTGGTATCAACGCAG33560.18851157863280799No Hit
ATTCCATTCCATTCCATTCCATTCC32520.18266974186945517No Hit
GTGGTATCAACGCAGAGTACATGGG28040.15750490658116614No Hit
GGTAAGCAGTGGTATCAACGCAGAG25970.14587740456180046No Hit
GAATGGAATGGAATGGAATGGAATG25490.1431811722094838No Hit
CATGTACTCTGCGTTGATACCACTG23090.12970001044790036No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19150.1075684365559676No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18150.10195128582197452No Hit
CCATGTACTCTGCGTTGATACCACT18030.10127722773389536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC700.001485636210.8623471
TAAGGTG1553.6507117E-910.4211265
GTATAGG2151.8189894E-1210.1676621
CGCTCAG750.00265033110.1322110
GTCTTAC1909.822543E-1110.0047931
GACTGTT1451.6168087E-79.82760057
CTTAGAC1201.6962944E-59.5018833
TAGACTG2106.730261E-119.5016165
GTATTAC1451.694807E-69.1768091
GTATTGC1356.8045374E-69.1525331
GTATTAA3750.09.1243711
GGACTGT2106.657501E-109.0491576
ACCGTGC1054.495987E-49.046878
GTCTTGC2651.8189894E-128.9665581
GTCTTAG1701.6719059E-78.9454621
AGTACAG3000.08.8681755
CTATGCT2054.12183E-98.8066234
AAGGTGT2054.125468E-98.8063766
TAACCTG2054.125468E-98.8063765
GTTTAGG1304.3434422E-58.7734341
Produced by FastQC (version 0.11.5)