FastQCFastQC Report
Wed 25 May 2016
SRR1294526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061589
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC446032.1635253195472037No Hit
ACCATGTACTCTGCGTTGATACCAC241091.1694377492312968No Hit
GTACATGGTAAGCAGTGGTATCAAC231521.1230172454354383No Hit
GTGGTAAGCAGTGGTATCAACGCAG218401.0593770145261738No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA214361.0397804800083819No Hit
CATGTACTCTGCGTTGATACCACTG208771.012665473088962No Hit
GGTAAGCAGTGGTATCAACGCAGAG163610.7936111416970114No Hit
GTATCAACGCAGAGTACATGGGAAG155980.7566008549715777No Hit
ACTCTGCGTTGATACCACTGCTTAC131660.6386335976763554No Hit
GGTATCAACGCAGAGTACATGGGAA121820.590903424494407No Hit
GTACTCTGCGTTGATACCACTGCTT118560.5750903793142086No Hit
CCATGTACTCTGCGTTGATACCACT118440.5745083040315019No Hit
GTACATGGGAAGCAGTGGTAAGCAG113720.5516133429117055No Hit
GTGGTATCAACGCAGAGTACATGGG108590.5267296245759946No Hit
GCAGTGGTATCAACGCAGAGTACAT101160.49068946332173874No Hit
TATCAACGCAGAGTACATGGGAAGC99610.4831709909201107No Hit
GTACATGGGAAGCAGTGGTATCAAC97020.47060786606835797No Hit
GCTTACCACTGCTTCCCATGTACTC93970.45581345263289624No Hit
GAGTACATGGTAAGCAGTGGTATCA87840.42607910694129625No Hit
AAGCAGTGGTATCAACGCAGAGTAC83280.4039602461984421No Hit
GCGTTGATACCACTGCTTACCACTG80520.39057251469618826No Hit
GCTTACCATGTACTCTGCGTTGATA77420.3755355698929321No Hit
GCGTTGATACCACTGCTTACCATGT76170.3694722856980707No Hit
GTATCAACGCAGAGTACTTTTTTTT71820.3483720566999533No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA70170.34036852156273634No Hit
CTGCTTACCATGTACTCTGCGTTGA66360.32188763133679893No Hit
GAGTAAGCAGTGGTATCAACGCAGA63610.30854840610810397No Hit
CAGTGGTATCAACGCAGAGTACATG62010.3007874023386815No Hit
GTACATAAGCAGTGGTATCAACGCA59060.28647805163880874No Hit
GTATCAACGCAGAGTACATGGTAAG58610.2842952693286586No Hit
GTGATAAGCAGTGGTATCAACGCAG58340.2829855999425686No Hit
ACGCAGAGTACATGGGAAGCAGTGG54720.2654263289142501No Hit
ATGGTAAGCAGTGGTATCAACGCAG54210.2629525089627467No Hit
TATCAACGCAGAGTACATGGTAAGC51820.2513595095821718No Hit
GTACATGATAAGCAGTGGTATCAAC49020.2377777529856824No Hit
TATCAACGCAGAGTACTTTTTTTTT47760.23166596251726215No Hit
GAGTACATGGGAAGCAGTGGTAAGC47160.22875558610372873No Hit
ACGCAGAGTACATGGTAAGCAGTGG46860.22730039789696202No Hit
ATCATGTACTCTGCGTTGATACCAC44280.2147857793187682No Hit
GGTATCAACGCAGAGTACTTTTTTT44000.21342760365911922No Hit
GAGTACATGGGAAGCAGTGGTATCA41210.1998943533361887No Hit
AAAAAGTACTCTGCGTTGATACCAC40970.19873020277077535No Hit
GCTTCCCATGTACTCTGCGTTGATA39160.18995056725661613No Hit
TACCACTGCTTACCATGTACTCTGC38400.18626409046614045No Hit
GTAAGCAGTGGTATCAACGCAGAGT38120.18490591480649152No Hit
GGTATCAACGCAGAGTACATGGTAA37950.18408130815599036No Hit
GTACATGGGAAGTAAGCAGTGGTAT37390.1813649568366925No Hit
GCTTATGTACTCTGCGTTGATACCA35930.17428304089709443No Hit
CTTCCCATGTACTCTGCGTTGATAC35580.17258532132253324No Hit
GCTTACTCTGCGTTGATACCACTGC35500.1721972711340621No Hit
GTGGTATCAACGCAGAGTACATGGT34990.16972345118255872No Hit
AAAGTACTCTGCGTTGATACCACTG33570.1628355603371962No Hit
CTGCTTACCACTGCTTCCCATGTAC31420.1524067115220347No Hit
GATAAGCAGTGGTATCAACGCAGAG30850.14964185392917792No Hit
ACTCTGCGTTGATACCACTGCTTAT29850.14479122657328886No Hit
GCGTTGATACCACTGCTTCCCATGT29490.1430450007251688No Hit
GTACATAGTAAGCAGTGGTATCAAC29320.14222039407466763No Hit
TGGTAAGCAGTGGTATCAACGCAGA27310.13247063308933063No Hit
GCAGAGTACATGGTAAGCAGTGGTA26650.12926921903444383No Hit
ACTATGTACTCTGCGTTGATACCAC26340.12776552455411821No Hit
GTGTAAGCAGTGGTATCAACGCAGA26220.12718344927141154No Hit
GCGTTGATACCACTGCTTACTCTGC26180.12698942417717596No Hit
CTGCTTCCCATGTACTCTGCGTTGA25930.1257767673382037No Hit
GTACATGGTAAGCAGTGGTAAGCAG25460.12349697248093582No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23060.11185546682680204No Hit
GTTGATACCACTGCTTACCATGTAC22580.1095271656959753No Hit
CTGCTTACTCTGCGTTGATACCACT22250.1079264586685319No Hit
CTTATGTACTCTGCGTTGATACCAC22030.1068593206502363No Hit
AAACAAAAAAAAAAAAAAAAAAAAA21390.1037549191424673No Hit
GTTGATACCACTGCTTACCACTGCT21030.10200869329434721No Hit
GTACATGGGAAGCAGTGATAAGCAG20970.10171765565299387No Hit
ATCAACGCAGAGTACATGGGAAGCA20900.10137811173808164No Hit
GCTTACTTCCCATGTACTCTGCGTT20790.10084454272893384No Hit
GCTTACCACTGCTTACCATGTACTC20770.10074753018181606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACT456.7738094E-414.77485315
GAACAAA10000.014.4631071
GCTAGGT500.001502350313.2970453
GCACCGT500.001502899713.2963996
CCCGTGT604.03819E-412.6869361
GTACTAA1001.4135549E-712.3697631
AACGATA550.003075816212.08734412
CGCAAAA1501.4551915E-1112.0356242
TAAGACA1157.1016984E-811.5623674
GTCTTAC1001.8936171E-611.4182421
GCCAGTA752.0707765E-411.402172
TACAGGG1950.011.207262
CCCCTAG600.005807226511.1010691
CTGCCGA600.00588542211.081140514
CTAAGAC600.005886485311.0808733
AGAGGTG1053.4786626E-610.8542035
GGTTGTC700.001496040310.8534158
GTACAAA2200.010.812731
ACAACGT1502.1464075E-910.7629710
GTCTTGC3450.010.7563151
Produced by FastQC (version 0.11.5)