FastQCFastQC Report
Wed 25 May 2016
SRR1294526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061589
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC465872.259761766288043No Hit
GTGGTAAGCAGTGGTATCAACGCAG247881.2023735089777836No Hit
ACCATGTACTCTGCGTTGATACCAC247601.2010153333181346No Hit
GTATCAACGCAGAGTACTTTTTTTT246361.1950005553968321No Hit
GTACATGGTAAGCAGTGGTATCAAC244431.1856388445999664No Hit
CATGTACTCTGCGTTGATACCACTG218381.059280001979056No Hit
GGTAAGCAGTGGTATCAACGCAGAG185440.8995003368760699No Hit
GTATCAACGCAGAGTACATGGGAAG162530.7883724641526513No Hit
TATCAACGCAGAGTACTTTTTTTTT158900.7707646868507738No Hit
GGTATCAACGCAGAGTACTTTTTTT148300.7193480368783496No Hit
ACTCTGCGTTGATACCACTGCTTAC137530.6671067802554244No Hit
GGTATCAACGCAGAGTACATGGGAA127840.6201042011768592No Hit
CCATGTACTCTGCGTTGATACCACT127010.6160781804714713No Hit
GTACTTTTTTTTTTTTTTTTTTTTT124720.6049702438264853No Hit
GTACATGGGAAGCAGTGGTAAGCAG121010.5869744163361368No Hit
GTACTCTGCGTTGATACCACTGCTT119520.5797469815758621No Hit
GTGGTATCAACGCAGAGTACATGGG113710.5515648366381466No Hit
GCAGTGGTATCAACGCAGAGTACAT108990.5286698755183502No Hit
TATCAACGCAGAGTACATGGGAAGC108420.5259050179254934No Hit
AAGCAGTGGTATCAACGCAGAGTAC99670.483462028561464No Hit
GTACATGGGAAGCAGTGGTATCAAC98820.47933899530895824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA97490.47288766092562584No Hit
GCTTACCACTGCTTCCCATGTACTC95870.4650296446090855No Hit
GAGTACATGGTAAGCAGTGGTATCA91470.44368688424317354No Hit
GCGTTGATACCACTGCTTACCACTG83250.4038147273777654No Hit
ACGCAGAGTACTTTTTTTTTTTTTT79860.3873711006413014No Hit
GCGTTGATACCACTGCTTACCATGT78910.38276300465320684No Hit
GAGTACTTTTTTTTTTTTTTTTTTT78780.38213242309694123No Hit
GCTTACCATGTACTCTGCGTTGATA77480.37582660753428543No Hit
CAGTGGTATCAACGCAGAGTACATG69740.338282751799704No Hit
CTGCTTACCATGTACTCTGCGTTGA67490.3273688402489536No Hit
GAGTAAGCAGTGGTATCAACGCAGA66310.3216450999690045No Hit
GTGATAAGCAGTGGTATCAACGCAG65520.31781310435785215No Hit
GTATCAACGCAGAGTACATGGTAAG61570.2986531263020903No Hit
ACGCAGAGTACATGGGAAGCAGTGG61520.29841059493429584No Hit
GTACATAAGCAGTGGTATCAACGCA60620.29404503031399565No Hit
ATGGTAAGCAGTGGTATCAACGCAG60480.2933659424841712No Hit
TATCAACGCAGAGTACATGGTAAGC53670.26033317019056657No Hit
ACGCAGAGTACATGGTAAGCAGTGG53150.2578108439655043No Hit
GAGTACATGGGAAGCAGTGGTAAGC50530.24510220029307492No Hit
GTACATGATAAGCAGTGGTATCAAC50200.2435014932656315No Hit
ATCATGTACTCTGCGTTGATACCAC46500.22555417204884193No Hit
GTAAGCAGTGGTATCAACGCAGAGT43490.21095378370761583No Hit
GAGTACATGGGAAGCAGTGGTATCA42900.20809191356764128No Hit
GGTATCAACGCAGAGTACATGGTAA42120.2043084242300478No Hit
GTGGTATCAACGCAGAGTACTTTTT41940.20343531130598774No Hit
AAAAAGTACTCTGCGTTGATACCAC41810.2028047297497222No Hit
GCTTCCCATGTACTCTGCGTTGATA40140.19470418206538742No Hit
TACCACTGCTTACCATGTACTCTGC38270.18563350890987484No Hit
CTTCCCATGTACTCTGCGTTGATAC38250.18553649636275707No Hit
GTACATGGGAAGTAAGCAGTGGTAT37420.18151047565736914No Hit
GCTTACTCTGCGTTGATACCACTGC36300.1760777730187734No Hit
GCTTATGTACTCTGCGTTGATACCA36040.17481660990624223No Hit
GTGGTATCAACGCAGAGTACATGGT35360.17151818330423765No Hit
GCAGAGTACTTTTTTTTTTTTTTTT34390.16681307476902524No Hit
AAAGTACTCTGCGTTGATACCACTG33830.16409672344972737No Hit
GATAAGCAGTGGTATCAACGCAGAG33660.1632721167992262No Hit
CTGCTTACCACTGCTTCCCATGTAC31780.15415293737015476No Hit
ACTCTGCGTTGATACCACTGCTTAT31140.15104853586238576No Hit
GCAGAGTACATGGTAAGCAGTGGTA30110.14605238968582002No Hit
GTACATAGTAAGCAGTGGTATCAAC29870.14488823912040663No Hit
TGGTAAGCAGTGGTATCAACGCAGA29710.1441121387434644No Hit
GCGTTGATACCACTGCTTCCCATGT29150.1413957874241665No Hit
GTGTAAGCAGTGGTATCAACGCAGA27600.13387731502253844No Hit
ACTATGTACTCTGCGTTGATACCAC27170.13179154525950615No Hit
CTGCTTCCCATGTACTCTGCGTTGA27090.131403495071035No Hit
GTACATGGTAAGCAGTGGTAAGCAG26510.12859013120461935No Hit
GCGTTGATACCACTGCTTACTCTGC26250.12732896809208819No Hit
GCAGTGGTATCAACGCAGAGTACTT26120.1266983865358226No Hit
GCTAAGCAGTGGTATCAACGCAGAG23500.11398974286339324No Hit
CTGCTTACTCTGCGTTGATACCACT23300.11301961739221542No Hit
ATCAACGCAGAGTACATGGGAAGCA23260.11282559229797987No Hit
ATCAACGCAGAGTACTTTTTTTTTT23030.11170994800612537No Hit
CTTATGTACTCTGCGTTGATACCAC21990.10666529555600074No Hit
GTTGATACCACTGCTTACCATGTAC21880.10613172654685293No Hit
CTGCTAAGCAGTGGTATCAACGCAG21850.10598620772617627No Hit
GCTTACCACTGCTTACCATGTACTC21610.1048220571607629No Hit
GCAGTGGTAAGCAGTGGTATCAACG21220.10293031249196614No Hit
GATACCACTGCTTACCATGTACTCT21100.10234823720925947No Hit
AAGCAGTGGTAAGCAGTGGTATCAA20810.10094155527605163No Hit
GTACATGGGAAGCAGTGATAAGCAG20760.10069902390825718No Hit
GCTTACTTCCCATGTACTCTGCGTT20680.10031097371978606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC350.002166138716.2906443
TCTCCCG551.9640828E-413.81532513
TAACCTA500.00149669413.3037025
GAACCAT957.361996E-813.0039346
AACGTTG604.0985033E-412.66466112
TTAACGC658.0050196E-411.69584754
GAACAAA4450.011.5352181
GTCGATA752.0686188E-411.403453
TAAAGGT752.0686188E-411.403454
TCGATAC752.0686188E-411.403454
GTATTAG1104.9310256E-711.2342161
AGACTGT700.001487997810.860436
ATTAACG700.001487997810.860433
CTGCTAA4150.010.7656891
TGGACTG803.7621096E-410.6904755
ACGTTGA1701.4006218E-1010.61545213
CTTGCTC2900.010.4859323
ATGATAC2001.8189894E-1210.44758616
TTTTTAC3050.010.2850451
GCTTATA2700.010.2100131
Produced by FastQC (version 0.11.5)