FastQCFastQC Report
Wed 25 May 2016
SRR1294525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851321
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC353721.2405478022292125No Hit
ACCATGTACTCTGCGTTGATACCAC269770.9461228672604732No Hit
GTACATGGTAAGCAGTGGTATCAAC255990.8977943907402919No Hit
GTGGTAAGCAGTGGTATCAACGCAG202750.7110739197726247No Hit
CATGTACTCTGCGTTGATACCACTG181440.6363366313368435No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA169850.5956888052941076No Hit
GGTAAGCAGTGGTATCAACGCAGAG158140.5546201216909635No Hit
CCATGTACTCTGCGTTGATACCACT123850.43436007380438746No Hit
GTACATGGGAAGCAGTGGTAAGCAG116520.4086526911561343No Hit
GTATCAACGCAGAGTACATGGGAAG113310.39739475141522124No Hit
ACTCTGCGTTGATACCACTGCTTAC108760.38143723558308584No Hit
GTACTCTGCGTTGATACCACTGCTT102760.3603943575626876No Hit
GCAGTGGTATCAACGCAGAGTACAT97960.343560055146369No Hit
GCTTACCACTGCTTCCCATGTACTC96490.33840455003137143No Hit
GAGTACATGGTAAGCAGTGGTATCA91980.3225873200527054No Hit
GTGGTATCAACGCAGAGTACATGGG89360.3133985966504648No Hit
GGTATCAACGCAGAGTACATGGGAA89340.31332845372373014No Hit
GTACATGATAAGCAGTGGTATCAAC88490.31034737933750706No Hit
GCTTACCATGTACTCTGCGTTGATA86730.30417480178485695No Hit
GCGTTGATACCACTGCTTACCATGT84360.2958628649667996No Hit
ATCATGTACTCTGCGTTGATACCAC81840.2870248561982323No Hit
GCGTTGATACCACTGCTTACCACTG76340.2677355513462006No Hit
TATCAACGCAGAGTACATGGGAAGC74360.2607914015994692No Hit
GTACATGGGAAGCAGTGGTATCAAC74160.26008997233212255No Hit
AAGCAGTGGTATCAACGCAGAGTAC73270.2569686120924301No Hit
CTGCTTACCATGTACTCTGCGTTGA72350.25374203746263574No Hit
GTATCAACGCAGAGTACATGGTAAG66420.2329446596858088No Hit
GTACATAAGCAGTGGTATCAACGCA65770.23066501456693228No Hit
CAGTGGTATCAACGCAGAGTACATG57670.20225712923939465No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA55810.19573383705307118No Hit
ATGGTAAGCAGTGGTATCAACGCAG54890.1925072624232768No Hit
TATCAACGCAGAGTACATGGTAAGC54790.19215654778960348No Hit
ACGCAGAGTACATGGTAAGCAGTGG51650.18114410829226174No Hit
ACGCAGAGTACATGGGAAGCAGTGG49370.1731478146445104No Hit
GAGTACATGGGAAGCAGTGGTAAGC47210.16557237855716703No Hit
GTGATAAGCAGTGGTATCAACGCAG46840.16427473441257578No Hit
GTATCAACGCAGAGTACTTTTTTTT44590.15638365515492644No Hit
GGTATCAACGCAGAGTACATGGTAA42800.15010586321217428No Hit
TACCACTGCTTACCATGTACTCTGC40720.14281099883176956No Hit
GCTTATGTACTCTGCGTTGATACCA39080.13705927883952737No Hit
GTGGTATCAACGCAGAGTACATGGT37510.1315530590908565No Hit
GCTTCCCATGTACTCTGCGTTGATA35040.12289040763912586No Hit
GATAAGCAGTGGTATCAACGCAGAG34790.12201362105494261No Hit
ACTCTGCGTTGATACCACTGCTTAT33000.11573582911219046No Hit
GAGTACATGATAAGCAGTGGTATCA32290.11324575521310999No Hit
AAAAAGTACTCTGCGTTGATACCAC31630.11093103863086617No Hit
CTGCTTACCACTGCTTCCCATGTAC31120.10914239399913232No Hit
GAGTACATGGGAAGCAGTGGTATCA30510.10700303473372516No Hit
GCTTATCATGTACTCTGCGTTGATA29300.10275938766627819No Hit
GTACATGGTAAGCAGTGGTAAGCAG29170.10230345864250288No Hit
TATCAACGCAGAGTACTTTTTTTTT28600.10030438523056506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC456.76743E-414.77701416
TGCGCAA500.001503247713.2962810
GAACAAA8950.012.6470491
TAAGGCT853.955054E-612.2917584
GCGCATA550.003075559212.087749
AGACCGT1302.6302587E-911.6892426
TCGTGGG1900.011.49678312
GTATTAG1751.8189894E-1211.41422751
TCGACCG752.0800317E-411.3970119
TATAACG600.00587535311.0839272
AGCCTCG2900.010.8109242
ACCGTGC1158.8542765E-710.7363158
TAGGGGT1252.2481618E-710.6379574
TCCTATA750.002647554310.1338762
GCATAAC856.5213244E-410.0713781
TCTATCC1054.1161496E-59.950473
GACTGTG3150.09.9497727
GCGTTAA1151.0119889E-59.9254151
GCCTCGG3450.09.91113953
CCGTGCA1252.580442E-69.877419
Produced by FastQC (version 0.11.5)