FastQCFastQC Report
Wed 25 May 2016
SRR1294525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851321
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC374701.3141277323738716No Hit
ACCATGTACTCTGCGTTGATACCAC278550.9769156120969895No Hit
GTACATGGTAAGCAGTGGTATCAAC267100.9367587865413961No Hit
GTGGTAAGCAGTGGTATCAACGCAG225390.7904757128362608No Hit
CATGTACTCTGCGTTGATACCACTG192820.6762479566488655No Hit
GGTAAGCAGTGGTATCAACGCAGAG174480.6119268928331816No Hit
GTATCAACGCAGAGTACTTTTTTTT144140.505520072976701No Hit
CCATGTACTCTGCGTTGATACCACT133350.4676779640033514No Hit
GTATCAACGCAGAGTACATGGGAAG122120.4282927106418394No Hit
GTACATGGGAAGCAGTGGTAAGCAG120240.4216992755287812No Hit
ACTCTGCGTTGATACCACTGCTTAC115730.4058820455501152No Hit
GCAGTGGTATCAACGCAGAGTACAT105880.37133665413329475No Hit
GTACTCTGCGTTGATACCACTGCTT104270.3656901485311545No Hit
GCTTACCACTGCTTCCCATGTACTC99810.35004827586932513No Hit
GAGTACATGGTAAGCAGTGGTATCA99620.34938191806534585No Hit
GGTATCAACGCAGAGTACATGGGAA93100.3265153239498464No Hit
GTGGTATCAACGCAGAGTACATGGG91440.32069346103086954No Hit
GCTTACCATGTACTCTGCGTTGATA90350.3168706715238305No Hit
TATCAACGCAGAGTACTTTTTTTTT90290.31666024274362653No Hit
GTACATGATAAGCAGTGGTATCAAC88290.30964595007016044No Hit
GCGTTGATACCACTGCTTACCATGT87250.3059985178799581No Hit
GGTATCAACGCAGAGTACTTTTTTT86640.30385915861455093No Hit
ATCATGTACTCTGCGTTGATACCAC85250.298984225206492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA83800.2938988630182291No Hit
AAGCAGTGGTATCAACGCAGAGTAC83290.29211021838649526No Hit
TATCAACGCAGAGTACATGGGAAGC81210.28481535400609054No Hit
GCGTTGATACCACTGCTTACCACTG79360.27832713328313435No Hit
GTACATGGGAAGCAGTGGTATCAAC77500.27180384109681094No Hit
CTGCTTACCATGTACTCTGCGTTGA75360.26429854793620217No Hit
GTACTTTTTTTTTTTTTTTTTTTTT72560.2544785381933497No Hit
GTATCAACGCAGAGTACATGGTAAG71460.2506206772229433No Hit
GTACATAAGCAGTGGTATCAACGCA66650.2337513033432574No Hit
CAGTGGTATCAACGCAGAGTACATG62250.21831985946163202No Hit
ATGGTAAGCAGTGGTATCAACGCAG59550.20885056435245275No Hit
TATCAACGCAGAGTACATGGTAAGC56940.19969691241357954No Hit
ACGCAGAGTACATGGGAAGCAGTGG56240.1972419099778664No Hit
ACGCAGAGTACATGGTAAGCAGTGG56230.19720683851449905No Hit
GTGATAAGCAGTGGTATCAACGCAG50570.17735639024859004No Hit
GAGTACATGGGAAGCAGTGGTAAGC50430.17686538976144742No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48590.1704122405018586No Hit
GAGTACTTTTTTTTTTTTTTTTTTT46090.161644374660026No Hit
GGTATCAACGCAGAGTACATGGTAA45650.16010123027186346No Hit
TACCACTGCTTACCATGTACTCTGC42580.149334291018093No Hit
GCTTATGTACTCTGCGTTGATACCA41840.14673900272891058No Hit
GTGGTATCAACGCAGAGTACATGGT38690.13569149176820147No Hit
GATAAGCAGTGGTATCAACGCAGAG37580.1317985593344278No Hit
GCTTCCCATGTACTCTGCGTTGATA36410.12769519812045016No Hit
ACTCTGCGTTGATACCACTGCTTAT35190.12341647958963582No Hit
GAGTACATGATAAGCAGTGGTATCA34130.1196989044726988No Hit
GAGTACATGGGAAGCAGTGGTATCA33080.11601640081912909No Hit
AAAAAGTACTCTGCGTTGATACCAC32710.11471875667453788No Hit
CTGCTTACCACTGCTTCCCATGTAC30980.1086513935119897No Hit
GCAGAGTACATGGTAAGCAGTGGTA30880.1083006788783164No Hit
TGGTAAGCAGTGGTATCAACGCAGA30410.10665232010005188No Hit
GTAAGCAGTGGTATCAACGCAGAGT30190.10588074790597062No Hit
GTACATGGTAAGCAGTGGTAAGCAG30160.1057755335158686No Hit
GTGGTATCAACGCAGAGTACTTTTT29080.10198781547219693No Hit
GCTTATCATGTACTCTGCGTTGATA28890.10132145766821765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAC250.00603579718.998638
GTATCGT400.00527384214.2527246
GCGTTAT551.950898E-413.8266431
TCGCCGG604.1000138E-412.66442117
CACTCGT550.00306956912.0908877
CGCGTAA550.003071989212.08961510
TGGACCG600.00586934411.0854515
TCGCGTA600.005880849411.0825359
ACTATAC1158.8027446E-710.7411833
CTATTAG1355.6423232E-810.5620191
ACGGACC909.5275034E-510.5547958
TAGAGCT2500.010.2619614
TAATGGT1606.159098E-910.0956794
CCAGTAC2450.010.083563
CTAGACC951.6417462E-410.0019113
CAGTACT2105.456968E-129.9542834
CATAAGC16100.09.7378854
CTGCTAA4000.09.5058171
ATTAGAC800.0045102849.5018163
CCGACCC1106.825783E-59.5018164
Produced by FastQC (version 0.11.5)