FastQCFastQC Report
Wed 25 May 2016
SRR1294524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583369
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC364441.410716006888679No Hit
GTACATGGTAAGCAGTGGTATCAAC249350.9652124802922076No Hit
ACCATGTACTCTGCGTTGATACCAC248000.9599867459894424No Hit
GTGGTAAGCAGTGGTATCAACGCAG192350.7445703652865696No Hit
CATGTACTCTGCGTTGATACCACTG178830.6922356039729516No Hit
GGTAAGCAGTGGTATCAACGCAGAG152920.591940214502845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA140620.5443279686332072No Hit
GTATCAACGCAGAGTACATGGGAAG131080.5073994462270004No Hit
CCATGTACTCTGCGTTGATACCACT118590.4590517266406773No Hit
ACTCTGCGTTGATACCACTGCTTAC113110.43783911628574934No Hit
GTACTCTGCGTTGATACCACTGCTT102390.396342915007496No Hit
GGTATCAACGCAGAGTACATGGGAA100130.3875946486932374No Hit
GTACATGGGAAGCAGTGGTAAGCAG97530.3775302715175416No Hit
GTGGTATCAACGCAGAGTACATGGG95010.36777556748571344No Hit
GCAGTGGTATCAACGCAGAGTACAT90560.35054999885808025No Hit
GAGTACATGGTAAGCAGTGGTATCA88120.34110496797011963No Hit
GCTTACCACTGCTTCCCATGTACTC81940.3171827176063505No Hit
GCGTTGATACCACTGCTTACCATGT81890.3169891718914332No Hit
TATCAACGCAGAGTACATGGGAAGC81880.3169504627484498No Hit
GCTTACCATGTACTCTGCGTTGATA81360.3149375873133106No Hit
GCGTTGATACCACTGCTTACCACTG81150.3141246953106583No Hit
GTACATGGGAAGCAGTGGTATCAAC74570.2886540792275513No Hit
CTGCTTACCATGTACTCTGCGTTGA70610.27332525860610696No Hit
AAGCAGTGGTATCAACGCAGAGTAC68600.2655447208664345No Hit
GTATCAACGCAGAGTACATGGTAAG64520.24975139052918882No Hit
GTACATAAGCAGTGGTATCAACGCA57890.22408722873116463No Hit
CAGTGGTATCAACGCAGAGTACATG55590.2151841258449722No Hit
TATCAACGCAGAGTACATGGTAAGC53500.20709391496143212No Hit
ATGGTAAGCAGTGGTATCAACGCAG52830.20450040238154132No Hit
ACGCAGAGTACATGGTAAGCAGTGG52630.20372621952187242No Hit
ACGCAGAGTACATGGGAAGCAGTGG49810.19281024120054086No Hit
GTACATGATAAGCAGTGGTATCAAC44030.1704363565561095No Hit
GTGATAAGCAGTGGTATCAACGCAG42630.16501707653842715No Hit
GGTATCAACGCAGAGTACATGGTAA42490.16447514853665893No Hit
ATCATGTACTCTGCGTTGATACCAC42470.16439773025069201No Hit
GTATCAACGCAGAGTACTTTTTTTT41810.16184292681378465No Hit
GAGTACATGGGAAGCAGTGGTAAGC41180.1594042508058276No Hit
TACCACTGCTTACCATGTACTCTGC39730.15379142507322802No Hit
GTGGTATCAACGCAGAGTACATGGT37860.14655281533532377No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA37630.1456625050467045No Hit
GCTTATGTACTCTGCGTTGATACCA35400.13703036616139622No Hit
AAAAAGTACTCTGCGTTGATACCAC35110.1359078010148763No Hit
GCTTCCCATGTACTCTGCGTTGATA31460.12177896382591878No Hit
GAGTACATGGGAAGCAGTGGTATCA31260.12100478096624988No Hit
AAAGTACTCTGCGTTGATACCACTG29450.11399842608624629No Hit
CTTCCCATGTACTCTGCGTTGATAC28190.10912107407033218No Hit
GCAGAGTACATGGTAAGCAGTGGTA27890.10795979978082883No Hit
TATCAACGCAGAGTACTTTTTTTTT27070.10478565005618633No Hit
TGGTAAGCAGTGGTATCAACGCAGA27060.10474694091320287No Hit
GGTATCAACGCAGAGTACTTTTTTT26850.10393404891055051No Hit
GTAAGCAGTGGTATCAACGCAGAGT26700.10335341176579885No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT26660.10319857519386506No Hit
ACTCTGCGTTGATACCACTGCTTAT26010.10068248089994113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTA400.00528642814.2475317
GAACAAA6150.012.8353191
TGTACCG701.0934039E-412.2119335
CGAATAA550.003060920412.09537119
GAACCGC855.3351945E-511.1745366
AGCACCG1451.2223609E-911.1357855
ACAACCG600.005885874411.08125
CCGCGCG600.00588672311.0809849
GTAAGAC1408.374627E-910.8558923
TAGAAGT2100.010.4031614
GTATTAG1659.131327E-1010.3750991
GTGCTAG1105.969796E-610.3750991
GTGTAAG2700.010.2149891
AAACGTT750.002652394810.1315786
TATTCCG750.00265282110.1313825
ACATAAG12250.09.9253873
CTAGACA1252.576522E-69.878484
GGTTATA1356.3501466E-79.8627481
GTATTAC1553.997411E-89.8172981
ACCTATA1554.0528903E-89.8085522
Produced by FastQC (version 0.11.5)