FastQCFastQC Report
Wed 25 May 2016
SRR1294524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583369
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC386461.4959535397382255No Hit
ACCATGTACTCTGCGTTGATACCAC263791.0211084827603025No Hit
GTACATGGTAAGCAGTGGTATCAAC245690.9510449339602666No Hit
GTGGTAAGCAGTGGTATCAACGCAG214230.8292659701343479No Hit
CATGTACTCTGCGTTGATACCACTG186920.7235513006465588No Hit
GGTAAGCAGTGGTATCAACGCAGAG167100.6468297792533704No Hit
GTATCAACGCAGAGTACATGGGAAG134210.5195154079808189No Hit
GTATCAACGCAGAGTACTTTTTTTT133760.5177734965465638No Hit
CCATGTACTCTGCGTTGATACCACT126680.49036742331428457No Hit
ACTCTGCGTTGATACCACTGCTTAC118830.45998074607228No Hit
GTACATGGGAAGCAGTGGTAAGCAG105710.4091943504779999No Hit
GTACTCTGCGTTGATACCACTGCTT103950.4023815413129135No Hit
GGTATCAACGCAGAGTACATGGGAA103560.40087188473655916No Hit
GTGGTATCAACGCAGAGTACATGGG95920.37129809949720693No Hit
GAGTACATGGTAAGCAGTGGTATCA93960.36371110747245167No Hit
GCAGTGGTATCAACGCAGAGTACAT93560.36216274175311386No Hit
TATCAACGCAGAGTACTTTTTTTTT87250.3377372725305599No Hit
GCGTTGATACCACTGCTTACCATGT86780.335917942810338No Hit
TATCAACGCAGAGTACATGGGAAGC85240.32995673479088744No Hit
GCTTACCACTGCTTCCCATGTACTC85000.3290277153592847No Hit
GGTATCAACGCAGAGTACTTTTTTT84980.32895029707331785No Hit
GCTTACCATGTACTCTGCGTTGATA84670.327750313640831No Hit
GCGTTGATACCACTGCTTACCACTG82370.3188472107546386No Hit
GTACATGGGAAGCAGTGGTATCAAC78440.30363451756214466No Hit
AAGCAGTGGTATCAACGCAGAGTAC78210.3027442072735254No Hit
CTGCTTACCATGTACTCTGCGTTGA74480.2883056969407003No Hit
GTACTTTTTTTTTTTTTTTTTTTTT69440.26879628887704393No Hit
GTATCAACGCAGAGTACATGGTAAG66060.25571259854863937No Hit
CAGTGGTATCAACGCAGAGTACATG60950.23593222648409887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA60920.23581609905514853No Hit
GTACATAAGCAGTGGTATCAACGCA58860.2278420156005588No Hit
ACGCAGAGTACATGGTAAGCAGTGG56790.21982922300298563No Hit
TATCAACGCAGAGTACATGGTAAGC55530.21495187098707152No Hit
ACGCAGAGTACATGGGAAGCAGTGG55090.21324866869579995No Hit
ATGGTAAGCAGTGGTATCAACGCAG54700.21173901211944557No Hit
GTGATAAGCAGTGGTATCAACGCAG47630.18437164803014977No Hit
GTACATGATAAGCAGTGGTATCAAC45960.17790722115191443No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44810.1734556697088182No Hit
GGTATCAACGCAGAGTACATGGTAA44320.17155892170262937No Hit
ATCATGTACTCTGCGTTGATACCAC44200.17109441198682804No Hit
GAGTACTTTTTTTTTTTTTTTTTTT43560.16861702683588756No Hit
GAGTACATGGGAAGCAGTGGTAAGC43520.1684621902639538No Hit
TACCACTGCTTACCATGTACTCTGC43050.16664286054373184No Hit
GCTTATGTACTCTGCGTTGATACCA36750.14225610046416134No Hit
GTGGTATCAACGCAGAGTACATGGT36430.14101740788869108No Hit
AAAAAGTACTCTGCGTTGATACCAC35510.13745616673421412No Hit
GCTTCCCATGTACTCTGCGTTGATA33630.13017884785332642No Hit
GAGTACATGGGAAGCAGTGGTATCA33090.12808855413222037No Hit
GTGGTATCAACGCAGAGTACTTTTT30740.11899190553111072No Hit
GCAGAGTACATGGTAAGCAGTGGTA29920.11581775580646822No Hit
CTTCCCATGTACTCTGCGTTGATAC29790.11531453694768343No Hit
GTAAGCAGTGGTATCAACGCAGAGT29460.11403713522922974No Hit
TGGTAAGCAGTGGTATCAACGCAGA27830.10772754492292816No Hit
AAAGTACTCTGCGTTGATACCACTG27620.10691465292027583No Hit
CTGCTTACCACTGCTTCCCATGTAC27450.10625659748955724No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT27290.1056372512018221No Hit
GTACATGGTAAGCAGTGGTAAGCAG26800.10374050319563329No Hit
ACTCTGCGTTGATACCACTGCTTAT26530.10269535633508028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT456.7512115E-414.7816046
GTCTACG550.003054573212.0987241
CACCGGA802.8746976E-511.87232717
GTCTAAG1406.730261E-1011.5431711
ACTATAC1251.8277206E-811.402513
CCCGTCA752.0773042E-411.39853712
CGATAGG1001.935805E-611.39765515
TCGAATA752.0787986E-411.39765418
GTCTTAG855.2908537E-511.1836951
GTATTAT1803.6379788E-1211.0904981
ACTAGGT600.00586782111.0857733
TAGGCCC600.00586782111.0857734
GCGATAG1053.4754576E-610.855329514
GTCTATA1252.2220047E-710.6468771
CTATACT1355.6739736E-810.5578794
GTATTAC1451.4302714E-810.4895341
CTAGGTA750.002643755210.1355644
CAACGTT750.002651408810.13203211
CCGTACA750.002653538210.13105217
GTAATAA4050.010.0929391
Produced by FastQC (version 0.11.5)