FastQCFastQC Report
Wed 25 May 2016
SRR1294523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775231
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC545093.070529976098885No Hit
ACCATGTACTCTGCGTTGATACCAC394672.223203628147548No Hit
GTACATGGTAAGCAGTGGTATCAAC386262.1758295117649475No Hit
CATGTACTCTGCGTTGATACCACTG283751.5983835343118726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA280861.5821039628082203No Hit
GTGGTAAGCAGTGGTATCAACGCAG260941.4698932138972336No Hit
GGTAAGCAGTGGTATCAACGCAGAG239931.3515424189865994No Hit
GTATCAACGCAGAGTACATGGGAAG186511.0506238343066339No Hit
CCATGTACTCTGCGTTGATACCACT185591.0454414101601426No Hit
ACTCTGCGTTGATACCACTGCTTAC174190.9812244153014453No Hit
GTACTCTGCGTTGATACCACTGCTT160750.9055159582048758No Hit
GGTATCAACGCAGAGTACATGGGAA146890.8274416118240386No Hit
GCAGTGGTATCAACGCAGAGTACAT143180.8065429231463398No Hit
GAGTACATGGTAAGCAGTGGTATCA141910.7993889245962918No Hit
GTACATGGGAAGCAGTGGTAAGCAG140520.7915589576793105No Hit
GCTTACCATGTACTCTGCGTTGATA129840.7313977730222151No Hit
GCGTTGATACCACTGCTTACCATGT126550.7128649736287841No Hit
TATCAACGCAGAGTACATGGGAAGC122140.6880231361439723No Hit
GTACATGGGAAGCAGTGGTATCAAC117230.6603647637969368No Hit
GCTTACCACTGCTTCCCATGTACTC116930.6586748428796028No Hit
CTGCTTACCATGTACTCTGCGTTGA112350.6328753835416349No Hit
GTGGTATCAACGCAGAGTACATGGG111840.6300025179821668No Hit
GCGTTGATACCACTGCTTACCACTG110090.6201446459643843No Hit
AAGCAGTGGTATCAACGCAGAGTAC106190.5981756740390406No Hit
GTACATGATAAGCAGTGGTATCAAC100170.5642645942978688No Hit
GTATCAACGCAGAGTACATGGTAAG100020.5634196338392018No Hit
ATCATGTACTCTGCGTTGATACCAC94100.5300718610704748No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA91490.5153695490896677No Hit
ATGGTAAGCAGTGGTATCAACGCAG90210.5081592198423754No Hit
CAGTGGTATCAACGCAGAGTACATG84810.477740643330361No Hit
TATCAACGCAGAGTACATGGTAAGC83890.4725582191838696No Hit
ACGCAGAGTACATGGTAAGCAGTGG80470.45329312072626043No Hit
ACGCAGAGTACATGGGAAGCAGTGG75290.42411381955362426No Hit
GTACATAAGCAGTGGTATCAACGCA72750.4098058224535286No Hit
GTGATAAGCAGTGGTATCAACGCAG71700.40389109924285915No Hit
GGTATCAACGCAGAGTACATGGTAA67770.38175313522578186No Hit
TACCACTGCTTACCATGTACTCTGC62870.35415109357599095No Hit
GTGGTATCAACGCAGAGTACATGGT59230.3336467197790034No Hit
GAGTACATGGGAAGCAGTGGTAAGC58580.3299852244581128No Hit
AAAAAGTACTCTGCGTTGATACCAC51020.2873992173412925No Hit
GAGTACATGGGAAGCAGTGGTATCA50480.28435735969009107No Hit
GAGTAAGCAGTGGTATCAACGCAGA49440.27849896717666606No Hit
GCTTCCCATGTACTCTGCGTTGATA48220.2716266221128405No Hit
GATAAGCAGTGGTATCAACGCAGAG46470.26176875009505807No Hit
GCAGAGTACATGGTAAGCAGTGGTA45050.2537697910863431No Hit
GTAAGCAGTGGTATCAACGCAGAGT44590.2511785790130974No Hit
GCTTATGTACTCTGCGTTGATACCA43570.24543284789416137No Hit
GTATCAACGCAGAGTACTTTTTTTT43140.2430106279126491No Hit
AAAGTACTCTGCGTTGATACCACTG42920.24177135257327076No Hit
ACTCTGCGTTGATACCACTGCTTAT42110.23720856609646856No Hit
TGGTAAGCAGTGGTATCAACGCAGA41850.2357439679681123No Hit
CTGCTTACCACTGCTTCCCATGTAC39360.22171762435423897No Hit
CTTCCCATGTACTCTGCGTTGATAC38330.21591556253805844No Hit
GAGTACATGATAAGCAGTGGTATCA38170.21501427138214688No Hit
GTTGATACCACTGCTTACCATGTAC37070.20881789468525502No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT36780.20718430446516536No Hit
GCGTTGATACCACTGCTTCCCATGT36110.2034101477497858No Hit
GATACCACTGCTTACCATGTACTCT35130.19788973941982763No Hit
GCTTATCATGTACTCTGCGTTGATA34680.1953548580438264No Hit
CTGCTTCCCATGTACTCTGCGTTGA33600.1892711427414235No Hit
GTACATGGTAAGCAGTGGTAAGCAG33220.1871305762461336No Hit
ATACCACTGCTTACCATGTACTCTG32200.18138484512719755No Hit
AAACAAAAAAAAAAAAAAAAAAAAA30640.17259725635706No Hit
GAACAAAAAAAAAAAAAAAAAAAAA30390.17118898892594822No Hit
CCACTGCTTACCATGTACTCTGCGT30050.1692737452196362No Hit
TATCAACGCAGAGTACTTTTTTTTT29480.16606289547670133No Hit
GCTTACTCTGCGTTGATACCACTGC29380.16549958850425664No Hit
GCGTTGATACCACTGCTTATCATGT29290.1649926122290564No Hit
GTTGATACCACTGCTTACCACTGCT27970.15755696019278617No Hit
GGTATCAACGCAGAGTACTTTTTTT27810.15665566903687464No Hit
TGATACCACTGCTTACCATGTACTC27710.15609236206442992No Hit
CATGGTAAGCAGTGGTATCAACGCA27310.15383913417465106No Hit
ATCAACGCAGAGTACATGGGAAGCA26480.14916368630335997No Hit
GCTTACCACTGCTTACCATGTACTC26290.148093403055715No Hit
CTTATGTACTCTGCGTTGATACCAC25190.14189702635882318No Hit
GTACATGGGAAGCAGTGATAAGCAG25170.14178436496433422No Hit
TACCACTGCTTACCACTGCTTCCCA25100.1413900500836229No Hit
GCAGTGGTAAGCAGTGGTATCAACG24960.14060142032220033No Hit
TACCACTGCTTCCCATGTACTCTGC24400.1374469012765099No Hit
CCACTGCTTCCCATGTACTCTGCGT24390.13739057057926546No Hit
AAGCAGTGGTAAGCAGTGGTATCAA24020.13530633478122003No Hit
GTACATGGGTAAGCAGTGGTATCAA24000.13519367338673108No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23910.13468669711153083No Hit
GCTTATCACTGCTTCCCATGTACTC23860.13440504362530847No Hit
GTACATGGGAAGTAAGCAGTGGTAT23840.13429238223081955No Hit
ACATGGTAAGCAGTGGTATCAACGC23260.1310252017906402No Hit
GCGTTGATACCACTGCTTATCACTG23050.1298422571485063No Hit
ACTCTGCGTTGATACCACTGCTTCC22690.12781435204770533No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT22410.12623709252486015No Hit
TTCCCATGTACTCTGCGTTGATACC22150.1247724943965039No Hit
ATGATAAGCAGTGGTATCAACGCAG22100.12449084091028154No Hit
GCAGAGTACATGGGAAGCAGTGGTA21770.12263192790121398No Hit
GTATCAACGCAGAGTACATGATAAG21340.12020970791970173No Hit
GTATCAACGCAGAGTACATGGGAAA20970.11812547212165629No Hit
GCGTTGATACCACTGCTTACTCTGC20280.11423865401178775No Hit
ACGCAGAGTACATGATAAGCAGTGG19390.10922522195702981No Hit
GCCATGTACTCTGCGTTGATACCAC18810.1059580415168505No Hit
TATCAACGCAGAGTACATGATAAGC18500.10421178990227187No Hit
GCTAAGCAGTGGTATCAACGCAGAG18260.10285985316840457No Hit
TATGTACTCTGCGTTGATACCACTG18220.10263453037942667No Hit
CTCTGCGTTGATACCACTGCTTACC17930.10100094015933701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGT307.743836E-418.9925069
GAACAAA7750.016.2057651
ACAGGGG755.8691512E-815.1952893
CGATAAA704.4327498E-714.9298332
CCTCTCT456.6839124E-414.800721
TTTAGAA456.758663E-414.7790272
GTACGTA400.005225431614.2721241
GAGCAAA602.5313595E-514.2721231
CGTGGGC400.00528739514.24678813
TCTCTGG801.2936471E-714.2455843
AGGTTCA551.9487117E-413.82792619
TCGTGGG500.001502881313.29625312
GAACACT500.001503519613.2955046
TAGTACT655.4677726E-513.150144
AGGGTAA655.4721106E-513.1490295
GTACAGG1600.013.082781
GCGTTTA801.9653216E-613.082781
GTACAAA1403.6379788E-1212.9128731
ATTCTAG751.45546E-512.6863321
CAGGTTC604.06827E-412.67559818
Produced by FastQC (version 0.11.5)