FastQCFastQC Report
Wed 25 May 2016
SRR1294523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775231
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC581023.2729261712982702No Hit
ACCATGTACTCTGCGTTGATACCAC419572.3634670642862816No Hit
GTACATGGTAAGCAGTGGTATCAAC395702.2290056899637287No Hit
CATGTACTCTGCGTTGATACCACTG302871.7060878274433016No Hit
GTGGTAAGCAGTGGTATCAACGCAG287291.6183246011364154No Hit
GGTAAGCAGTGGTATCAACGCAGAG273731.5419401756729125No Hit
CCATGTACTCTGCGTTGATACCACT198981.1208682137704895No Hit
GTATCAACGCAGAGTACATGGGAAG196101.1046449729640817No Hit
ACTCTGCGTTGATACCACTGCTTAC182641.0288238544730235No Hit
GTACTCTGCGTTGATACCACTGCTT164630.9273722687357309No Hit
GCAGTGGTATCAACGCAGAGTACAT153470.8645072106109007No Hit
GGTATCAACGCAGAGTACATGGGAA151290.8522271186116059No Hit
GAGTACATGGTAAGCAGTGGTATCA150890.8499738907218273No Hit
GTACATGGGAAGCAGTGGTAAGCAG148270.8352152480437757No Hit
TATCAACGCAGAGTACATGGGAAGC133680.7530287607640921No Hit
GCTTACCATGTACTCTGCGTTGATA131870.7428329045628428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA131580.7411993143427531No Hit
GCGTTGATACCACTGCTTACCATGT128680.7248634121418565No Hit
AAGCAGTGGTATCAACGCAGAGTAC124890.7035140778862019No Hit
GTACATGGGAAGCAGTGGTATCAAC122260.6886991045109059No Hit
GCTTACCACTGCTTCCCATGTACTC121690.685488254767971No Hit
GTATCAACGCAGAGTACTTTTTTTT119440.672813847887965No Hit
GTGGTATCAACGCAGAGTACATGGG116490.656196292200846No Hit
CTGCTTACCATGTACTCTGCGTTGA113460.6391280909357712No Hit
GCGTTGATACCACTGCTTACCACTG112090.6314107854132787No Hit
GTACTTTTTTTTTTTTTTTTTTTTT106910.6022314842406425No Hit
GTATCAACGCAGAGTACATGGTAAG102100.5751364188660518No Hit
GTACATGATAAGCAGTGGTATCAAC101870.573840812829429No Hit
ATGGTAAGCAGTGGTATCAACGCAG100060.5636449566281797No Hit
ATCATGTACTCTGCGTTGATACCAC97510.5492806288308395No Hit
CAGTGGTATCAACGCAGAGTACATG93710.5278749638779403No Hit
TATCAACGCAGAGTACATGGTAAGC91280.5141866044475338No Hit
ACGCAGAGTACATGGTAAGCAGTGG89950.5066946217140192No Hit
TATCAACGCAGAGTACTTTTTTTTT86690.4883308144123215No Hit
ACGCAGAGTACATGGGAAGCAGTGG82420.46427760668893236No Hit
GTGATAAGCAGTGGTATCAACGCAG79670.4487866649467027No Hit
GGTATCAACGCAGAGTACTTTTTTT77900.4388161315344313No Hit
GTACATAAGCAGTGGTATCAACGCA73240.4125660266185076No Hit
GGTATCAACGCAGAGTACATGGTAA70000.394314880711299No Hit
GAGTACATGGGAAGCAGTGGTAAGC65730.37026167298790974No Hit
TACCACTGCTTACCATGTACTCTGC63630.35843222656657076No Hit
GAGTACTTTTTTTTTTTTTTTTTTT61550.3467154415397208No Hit
GTGGTATCAACGCAGAGTACATGGT60710.34198366297118515No Hit
GAGTACATGGGAAGCAGTGGTATCA54680.308016252532769No Hit
GAGTAAGCAGTGGTATCAACGCAGA54620.3076782683493021No Hit
AAAAAGTACTCTGCGTTGATACCAC52020.2930322870657396No Hit
GATAAGCAGTGGTATCAACGCAGAG51090.28779353222200377No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49610.279456589029822No Hit
GCAGAGTACATGGTAAGCAGTGGTA49580.27928759693808863No Hit
GTAAGCAGTGGTATCAACGCAGAGT49340.27793566020422134No Hit
GCTTCCCATGTACTCTGCGTTGATA48420.27275323605772994No Hit
TGGTAAGCAGTGGTATCAACGCAGA46010.25917753802181237No Hit
ACTCTGCGTTGATACCACTGCTTAT45350.2554597120036773No Hit
GCTTATGTACTCTGCGTTGATACCA43650.24588349347211716No Hit
AAAGTACTCTGCGTTGATACCACTG43540.24526385580242796No Hit
CTTCCCATGTACTCTGCGTTGATAC42010.23664525912402384No Hit
GAGTACATGATAAGCAGTGGTATCA40690.22920960708775365No Hit
GTGGTATCAACGCAGAGTACTTTTT40360.2273506940786861No Hit
CTGCTTACCACTGCTTCCCATGTAC39640.22329488387708415No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT39480.2223935927211726No Hit
GCGTTGATACCACTGCTTCCCATGT36900.20786027283209904No Hit
GTTGATACCACTGCTTACCATGTAC36760.20707164307067644No Hit
GTACATGGTAAGCAGTGGTAAGCAG36040.2030158328690745No Hit
GATACCACTGCTTACCATGTACTCT35960.20256518729111872No Hit
GCTTATCATGTACTCTGCGTTGATA35120.19783340872258315No Hit
CTGCTTCCCATGTACTCTGCGTTGA33650.18955279622764587No Hit
ATACCACTGCTTACCATGTACTCTG31960.18003290839333022No Hit
CCACTGCTTACCATGTACTCTGCGT31510.17749802701732903No Hit
GCAGTGGTATCAACGCAGAGTACTT31100.1751884684303057No Hit
CATGGTAAGCAGTGGTATCAACGCA30830.17366753960470496No Hit
GCGTTGATACCACTGCTTATCATGT29940.168654107549947No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA29630.16690785593536842No Hit
ATCAACGCAGAGTACATGGGAAGCA29080.16380966758692247No Hit
GCTTACTCTGCGTTGATACCACTGC29010.16341535270621121No Hit
GTTGATACCACTGCTTACCACTGCT28480.16042982575225423No Hit
TGATACCACTGCTTACCATGTACTC27880.15704998391758593No Hit
GCAGTGGTAAGCAGTGGTATCAACG27820.1567119997341191No Hit
GTACATGGGTAAGCAGTGGTATCAA27560.15524740160576286No Hit
AAGCAGTGGTAAGCAGTGGTATCAA27360.15412078766087342No Hit
CCACTGCTTCCCATGTACTCTGCGT27290.15372647278016213No Hit
ACATGGTAAGCAGTGGTATCAACGC27290.15372647278016213No Hit
GCTTACCACTGCTTACCATGTACTC26440.14893836351438208No Hit
GTACATGGGAAGTAAGCAGTGGTAT26250.14786808026673712No Hit
GTACATGGGAAGCAGTGATAAGCAG26240.14781174956949264No Hit
CTTATGTACTCTGCGTTGATACCAC26070.14685412771633663No Hit
TACCACTGCTTACCACTGCTTCCCA26000.1464598128356253No Hit
TACCACTGCTTCCCATGTACTCTGC25830.1455021909824693No Hit
GGTAAGCAGTGGTAAGCAGTGGTAT25400.14307997100095707No Hit
GCTAAGCAGTGGTATCAACGCAGAG25370.14291097890922363No Hit
GCTTATCACTGCTTCCCATGTACTC24810.13975645986353324No Hit
ACTCTGCGTTGATACCACTGCTTCC24300.13688359430406521No Hit
GCGTTGATACCACTGCTTATCACTG23870.13446137432255295No Hit
ATGATAAGCAGTGGTATCAACGCAG23690.13344742177215246No Hit
GCAGAGTACATGGGAAGCAGTGGTA22930.12916628878157266No Hit
CTGCTAAGCAGTGGTATCAACGCAG22550.12702572228628276No Hit
TTCCCATGTACTCTGCGTTGATACC22260.12539213206619307No Hit
ACGCAGAGTACATGATAAGCAGTGG21860.12313890417641424No Hit
GTATCAACGCAGAGTACATGATAAG21720.12235027441499162No Hit
GTATCAACGCAGAGTACATGGGAAA21370.12037870001143512No Hit
GCGTTGATACCACTGCTTACTCTGC21240.11964640094725701No Hit
GCAGAGTACTTTTTTTTTTTTTTTT21230.11959007025001253No Hit
GCCATGTACTCTGCGTTGATACCAC19850.1118164340302755No Hit
TATCAACGCAGAGTACATGATAAGC19480.10973219823223006No Hit
TATGTACTCTGCGTTGATACCACTG19220.10826760010387379No Hit
ATGTACTCTGCGTTGATACCACTGC19020.10714098615898439No Hit
GTACATGGCAAGCAGTGGTATCAAC18930.10663400988378413No Hit
CATGATAAGCAGTGGTATCAACGCA18920.10657767918653967No Hit
CTCTGCGTTGATACCACTGCTTACC18760.10567638803062812No Hit
GCAGTAAGCAGTGGTATCAACGCAG18510.10426812059951633No Hit
TCCATGTACTCTGCGTTGATACCAC18340.10331049874636032No Hit
GTACATGGGAAGCAATGGTAAGCAG17930.10100094015933701No Hit
CTGCTTACTCTGCGTTGATACCACT17930.10100094015933701No Hit
GTACATAGTAAGCAGTGGTATCAAC17860.10060662527862571No Hit
CAGTGGTATCAACGCAGAGTACTTT17820.10038130248964783No Hit
ATCAACGCAGAGTACATGGTAAGCA17800.10026864109515887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG359.8864824E-519.0135331
CAACGTT651.91847E-716.0734211
AACGTTG601.4704347E-615.82988312
GAACAAA3700.015.1594381
CTGTATC456.748187E-414.7820524
ACTATCG653.3804135E-614.612211
GCTTAAT400.005254615614.2601491
CGCAAAA602.5616704E-514.2541232
TGTATCG400.005269375614.2541225
CTGGTTC707.2422736E-613.5753544
TTCACTA707.271843E-613.578
CCAAGAA1205.0931703E-1113.4622272
ACCAAGA500.00149166913.3094731
CCCAAGA801.9839335E-613.0718031
CACAACG1403.6379788E-1212.89149959
CTGCTAA4450.012.8181111
ATACAGT604.0825625E-412.6703316
TACTGGT905.387483E-712.670332
CAAGAAC1352.7284841E-1112.670333
CATGAAC1700.012.2976733
Produced by FastQC (version 0.11.5)