FastQCFastQC Report
Wed 25 May 2016
SRR1294522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634529
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC92480.3510304878025636No Hit
ACCATGTACTCTGCGTTGATACCAC82840.31443950702383616No Hit
GTACATGGTAAGCAGTGGTATCAAC79250.30081278285416485No Hit
GTGGTAAGCAGTGGTATCAACGCAG55100.20914554366264332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52880.2007189899978326No Hit
GTGGTATCAACGCAGAGTACATGGG52620.1997320963253773No Hit
CATGTACTCTGCGTTGATACCACTG50090.1901288617434084No Hit
ATTCCATTCCATTCCATTCCATTCC46150.17517362686081647No Hit
GGTAAGCAGTGGTATCAACGCAGAG43950.166822988093887No Hit
GAATGGAATGGAATGGAATGGAATG38050.1444280932189397No Hit
CCATGTACTCTGCGTTGATACCACT37180.1411257951611085No Hit
GTATCAACGCAGAGTACTTTTTTTT34550.13114298608973368No Hit
GTATCAACGCAGAGTACATGGGAAG31290.1187688577351018No Hit
ACTCTGCGTTGATACCACTGCTTAC30940.11744034702218119No Hit
GGTATCAACGCAGAGTACATGGGAA30780.11683302783913178No Hit
GCTTACCATGTACTCTGCGTTGATA29800.1131131978429541No Hit
GAGTACATGGTAAGCAGTGGTATCA29630.11246792121096408No Hit
GCAGTGGTATCAACGCAGAGTACAT27580.10468664417814341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT400.00528866114.2466396
CTCCGTT658.041282E-411.6897737
GTGTACG600.005829832511.0954641
CGTATCA700.001495646410.85396510
CCGTACC856.609346E-410.0566434
CTGTCGC2002.5465852E-119.9724599
CTACACT3100.09.497944
TATTCCG1201.7064E-59.4975815
GTATTAG3850.09.3868861
GTGTAGA3000.09.1933841
GTACTGT2700.09.1459916
CCAGTAC2304.1836756E-119.0846413
TAGACAG3700.08.9841975
GTCTAAC1502.6632388E-68.8763711
AGACTGC2252.6375346E-108.8645756
GTCTAGA2605.456968E-128.7788291
GTATAGA3250.08.7788291
TATACTG6400.08.7555815
CCTACAC2401.0186341E-108.7061163
GTACTAG1651.0340646E-68.6458161
Produced by FastQC (version 0.11.5)