FastQCFastQC Report
Wed 25 May 2016
SRR1294522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634529
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116810.4433809610750157No Hit
CCCATGTACTCTGCGTTGATACCAC96640.3668207865618484No Hit
ACCATGTACTCTGCGTTGATACCAC87670.3327729548621405No Hit
GTACATGGTAAGCAGTGGTATCAAC82460.3129971239640938No Hit
GGTATCAACGCAGAGTACTTTTTTT80100.30403916601411485No Hit
TATCAACGCAGAGTACTTTTTTTTT66530.2525309078017361No Hit
GTGGTAAGCAGTGGTATCAACGCAG61560.23366605567826357No Hit
GTGGTATCAACGCAGAGTACATGGG53900.20459064978977265No Hit
CATGTACTCTGCGTTGATACCACTG51910.19703711745059554No Hit
GGTAAGCAGTGGTATCAACGCAGAG47170.17904528665275654No Hit
ATTCCATTCCATTCCATTCCATTCC47040.1785518398165289No Hit
CCATGTACTCTGCGTTGATACCACT38760.1471230720937215No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37560.14256817822085088No Hit
GAATGGAATGGAATGGAATGGAATG36170.13729209281810906No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34310.13023200731515955No Hit
GTATCAACGCAGAGTACATGGGAAG34150.12962468813211014No Hit
ACTCTGCGTTGATACCACTGCTTAC33680.1278406880319025No Hit
GGTATCAACGCAGAGTACATGGGAA32220.12229890048657654No Hit
GAGTACATGGTAAGCAGTGGTATCA31980.12138792171200241No Hit
GCAGTGGTATCAACGCAGAGTACAT30890.11725055977747825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA30650.11633958100290412No Hit
GCTTACCATGTACTCTGCGTTGATA30410.11542860222833No Hit
GTACATGGGAAGCAGTGGTAAGCAG26980.1024091972417081No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26460.10043540989679749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGG500.001499578213.3005317
TATAACG550.003065446912.0929992
TCGCTCT1657.2759576E-1211.5171424
ACACCGT752.0715286E-411.4019726
GTCTCGC1553.6343408E-910.424721
TAACGCA750.002644819210.1350864
CTAGCAC2350.010.108133
CTCGCTC1803.929017E-1010.0295113
GTCTAAT3300.09.7929191
CGCTCTG1651.0200893E-89.7895715
CCTACAC1951.6370905E-109.7452743
GTATTAC2401.8189894E-129.5048911
TAACCCG1304.2315733E-69.5016435
TCAGGAC2252.7284841E-119.2904963
GTCTTGC4450.09.1845021
GTCCTAG2201.6552804E-109.0728511
ATTAGAC2106.657501E-109.0491833
CGCCCGC850.0074409738.94068510
CAGGACA4150.08.9292554
CCAGGAC3300.08.9257853
Produced by FastQC (version 0.11.5)