FastQCFastQC Report
Wed 25 May 2016
SRR1294521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687319
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC348351.2962733490143894No Hit
ACCATGTACTCTGCGTTGATACCAC242360.9018653907481768No Hit
GTACATGGTAAGCAGTGGTATCAAC229250.8530807098078047No Hit
GTGGTAAGCAGTGGTATCAACGCAG174930.6509461660487645No Hit
CATGTACTCTGCGTTGATACCACTG168400.6266468550998225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA152020.5656939127807306No Hit
GGTAAGCAGTGGTATCAACGCAGAG145690.5421388380017408No Hit
CCATGTACTCTGCGTTGATACCACT114550.42626126634017025No Hit
GTATCAACGCAGAGTACATGGGAAG112330.41800024485369985No Hit
ACTCTGCGTTGATACCACTGCTTAC104570.38912388145955135No Hit
GTACATGGGAAGCAGTGGTAAGCAG98680.3672061262544566No Hit
GTACTCTGCGTTGATACCACTGCTT97590.36315003912821664No Hit
GCAGTGGTATCAACGCAGAGTACAT89890.3344969465850537No Hit
GTGGTATCAACGCAGAGTACATGGG86580.3221798379723434No Hit
GGTATCAACGCAGAGTACATGGGAA86530.32199377892985537No Hit
GAGTACATGGTAAGCAGTGGTATCA85280.3173423028676536No Hit
GCTTACCACTGCTTCCCATGTACTC82260.3061043367013741No Hit
GCTTACCATGTACTCTGCGTTGATA78970.29386165170565903No Hit
GCGTTGATACCACTGCTTACCATGT76880.2860843837296577No Hit
GTGATAAGCAGTGGTATCAACGCAG74150.27592556000980906No Hit
GTACATGGGAAGCAGTGGTATCAAC71450.2658783717154532No Hit
TATCAACGCAGAGTACATGGGAAGC71320.2653946182049842No Hit
GCGTTGATACCACTGCTTACCACTG67090.24965402321049346No Hit
CTGCTTACCATGTACTCTGCGTTGA64880.2414302135325207No Hit
AAGCAGTGGTATCAACGCAGAGTAC64190.23886259874618532No Hit
GTATCAACGCAGAGTACATGGTAAG61840.23011782374924597No Hit
GTACATAAGCAGTGGTATCAACGCA58940.2193263992849379No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA56230.20924199918208444No Hit
CAGTGGTATCAACGCAGAGTACATG52780.19640392525040756No Hit
TATCAACGCAGAGTACATGGTAAGC49070.1825983442977927No Hit
ATGGTAAGCAGTGGTATCAACGCAG48740.18137035461737144No Hit
ACGCAGAGTACATGGTAAGCAGTGG47940.1783934099375623No Hit
GTACATGATAAGCAGTGGTATCAAC47610.17716542025714105No Hit
ACGCAGAGTACATGGGAAGCAGTGG45640.16983469398311105No Hit
ATCATGTACTCTGCGTTGATACCAC41920.15599190122199857No Hit
GAGTACATGGGAAGCAGTGGTAAGC40340.15011243547937553No Hit
GGTATCAACGCAGAGTACATGGTAA37310.13883725750459844No Hit
GCTTATGTACTCTGCGTTGATACCA36830.13705109069671298No Hit
TACCACTGCTTACCATGTACTCTGC36310.13511607665483702No Hit
GTATCAACGCAGAGTACTTTTTTTT35590.1324368264430088No Hit
GAGTAAGCAGTGGTATCAACGCAGA34110.12692947878536193No Hit
GTGGTATCAACGCAGAGTACATGGT33960.1263713016578977No Hit
GCTTCCCATGTACTCTGCGTTGATA32830.1221663672976673No Hit
GATAAGCAGTGGTATCAACGCAGAG32520.12101280123424127No Hit
ACTCTGCGTTGATACCACTGCTTAT31450.11703113772499654No Hit
AAAAAGTACTCTGCGTTGATACCAC30980.11528218272560868No Hit
GAGTACATGGGAAGCAGTGGTATCA30650.11405419304518741No Hit
GTAAGCAGTGGTATCAACGCAGAGT27160.10106727187952007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAC400.005288672614.24664913
GTCTACG550.003041156312.1058661
TAAGACT1302.6266207E-911.6899944
GACTAAT855.259325E-511.1902961
CCGCGTG855.3377327E-511.1740519
GTCTAGA1451.195076E-911.1517091
TAGGCGT600.0058862111.08114054
CTAATTC1053.4769128E-610.8549943
GGCCTAA1158.6843283E-710.7524151
TGAACGG803.7827602E-410.6843910
TATTCCG909.5373995E-510.5536635
GTATTAA3300.010.3764571
GTATAGG1201.5007845E-610.3043981
CTACACT2053.6379788E-1210.1931054
ACACCGT750.002652587610.1315176
CTAAGAC856.608286E-410.0568333
GAACAAA6150.010.0530711
GCTTATG9050.09.9847131
GTCCTAG1151.0120517E-59.9253061
TAGACTG1752.582965E-99.7696765
Produced by FastQC (version 0.11.5)