FastQCFastQC Report
Wed 25 May 2016
SRR1294521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687319
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC366791.36489192388399No Hit
ACCATGTACTCTGCGTTGATACCAC250220.9311138722273016No Hit
GTACATGGTAAGCAGTGGTATCAAC236200.8789429167136465No Hit
GTGGTAAGCAGTGGTATCAACGCAG193630.720532247939303No Hit
CATGTACTCTGCGTTGATACCACTG175460.6529183918991381No Hit
GGTAAGCAGTGGTATCAACGCAGAG160210.5961703839402765No Hit
CCATGTACTCTGCGTTGATACCACT121580.45242116771399304No Hit
GTATCAACGCAGAGTACTTTTTTTT119200.4435647572915608No Hit
GTATCAACGCAGAGTACATGGGAAG117150.4359363365495499No Hit
ACTCTGCGTTGATACCACTGCTTAC110250.4102601886861961No Hit
GTACATGGGAAGCAGTGGTAAGCAG102180.3802302592286215No Hit
GTACTCTGCGTTGATACCACTGCTT96660.35968934093793853No Hit
GCAGTGGTATCAACGCAGAGTACAT96150.3577915387045602No Hit
GAGTACATGGTAAGCAGTGGTATCA90250.3358365716909678No Hit
GTGGTATCAACGCAGAGTACATGGG89780.33408761669158No Hit
GGTATCAACGCAGAGTACATGGGAA88870.3307013421182971No Hit
GCTTACCACTGCTTCCCATGTACTC85100.3166724903146965No Hit
GCTTACCATGTACTCTGCGTTGATA81200.302159885000627No Hit
GTGATAAGCAGTGGTATCAACGCAG80770.30055977723522964No Hit
GCGTTGATACCACTGCTTACCATGT79680.29650369010898964No Hit
TATCAACGCAGAGTACATGGGAAGC77010.28656813724012664No Hit
GTACATGGGAAGCAGTGGTATCAAC74890.27867923383863247No Hit
GGTATCAACGCAGAGTACTTTTTTT74520.27730239692422076No Hit
TATCAACGCAGAGTACTTTTTTTTT74400.27685585522224937No Hit
AAGCAGTGGTATCAACGCAGAGTAC74200.27611161905229714No Hit
GCGTTGATACCACTGCTTACCACTG70510.26238046171667745No Hit
CTGCTTACCATGTACTCTGCGTTGA68010.25307750959227393No Hit
GTATCAACGCAGAGTACATGGTAAG61350.22829444513286287No Hit
CAGTGGTATCAACGCAGAGTACATG59270.22055438896535917No Hit
GTACATAAGCAGTGGTATCAACGCA58860.219028704816957No Hit
GTACTTTTTTTTTTTTTTTTTTTTT58740.2185821631149856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA55400.20615341907678245No Hit
ATGGTAAGCAGTGGTATCAACGCAG55080.2049626412048588No Hit
ACGCAGAGTACATGGTAAGCAGTGG51780.19268274440064614No Hit
ACGCAGAGTACATGGGAAGCAGTGG50420.1876219384449706No Hit
TATCAACGCAGAGTACATGGTAAGC49520.18427287568018536No Hit
GTACATGATAAGCAGTGGTATCAAC47340.17616070142770546No Hit
GAGTACATGGGAAGCAGTGGTAAGC44790.16667169026081383No Hit
ATCATGTACTCTGCGTTGATACCAC43800.16298772121955005No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39900.14847511590548051No Hit
GGTATCAACGCAGAGTACATGGTAA39470.1468750081400831No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37910.1410699660144553No Hit
TACCACTGCTTACCATGTACTCTGC37810.14069784792947915No Hit
GCTTATGTACTCTGCGTTGATACCA37040.13783253867516287No Hit
GATAAGCAGTGGTATCAACGCAGAG36260.13493001761234896No Hit
GAGTAAGCAGTGGTATCAACGCAGA35850.13340433346394678No Hit
GTGGTATCAACGCAGAGTACATGGT35350.13154374303906607No Hit
GCTTCCCATGTACTCTGCGTTGATA34200.12726438506184046No Hit
ACTCTGCGTTGATACCACTGCTTAT33420.12436186399902653No Hit
AAAAAGTACTCTGCGTTGATACCAC32700.12168261378719832No Hit
GAGTACATGGGAAGCAGTGGTATCA32200.11982202336231762No Hit
GTAAGCAGTGGTATCAACGCAGAGT28700.10679789038815266No Hit
AAAGTACTCTGCGTTGATACCACTG27880.10374652209134828No Hit
CTGCTTACCACTGCTTCCCATGTAC27330.10169987262397952No Hit
TGGTAAGCAGTGGTATCAACGCAGA27290.10155102538998906No Hit
GTGGTATCAACGCAGAGTACTTTTT27040.1006207301775487No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAG400.005285850714.24779515
ACCGCGG658.0334285E-411.6911518
CCCGTAT907.4743366E-611.6099638
ATCGGAT1053.4758868E-610.85526216
GTGTAGC2400.010.2983141
TCTAGTA750.002643464110.1357262
CAGGACC3400.010.0610114
CCTAGAC1252.5644676E-69.8821493
GTACTAA1554.026333E-89.8127851
GAACAAA3900.09.7498841
GAGTATA2850.09.6729111
GCTTATG8750.09.6690981
GTTCTAG2106.548362E-119.5061371
GTGTAGG1606.5743734E-89.5061361
CTTAAGG2106.730261E-119.5020673
TAGAACA2201.6370905E-119.5020674
GTTAGCC800.00450916039.5020673
TAAGGTG2201.6370905E-119.5020675
CTAGGAC800.00450916039.5020673
GACAGTA1201.6999218E-59.5006517
Produced by FastQC (version 0.11.5)