FastQCFastQC Report
Wed 25 May 2016
SRR1294520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804622
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC73000.40451684618717937No Hit
GTACATGGTAAGCAGTGGTATCAAC55730.3088181347672809No Hit
ACCATGTACTCTGCGTTGATACCAC53020.2938011395184144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA49920.2766230268721095No Hit
GTGGTAAGCAGTGGTATCAACGCAG42150.23356691872314533No Hit
CATGTACTCTGCGTTGATACCACTG37520.20791057628689No Hit
GTGGTATCAACGCAGAGTACATGGG36700.20336668842560937No Hit
GGTAAGCAGTGGTATCAACGCAGAG32720.18131220831841793No Hit
GTATCAACGCAGAGTACTTTTTTTT26140.1448502788949708No Hit
CCATGTACTCTGCGTTGATACCACT24760.13720324810403509No Hit
GTATCAACGCAGAGTACATGGGAAG23890.1323822939097495No Hit
GGTATCAACGCAGAGTACATGGGAA22250.12329451818718824No Hit
ACTCTGCGTTGATACCACTGCTTAC22170.12285121205438036No Hit
GCAGTGGTATCAACGCAGAGTACAT21230.11764236499388792No Hit
GAGTACATGGTAAGCAGTGGTATCA21150.11719905886108005No Hit
GTACATGGGAAGCAGTGGTAAGCAG20440.11326471693241022No Hit
GCTTACCATGTACTCTGCGTTGATA20180.11182397200078466No Hit
GTACTCTGCGTTGATACCACTGCTT19790.1096628546033463No Hit
GGTATCAACGCAGAGTACTTTTTTT18460.1022928901454155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG400.00528511114.2477345
GTACCGT500.001501822113.2975166
CGTGTTA550.003074344512.08798210
CGTGGGG951.363238E-510.99794713
ACTGATC1502.1409505E-910.76435858
GTTATAC1355.6961653E-810.55446153
TAGTACT1355.6961653E-810.55446154
TAGCACT2250.010.1322834
TTAGTAC1803.947207E-1010.026743
CCGTGTT1054.114885E-59.9502489
TCGTGGG1054.114885E-59.95024812
TAGGACT1856.493792E-109.7557474
TATACTG4100.09.730165
GTTCTAG1804.094545E-99.5114071
TTAGACT1804.1873136E-99.4990174
ACATAAG5500.09.4990163
GGACAGT2106.730261E-119.4982276
GGACTGA1502.6548878E-79.4982266
CTAGGAC1856.706614E-99.2422863
AGGACCA1356.860031E-69.1466935
Produced by FastQC (version 0.11.5)