FastQCFastQC Report
Wed 25 May 2016
SRR1294520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804622
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT91180.5052581648677673No Hit
CCCATGTACTCTGCGTTGATACCAC77470.429286576357819No Hit
GGTATCAACGCAGAGTACTTTTTTT64010.3547003195128952No Hit
GTACATGGTAAGCAGTGGTATCAAC58870.3262179004799897No Hit
ACCATGTACTCTGCGTTGATACCAC58600.3247217422817632No Hit
TATCAACGCAGAGTACTTTTTTTTT54820.3037755275065914No Hit
GTGGTAAGCAGTGGTATCAACGCAG48260.26742442461634625No Hit
CATGTACTCTGCGTTGATACCACTG38680.21433851521260408No Hit
GTGGTATCAACGCAGAGTACATGGG38610.2139506223463972No Hit
GGTAAGCAGTGGTATCAACGCAGAG36550.2025354894265946No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31380.17388683059388615No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28590.15842652921221176No Hit
CCATGTACTCTGCGTTGATACCACT27700.15349474848472422No Hit
GTATCAACGCAGAGTACATGGGAAG27060.1499482994222613No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA24480.13565167663920755No Hit
GGTATCAACGCAGAGTACATGGGAA23650.1310523755113259No Hit
ACTCTGCGTTGATACCACTGCTTAC23380.12955621731309935No Hit
GCAGTGGTATCAACGCAGAGTACAT22540.12490150291861676No Hit
GAGTACATGGTAAGCAGTGGTATCA22070.12229707938837053No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21670.1200805487243312No Hit
GCTTACCATGTACTCTGCGTTGATA21040.11658951292846922No Hit
GTACATGGGAAGCAGTGGTAAGCAG20830.11542583432984858No Hit
GTACTCTGCGTTGATACCACTGCTT19120.1059501657410804No Hit
GCTTACCACTGCTTCCCATGTACTC18990.1052297932752676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATTG1201.2736382E-711.0844987
GTCTTAT2053.6379788E-1210.2018481
GTATAGG1502.4196197E-810.1400181
CTGTCGC1502.4521796E-810.1313099
ATTATAC3400.010.0621123
GTCCTAC1054.075559E-59.9589471
TATACTG4250.09.838515
TGTCGCC1554.075264E-89.80449310
GTCCATA1401.0489384E-69.50626851
GCTTAAC1106.7871646E-59.5062681
TCTGCAC2303.6379788E-129.5031053
GGACTTA800.0045038869.5031056
GCTATAC800.0045038869.5031053
GTAGGAC800.0045038869.5031053
CGGTAAG800.0045038869.5031056
ACGGTAA900.0011103729.5031055
CGTGCCT800.00452349439.49810210
TAGGACA1651.0599615E-79.2151324
GTCGCCC1356.86358E-69.1463211
TAGGACC1252.7431703E-59.1229814
Produced by FastQC (version 0.11.5)