FastQCFastQC Report
Wed 25 May 2016
SRR1294519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118665
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC283071.3360771995572684No Hit
ACCATGTACTCTGCGTTGATACCAC207090.9774551427431897No Hit
GTACATGGTAAGCAGTGGTATCAAC196820.92898122166553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA164740.7775651176566376No Hit
GTGGTAAGCAGTGGTATCAACGCAG162940.7690692015962882No Hit
CATGTACTCTGCGTTGATACCACTG149820.7071434134230754No Hit
GGTAAGCAGTGGTATCAACGCAGAG128980.6087795852576977No Hit
CCATGTACTCTGCGTTGATACCACT96440.45519230270004934No Hit
GTATCAACGCAGAGTACATGGGAAG90080.425173399286815No Hit
ACTCTGCGTTGATACCACTGCTTAC89210.4210670398576462No Hit
GTACATGGGAAGCAGTGGTAAGCAG85310.4026592217268894No Hit
GTACTCTGCGTTGATACCACTGCTT82620.389962547170034No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA79250.3740563043237133No Hit
GCAGTGGTATCAACGCAGAGTACAT77190.36433320038798017No Hit
GAGTACATGGTAAGCAGTGGTATCA73900.34880455381100833No Hit
GTACATGATAAGCAGTGGTATCAAC73020.3446509948481709No Hit
ATCATGTACTCTGCGTTGATACCAC70400.33228471702699575No Hit
GCTTACCATGTACTCTGCGTTGATA70130.3310103296179434No Hit
GCTTACCACTGCTTCCCATGTACTC67240.31736966438771586No Hit
GCGTTGATACCACTGCTTACCATGT65960.3113281240781341No Hit
GTACATGGGAAGCAGTGGTATCAAC65650.3098649385344073No Hit
GGTATCAACGCAGAGTACATGGGAA64010.30212421501275566No Hit
GTGGTATCAACGCAGAGTACATGGG63350.2990090457906276No Hit
GCGTTGATACCACTGCTTACCACTG62720.2960354751695053No Hit
GTACATAAGCAGTGGTATCAACGCA58780.27743885890407405No Hit
TATCAACGCAGAGTACATGGGAAGC58410.27569247615833553No Hit
CTGCTTACCATGTACTCTGCGTTGA58130.27437088921561453No Hit
AAGCAGTGGTATCAACGCAGAGTAC56450.2664413675592885No Hit
GTATCAACGCAGAGTACATGGTAAG54650.2579454514989392No Hit
ATGGTAAGCAGTGGTATCAACGCAG46370.21886423762133228No Hit
CAGTGGTATCAACGCAGAGTACATG44400.20956592948861663No Hit
TATCAACGCAGAGTACATGGTAAGC43000.20295799477501164No Hit
ACGCAGAGTACATGGTAAGCAGTGG41060.19380128524330179No Hit
GCTTATGTACTCTGCGTTGATACCA39010.18412538084123728No Hit
ACGCAGAGTACATGGGAAGCAGTGG37430.17666785452159733No Hit
GAGTACATGGGAAGCAGTGGTAAGC35290.16656715431651536No Hit
GGTATCAACGCAGAGTACATGGTAA33660.15887363032853236No Hit
GTGATAAGCAGTGGTATCAACGCAG32870.15514486716871237No Hit
TACCACTGCTTACCATGTACTCTGC31760.14990571893149696No Hit
GTATCAACGCAGAGTACTTTTTTTT31200.14726254504605496No Hit
GTGGTATCAACGCAGAGTACATGGT30120.14216499540984534No Hit
GTACATGGTAAGCAGTGGTAAGCAG28830.136076255566595No Hit
GCTTCCCATGTACTCTGCGTTGATA28240.13329148308014716No Hit
GAGTACATGGGAAGCAGTGGTATCA27250.12861872924695503No Hit
AAAAAGTACTCTGCGTTGATACCAC27210.12842993111228063No Hit
GAGTACATGATAAGCAGTGGTATCA26280.12404037448110013No Hit
GCTTATCATGTACTCTGCGTTGATA24950.11776283650317536No Hit
GAGTAAGCAGTGGTATCAACGCAGA24400.11516686215140196No Hit
GATAAGCAGTGGTATCAACGCAGAG24330.11483646541572169No Hit
ACTCTGCGTTGATACCACTGCTTAT24070.11360927754033791No Hit
AAAGTACTCTGCGTTGATACCACTG23620.11148529852525056No Hit
GCTTACCACTGCTTACCATGTACTC23470.11077730552022146No Hit
GCAGAGTACATGGTAAGCAGTGGTA23140.10921972090915742No Hit
GCGTTGATACCACTGCTTATCATGT21840.10308378153223846No Hit
TGGTAAGCAGTGGTATCAACGCAGA21570.10180939412318606No Hit
GTAAGCAGTGGTATCAACGCAGAGT21490.10143179785383721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG456.775432E-414.7744185
GAACAAA7050.012.550991
GTATTAG1406.6393113E-1011.5532741
GTTCTAG752.0453904E-411.4173531
CACAACG2150.011.0437399
GTCCTAA951.3379804E-511.0167441
GTACAAA2450.010.873671
TAGACTA700.001494621410.8546745
GCTTATG8700.010.8267991
TAAACAG2650.010.7522725
GCGAAAA1605.311449E-1010.7037691
CAAGATA803.7798248E-410.685074
CGCAAAA1252.2363929E-710.6416012
CTACACT3600.010.5531564
GCTACAC3250.010.5206843
AGGTGCG1553.6798156E-910.41649311
GTACTAA1105.938624E-610.3794121
GATTAAT1201.4947491E-610.3073321
GCCTATA750.002615042310.1487581
TATGGAC750.002653250210.1310293
Produced by FastQC (version 0.11.5)