FastQCFastQC Report
Wed 25 May 2016
SRR1294519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118665
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC299571.4139564301104706No Hit
ACCATGTACTCTGCGTTGATACCAC218151.0296578269806695No Hit
GTACATGGTAAGCAGTGGTATCAAC211670.9990725291634118No Hit
GTGGTAAGCAGTGGTATCAACGCAG187770.8862656436954404No Hit
CATGTACTCTGCGTTGATACCACTG157260.7422598664725192No Hit
GGTAAGCAGTGGTATCAACGCAGAG146430.6911427715094175No Hit
GTATCAACGCAGAGTACTTTTTTTT111560.5265579976069836No Hit
CCATGTACTCTGCGTTGATACCACT102430.4834648233675451No Hit
GTATCAACGCAGAGTACATGGGAAG100260.47322252456145736No Hit
GTACATGGGAAGCAGTGGTAAGCAG95360.45009475306383967No Hit
ACTCTGCGTTGATACCACTGCTTAC94740.4471683819763861No Hit
GCAGTGGTATCAACGCAGAGTACAT86500.40827596623345364No Hit
GTACTCTGCGTTGATACCACTGCTT83000.39175612944944105No Hit
GAGTACATGGTAAGCAGTGGTATCA80900.3818442273790335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA77520.3658907849990442No Hit
GTACATGATAAGCAGTGGTATCAAC75280.35531808945727616No Hit
ATCATGTACTCTGCGTTGATACCAC73850.3485685561426653No Hit
GGTATCAACGCAGAGTACATGGGAA72300.3412526284240312No Hit
GCTTACCATGTACTCTGCGTTGATA72150.34054463541900204No Hit
GCTTACCACTGCTTCCCATGTACTC70990.3350694895134436No Hit
GTACATGGGAAGCAGTGGTATCAAC70510.3328039118973505No Hit
TATCAACGCAGAGTACTTTTTTTTT69320.3271871673907862No Hit
GTGGTATCAACGCAGAGTACATGGG68250.32213681728824517No Hit
GCGTTGATACCACTGCTTACCATGT67560.3188800494651113No Hit
AAGCAGTGGTATCAACGCAGAGTAC67410.3181720564600822No Hit
GGTATCAACGCAGAGTACTTTTTTT66590.31430169469925634No Hit
TATCAACGCAGAGTACATGGGAAGC65190.30769375998565135No Hit
GTACTTTTTTTTTTTTTTTTTTTTT63820.3012274238730521No Hit
GCGTTGATACCACTGCTTACCACTG62910.2969322663092089No Hit
GTACATAAGCAGTGGTATCAACGCA60830.28711476330613855No Hit
CTGCTTACCATGTACTCTGCGTTGA60540.2857459768297489No Hit
GTATCAACGCAGAGTACATGGTAAG56550.2669133628959746No Hit
ATGGTAAGCAGTGGTATCAACGCAG51340.24232240585463014No Hit
CAGTGGTATCAACGCAGAGTACATG50510.23840484456013578No Hit
ACGCAGAGTACATGGTAAGCAGTGG45320.21390828658612854No Hit
TATCAACGCAGAGTACATGGTAAGC44370.20942433088761084No Hit
ACGCAGAGTACATGGGAAGCAGTGG44200.2086219388152445No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41140.19417888151265064No Hit
GAGTACATGGGAAGCAGTGGTAAGC38990.18403098177390007No Hit
ACGCAGAGTACTTTTTTTTTTTTTT38010.17940542747437654No Hit
GCTTATGTACTCTGCGTTGATACCA37970.17921662933970212No Hit
GTGATAAGCAGTGGTATCAACGCAG37230.1757238638482252No Hit
GGTATCAACGCAGAGTACATGGTAA35500.1675583445235561No Hit
TACCACTGCTTACCATGTACTCTGC34290.16184720094965463No Hit
GTGGTATCAACGCAGAGTACATGGT30860.1456577609013223No Hit
GAGTACATGGGAAGCAGTGGTATCA30230.14268419028020005No Hit
GTACATGGTAAGCAGTGGTAAGCAG29840.14084340846712434No Hit
GCTTCCCATGTACTCTGCGTTGATA29680.14008821592842663No Hit
GAGTACATGATAAGCAGTGGTATCA29470.13909702572138588No Hit
AAAAAGTACTCTGCGTTGATACCAC28670.1353210630278973No Hit
GATAAGCAGTGGTATCAACGCAGAG28080.13253629054144944No Hit
GAGTAAGCAGTGGTATCAACGCAGA26450.12484276655346646No Hit
GCTTATCATGTACTCTGCGTTGATA26280.12404037448110013No Hit
ACTCTGCGTTGATACCACTGCTTAT25920.12234119126903027No Hit
GCAGAGTACATGGTAAGCAGTGGTA24850.11729084116648927No Hit
GTAAGCAGTGGTATCAACGCAGAGT24740.1167716462961346No Hit
TGGTAAGCAGTGGTATCAACGCAGA24270.11455326821371004No Hit
GCTTACCACTGCTTACCATGTACTC24220.11431727054536701No Hit
GTGGTATCAACGCAGAGTACTTTTT23180.10940851904383184No Hit
AAAGTACTCTGCGTTGATACCACTG22900.10808693210111085No Hit
GTGGTAAGCAGTGGTAAGCAGTGGT22770.10747333816341896No Hit
GCGTTGATACCACTGCTTATCATGT22120.10440536847495947No Hit
CTGCTTACCACTGCTTCCCATGTAC21700.10242298806087796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG350.002161272316.296161
GGTCGCG350.002170838616.2853897
ACCGACC456.772051E-414.7753928
AAACCGA400.005270720514.2537546
CCCCTAT655.421591E-513.1622841
GAACAAA3250.011.9923031
TTAGTAC802.8584098E-511.878693
CTTGGAC1657.2759576E-1211.5187293
TACACCG752.0694238E-411.4030045
ATGACGC600.005863279611.0867773
CAGTCTA600.005883921411.0815439
TAAGGCT803.76108E-410.6908214
TAGGACC909.4879695E-510.5588354
GTGTTAC1002.3825784E-510.4567031
GTACAAC1303.8090548E-710.2373321
TTCTAAT750.002641434510.1364812
CCGTCCT750.002651800610.1316979
CTGCTAA3750.09.8863381
TAGAACT1252.5604604E-69.8830694
TAATCCT1356.417213E-79.8549134
Produced by FastQC (version 0.11.5)