FastQCFastQC Report
Wed 25 May 2016
SRR1294518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482957
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC79710.3210285155965246No Hit
GTACATGGTAAGCAGTGGTATCAAC59770.24072104349773274No Hit
ACCATGTACTCTGCGTTGATACCAC58750.2366130384054174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA56000.22553753448005745No Hit
GTGGTAAGCAGTGGTATCAACGCAG44920.18091332230078894No Hit
CATGTACTCTGCGTTGATACCACTG40980.16504514576772777No Hit
GTGGTATCAACGCAGAGTACATGGG38910.15670831190391132No Hit
GGTAAGCAGTGGTATCAACGCAGAG33640.1354836189269488No Hit
CCATGTACTCTGCGTTGATACCACT28160.11341316019568602No Hit
GTATCAACGCAGAGTACATGGGAAG27600.11115778485088547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA402.7671602E-416.6208934
TACGACT500.001502782713.2967144
GTCTTAG1355.5855708E-810.5691271
CGAAACA909.535485E-510.55379915
CTAACAC2251.8189894E-129.7092993
GTCTTGG3650.09.6425181
ATAATAC4800.09.4982273
GCACCGT1002.7631738E-49.4976536
TAGGACC1606.6644134E-89.4976534
TCTGTCG1252.7637647E-59.1175638
CTAATAC2401.0913936E-119.1024683
GACTGTC2106.712071E-109.0453847
GTTCTAG1804.1336534E-88.9837581
TTAGACA2750.08.97964
CCGAGTT850.0074475318.93968914
TGGACCG850.00745245448.9389685
ACACTAG850.00745245448.9389686
CTAGAAC2252.6375346E-108.8650123
TGTCTCG1401.0891499E-58.8190738
GTACTAG1752.575198E-78.6968811
Produced by FastQC (version 0.11.5)