FastQCFastQC Report
Wed 25 May 2016
SRR1294518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482957
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC86060.346602861024174No Hit
GTATCAACGCAGAGTACTTTTTTTT83420.3359703772558284No Hit
ACCATGTACTCTGCGTTGATACCAC62180.25042721239232096No Hit
GTACATGGTAAGCAGTGGTATCAAC61680.24841348440589184No Hit
GGTATCAACGCAGAGTACTTTTTTT57780.23270640611174498No Hit
TATCAACGCAGAGTACTTTTTTTTT50460.2032254283904232No Hit
GTGGTAAGCAGTGGTATCAACGCAG48990.1973050681103217No Hit
CATGTACTCTGCGTTGATACCACTG44470.17910096711300277No Hit
GTGGTATCAACGCAGAGTACATGGG40240.16206482834781272No Hit
GGTAAGCAGTGGTATCAACGCAGAG38520.15513760407449667No Hit
GTATCAACGCAGAGTACATGGGAAG29670.11949461871470186No Hit
CCATGTACTCTGCGTTGATACCACT29670.11949461871470186No Hit
ACTCTGCGTTGATACCACTGCTTAC26710.10757334903504169No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26440.10648593592236999No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26400.10632483768345566No Hit
GGTATCAACGCAGAGTACATGGGAA24930.10040447740335415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCA551.129554E-515.54443212
CTCGCGC550.003070845712.09011410
TAGACAG3700.010.5286675
CCGTCAC856.604775E-410.05735213
TCTATAC2700.09.8533873
GTCTTAC2054.0017767E-119.7373431
TGGACTG4150.09.6159565
GTCTTAG1401.050772E-69.5055021
GACCCGC800.004511259.5014816
AAGACCG900.00111241999.501485
CCTCGCG800.0045187529.4995679
AGACAGT4150.09.3870056
GGACTGT3150.09.3506636
CTAGACA2750.09.3287274
GTATTGA2357.2759576E-129.3032571
CTAGGCT2151.05501385E-109.2805164
TAGGACC1856.682967E-99.2446834
TAAGGCT1554.252779E-79.1949814
CGTCACC1054.5004292E-49.04629614
TAGGCTG3900.09.0142255
Produced by FastQC (version 0.11.5)