FastQCFastQC Report
Wed 25 May 2016
SRR1294517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863722
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC273460.9549111261498149No Hit
ACCATGTACTCTGCGTTGATACCAC169130.5905950368087405No Hit
GTGGTAAGCAGTGGTATCAACGCAG160370.5600054753918153No Hit
GTACATGGTAAGCAGTGGTATCAAC154910.5409393788922249No Hit
CATGTACTCTGCGTTGATACCACTG132430.4624401390917135No Hit
GGTAAGCAGTGGTATCAACGCAGAG117580.41058454696370666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA105890.3697635454838144No Hit
GTATCAACGCAGAGTACATGGGAAG88220.30806062879008506No Hit
ACTCTGCGTTGATACCACTGCTTAC86620.3024734942847106No Hit
GTACATGGGAAGCAGTGGTAAGCAG82500.28808662293337134No Hit
CCATGTACTCTGCGTTGATACCACT79710.27834405713962457No Hit
GTACTCTGCGTTGATACCACTGCTT78250.2732457969034704No Hit
GTGGTATCAACGCAGAGTACATGGG75000.2618969299394285No Hit
GTACATAAGCAGTGGTATCAACGCA73900.2580557749669835No Hit
GCAGTGGTATCAACGCAGAGTACAT69260.24185308490139754No Hit
GGTATCAACGCAGAGTACATGGGAA67640.2361961112147059No Hit
GCTTACCACTGCTTCCCATGTACTC67150.234485051272435No Hit
GCGTTGATACCACTGCTTACCACTG62570.21849187875080056No Hit
GTACATGGGAAGCAGTGGTATCAAC60240.21035561412734893No Hit
TATCAACGCAGAGTACATGGGAAGC57850.20200983195994582No Hit
GCTTACCATGTACTCTGCGTTGATA55900.1952005117815207No Hit
GAGTACATGGTAAGCAGTGGTATCA55770.19474655710295902No Hit
GCGTTGATACCACTGCTTACCATGT54060.18877530710034005No Hit
AAGCAGTGGTATCAACGCAGAGTAC51530.17994065066371667No Hit
GTACATGATAAGCAGTGGTATCAAC47840.16705532171069679No Hit
GCTTATGTACTCTGCGTTGATACCA46590.16269037287837296No Hit
CTGCTTACCATGTACTCTGCGTTGA46120.16104915211741921No Hit
ATCATGTACTCTGCGTTGATACCAC46100.16097931293610204No Hit
CAGTGGTATCAACGCAGAGTACATG41570.14516073836776056No Hit
GTATCAACGCAGAGTACATGGTAAG41460.14477662287051607No Hit
ATGGTAAGCAGTGGTATCAACGCAG40360.1409354678980711No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA40270.14062119158214378No Hit
ACGCAGAGTACATGGGAAGCAGTGG37920.13241508777737504No Hit
ACGCAGAGTACATGGTAAGCAGTGG34110.11911072373645207No Hit
GAGTACATGGGAAGCAGTGGTAAGC34080.1190059649644763No Hit
TATCAACGCAGAGTACATGGTAAGC33780.1179583772447186No Hit
GTGATAAGCAGTGGTATCAACGCAG32950.11506005122005557No Hit
GTATCAACGCAGAGTACTTTTTTTT32540.11362834800305337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT751.72804E-1017.7301244
TAGTACG1100.015.5469852
AGTACGG1800.013.1918253
TACGGTC802.005545E-613.0599075
CGTCTAG604.0766582E-412.67300519
ACCGTTC550.00307530512.0878758
ACGGTCT752.0788086E-411.3977376
CTAGACC855.336092E-511.1744474
TACCGCA855.337991E-511.1740577
GTCCTAG1553.0559022E-1011.0480111
TAGACAG2750.010.3615785
GTATTAA3500.010.3290141
GTATAGA2700.010.2182741
ATACGGT750.002653102910.1313213
TCTAGAC1606.199116E-910.0917463
GTAGCAC1151.02953345E-59.9110763
ACATAAG16600.09.7269843
TTATACT2850.09.6649164
CATAAGC16650.09.6408974
CTAGTAC1502.595989E-79.5135651
Produced by FastQC (version 0.11.5)