FastQCFastQC Report
Wed 25 May 2016
SRR1294517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863722
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC292821.022515453664846No Hit
GTGGTAAGCAGTGGTATCAACGCAG176440.6161212575801701No Hit
ACCATGTACTCTGCGTTGATACCAC175210.6118261479291635No Hit
GTACATGGTAAGCAGTGGTATCAAC159320.5563389183726632No Hit
CATGTACTCTGCGTTGATACCACTG137560.4803538890995704No Hit
GGTAAGCAGTGGTATCAACGCAGAG130560.45591017563855707No Hit
GTATCAACGCAGAGTACTTTTTTTT108780.37985530718414706No Hit
ACTCTGCGTTGATACCACTGCTTAC92720.32377444458645077No Hit
GTATCAACGCAGAGTACATGGGAAG91910.32094595774310497No Hit
CCATGTACTCTGCGTTGATACCACT85780.299540248669389No Hit
GTACATGGGAAGCAGTGGTAAGCAG84970.29671176182604314No Hit
GTACTCTGCGTTGATACCACTGCTT77350.27010303374419725No Hit
GTGGTATCAACGCAGAGTACATGGG76340.2665761550876796No Hit
GTACATAAGCAGTGGTATCAACGCA75940.26517937146133597No Hit
GCAGTGGTATCAACGCAGAGTACAT74410.25983667409057165No Hit
GGTATCAACGCAGAGTACATGGGAA71190.24859256589850554No Hit
GCTTACCACTGCTTCCCATGTACTC68270.2383960454261971No Hit
TATCAACGCAGAGTACTTTTTTTTT67810.23678974425590193No Hit
GGTATCAACGCAGAGTACTTTTTTT67160.23451997086309356No Hit
GCGTTGATACCACTGCTTACCACTG64630.2256853144264702No Hit
TATCAACGCAGAGTACATGGGAAGC62960.21985374278648556No Hit
GTACATGGGAAGCAGTGGTATCAAC61550.21493008050362433No Hit
AAGCAGTGGTATCAACGCAGAGTAC60420.2109841667592036No Hit
GAGTACATGGTAAGCAGTGGTATCA59540.20791124278124762No Hit
GCTTACCATGTACTCTGCGTTGATA57060.1992511842979172No Hit
GCGTTGATACCACTGCTTACCATGT55070.1923021857568577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA51900.1812326755180845No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51230.17889306294395893No Hit
CTGCTTACCATGTACTCTGCGTTGA48070.16785847229584436No Hit
GTACATGATAAGCAGTGGTATCAAC47720.16663628662279367No Hit
GCTTATGTACTCTGCGTTGATACCA46540.16251577492508001No Hit
ATCATGTACTCTGCGTTGATACCAC46100.16097931293610204No Hit
CAGTGGTATCAACGCAGAGTACATG44630.15584613310928924No Hit
GTATCAACGCAGAGTACATGGTAAG43080.15043359655720773No Hit
ATGGTAAGCAGTGGTATCAACGCAG42180.14729083339793456No Hit
ACGCAGAGTACATGGGAAGCAGTGG42120.14708131585398304No Hit
ACGCAGAGTACATGGTAAGCAGTGG36620.1278755409917583No Hit
GTGATAAGCAGTGGTATCAACGCAG36600.1278057018104411No Hit
TATCAACGCAGAGTACATGGTAAGC36590.12777078221978252No Hit
GAGTACATGGGAAGCAGTGGTAAGC35280.12319631584350715No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35240.12305663748087278No Hit
GAGTACTTTTTTTTTTTTTTTTTTT32560.11369818718437055No Hit
GGTATCAACGCAGAGTACATGGTAA29360.1025239181736216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG707.251394E-613.5743724
ACGTCGC500.001497634913.3028866
TAATACG655.4445252E-513.15674
ATCGACC802.871712E-511.8736368
TCGACCG907.4761483E-611.6097779
CCGTATA752.070466E-411.4026732
TATCCCG855.3154487E-511.17875
GTTAGAC750.002643973810.1355323
CGCCTAA750.002651645810.13199214
ACGGGTC856.5844716E-410.060835
GACCCGT951.6413249E-410.002176
TAGACAG3350.09.6437145
GTATTAC2201.6370905E-119.5072091
GGTATAA3500.09.5072091
ACGCTGT800.0045093359.5020616
CAATACT1804.1709427E-99.5020614
TATTCCG1002.7511403E-49.5018965
TGGCGTA800.00450998459.5018955
AAATTCG1002.7622606E-49.49807917
GTATTAG2151.05501385E-109.2861111
Produced by FastQC (version 0.11.5)