FastQCFastQC Report
Wed 25 May 2016
SRR1294516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499583
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC106490.42603106198113844No Hit
ACCATGTACTCTGCGTTGATACCAC71220.28492752591132203No Hit
GTACATGGTAAGCAGTGGTATCAAC66630.2665644629524204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA61840.2474012665312574No Hit
GTGGTAAGCAGTGGTATCAACGCAG59500.23803970502279778No Hit
CATGTACTCTGCGTTGATACCACTG54240.21699619496532022No Hit
GTGGTATCAACGCAGAGTACATGGG44320.17730957523714955No Hit
GGTAAGCAGTGGTATCAACGCAGAG42430.16974831401877832No Hit
GTATCAACGCAGAGTACATGGGAAG36610.14646443026696854No Hit
CCATGTACTCTGCGTTGATACCACT32980.1319420079269222No Hit
ACTCTGCGTTGATACCACTGCTTAC30700.12282048645714104No Hit
GTACATAAGCAGTGGTATCAACGCA29650.11861978578026815No Hit
GGTATCAACGCAGAGTACATGGGAA28250.11301885154443761No Hit
GCAGTGGTATCAACGCAGAGTACAT27440.1097783110222785No Hit
GTACATGGGAAGCAGTGGTAAGCAG26810.10725789061615477No Hit
GCTTACCACTGCTTCCCATGTACTC26300.10521755028738793No Hit
GTACTCTGCGTTGATACCACTGCTT25810.10325722330484725No Hit
GAGTACATGGTAAGCAGTGGTATCA25160.10065678955249735No Hit
GCTTACCATGTACTCTGCGTTGATA25050.10021671614825352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA402.7613153E-416.6255072
TACCGTC602.574091E-514.2472987
CTAGTAC1052.0008883E-812.6642663
CGTCCGC604.10428E-412.66274510
TGAACCG550.003073704412.0886165
TAGGACC1553.1468517E-1011.0306084
GTCCTAG1752.3101165E-1010.3282451
CTAGGAC1951.4551915E-1110.228833
GTCTTAT2700.010.2175131
TAGACAG3050.09.9653245
GTATAGG2450.09.7069981
GGACTGT2850.09.6648346
GCGAAAA900.00110031549.5128571
TTAGGAC1606.6585926E-89.4981993
ATACCGT1002.7615955E-49.4981996
ACCGCTT1106.8593574E-59.4974398
GTCCTAC1752.6129783E-89.2410621
GACAGTC1752.6793714E-89.2268227
CTCGCTC1451.711438E-69.1706753
TTAGCAC2800.09.15897753
Produced by FastQC (version 0.11.5)