FastQCFastQC Report
Wed 25 May 2016
SRR1294516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499583
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC111530.44619442523012837No Hit
GTATCAACGCAGAGTACTTTTTTTT81920.32773466614231256No Hit
ACCATGTACTCTGCGTTGATACCAC73320.2933289272650678No Hit
GTACATGGTAAGCAGTGGTATCAAC71900.28764797968301115No Hit
GTGGTAAGCAGTGGTATCAACGCAG70790.2832072389674598No Hit
GGTATCAACGCAGAGTACTTTTTTT58850.2354392712704479No Hit
CATGTACTCTGCGTTGATACCACTG54990.21999669544880085No Hit
TATCAACGCAGAGTACTTTTTTTTT50820.20331391276064847No Hit
GGTAAGCAGTGGTATCAACGCAGAG48150.1926321310394574No Hit
GTGGTATCAACGCAGAGTACATGGG46600.1864310967069307No Hit
GTATCAACGCAGAGTACATGGGAAG39430.15774631208485576No Hit
CCATGTACTCTGCGTTGATACCACT35050.1402233892613288No Hit
ACTCTGCGTTGATACCACTGCTTAC31850.12742125386514472No Hit
GTACATAAGCAGTGGTATCAACGCA30990.12398067997742024No Hit
GGTATCAACGCAGAGTACATGGGAA30730.12294050647648028No Hit
GCAGTGGTATCAACGCAGAGTACAT30170.12070013278214806No Hit
GTACATGGGAAGCAGTGGTAAGCAG29830.11933990589630351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA27500.11001835106095696No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27420.10969829767605237No Hit
GAGTACATGGTAAGCAGTGGTATCA27250.10901818423313007No Hit
GCTTACCACTGCTTCCCATGTACTC26780.10713787059681555No Hit
GCTTACCATGTACTCTGCGTTGATA26580.10633773713455404No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26360.10545759032606639No Hit
GTACTCTGCGTTGATACCACTGCTT25860.10345725667041263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC701.0931521E-412.21220211
ACACCGT803.7637146E-410.6901676
CCTATAC1909.094947E-1210.5026213
TCTGTCG1201.5263922E-610.2907348
CTAACAC2250.010.1358633
TTAGGGT1856.4574124E-109.7591924
GTATAGA3000.09.5061771
TGGACCG1106.8205074E-59.5023725
CTGTCGC1701.6681952E-89.499149
TGTGCGA800.0045219419.49875910
AGGACTG3750.09.3756725
CAGGACT3450.09.3646564
GTCTAAA2151.05501385E-109.2851021
ATAGGAC1951.6752892E-99.2587213
TGTCGCC1451.7100683E-69.17121610
TAGGACA2700.09.1504334
ATAAGAC2501.8189894E-129.1222773
GTCTATG2106.6029315E-109.0535011
CTAGTAC1054.4826852E-49.0498773
TCTATAC2950.09.01923
Produced by FastQC (version 0.11.5)