FastQCFastQC Report
Wed 25 May 2016
SRR1294515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889211
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC124320.43029048414947885No Hit
ACCATGTACTCTGCGTTGATACCAC92350.31963743734881256No Hit
GTACATGGTAAGCAGTGGTATCAAC89430.3095308719231652No Hit
GTGGTAAGCAGTGGTATCAACGCAG69190.23947714445223972No Hit
CATGTACTCTGCGTTGATACCACTG63900.2211676474996115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA57410.19870476749534732No Hit
GGTAAGCAGTGGTATCAACGCAGAG57060.19749336410528687No Hit
GTGGTATCAACGCAGAGTACATGGG49110.16997720138819905No Hit
ATTCCATTCCATTCCATTCCATTCC44930.155509583758334No Hit
CCATGTACTCTGCGTTGATACCACT44300.15332905765622518No Hit
GTATCAACGCAGAGTACATGGGAAG42580.14737587528221371No Hit
ACTCTGCGTTGATACCACTGCTTAC39750.13758081358543908No Hit
GAATGGAATGGAATGGAATGGAATG37140.128547205448131No Hit
GGTATCAACGCAGAGTACATGGGAA34950.12096728137889548No Hit
GAGTACATGGTAAGCAGTGGTATCA33990.11764457493758677No Hit
GCAGTGGTATCAACGCAGAGTACAT33550.1161216678186536No Hit
GTACATGGGAAGCAGTGGTAAGCAG33330.11536021425918702No Hit
GCTTACCATGTACTCTGCGTTGATA33120.11463337222515073No Hit
GTACTCTGCGTTGATACCACTGCTT32480.1124182345976116No Hit
GTACATAAGCAGTGGTATCAACGCA30830.10670733290161225No Hit
GTATCAACGCAGAGTACTTTTTTTT29520.10217322307024305No Hit
GCTTACCACTGCTTCCCATGTACTC29370.10165405018878856No Hit
GCGTTGATACCACTGCTTACCATGT29260.10127332340905527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG600.005888063511.0807175
CTGTCGC1053.4795175E-610.8543929
GGACCGT909.5417745E-510.5532476
GTTCTAG2350.010.5245561
ACACCGA750.002653557610.1311186
GCGAAAA856.516828E-410.0721431
AGCACCG1054.1181924E-59.9500325
GACCGTG1151.0297899E-59.9108757
TAAGACT2750.09.3252334
TAGGACA2201.6916601E-109.0661994
GCTTATG6850.09.0265361
CAACGTT950.0018289438.99756411
CACGGTA850.0074513418.9392214
AGGGCGG850.0074545168.93875710
GTATAGG3300.08.936061
GTCTTAG2451.6370905E-118.9301781
TCTAGTA1606.737955E-78.9069242
GCTCTAG1401.0676995E-58.833111
TACACGG1401.0846659E-58.8220962
CTAGGAC1951.6572812E-88.7670093
Produced by FastQC (version 0.11.5)