FastQCFastQC Report
Wed 25 May 2016
SRR1294515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889211
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC133030.46043712279926946No Hit
ACCATGTACTCTGCGTTGATACCAC97010.33576640819933196No Hit
GTACATGGTAAGCAGTGGTATCAAC94890.3284287648081085No Hit
GTATCAACGCAGAGTACTTTTTTTT92940.3216795173492002No Hit
GTGGTAAGCAGTGGTATCAACGCAG78970.2733272163230723No Hit
CATGTACTCTGCGTTGATACCACTG65910.2281245641111016No Hit
GGTAAGCAGTGGTATCAACGCAGAG64410.22293283529655675No Hit
GGTATCAACGCAGAGTACTTTTTTT59160.20476178444564969No Hit
TATCAACGCAGAGTACTTTTTTTTT53210.1841679268146217No Hit
GTGGTATCAACGCAGAGTACATGGG52530.18181434308536135No Hit
CCATGTACTCTGCGTTGATACCACT47400.16405863053961792No Hit
ATTCCATTCCATTCCATTCCATTCC46190.15987063596255172No Hit
GTATCAACGCAGAGTACATGGGAAG46130.15966296680996994No Hit
ACTCTGCGTTGATACCACTGCTTAC41470.14353399595945054No Hit
GAATGGAATGGAATGGAATGGAATG37570.13003550104163386No Hit
GAGTACATGGTAAGCAGTGGTATCA36830.12747424815979172No Hit
GGTATCAACGCAGAGTACATGGGAA36560.12653973697317364No Hit
GCAGTGGTATCAACGCAGAGTACAT35450.12269785765041043No Hit
GTACATGGGAAGCAGTGGTAAGCAG35000.12114033900604698No Hit
GTACTCTGCGTTGATACCACTGCTT33870.11722923663242317No Hit
GCTTACCATGTACTCTGCGTTGATA33430.11570632951349001No Hit
GTACATAAGCAGTGGTATCAACGCA31610.10940703188517557No Hit
GCGTTGATACCACTGCTTACCATGT31130.10774567866452121No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30240.10466525290122458No Hit
AAGCAGTGGTATCAACGCAGAGTAC29320.10148099256163706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA29080.10065031595130988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGA658.01237E-411.6948646
GACTGCG658.026345E-411.6924367
TGTACCG600.00586892811.0855665
CGAAATG1502.1427695E-910.76454819
TAGACTG2950.010.307165
TGGACCG750.002644328910.1353745
TGCACCG750.002644328910.1353745
CCAGTAC3300.010.0777863
AGAACGG951.6415938E-410.0020145
CTAGACT1651.0197255E-89.789854
TATTCCG1304.2305655E-69.5019145
AACACGG1201.697394E-59.5019135
CCGTCTG900.0011148949.4992819
CCGAATT1106.849701E-59.49878914
CACGATC800.0045247699.49813117
ACGAAAT1651.0697477E-79.21030918
CTAATAC3450.09.0887873
GTACTAG2201.6552804E-109.0740931
GGAACGA2002.6666385E-99.02338115
TTAGACT2356.548362E-118.8954094
Produced by FastQC (version 0.11.5)