FastQCFastQC Report
Wed 25 May 2016
SRR1294514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156055
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC165670.768394127237014No Hit
ACCATGTACTCTGCGTTGATACCAC133160.6176094765671561No Hit
GTACATGGTAAGCAGTGGTATCAAC128000.5936768774451486No Hit
CATGTACTCTGCGTTGATACCACTG92720.4300446880993296No Hit
GTGGTAAGCAGTGGTATCAACGCAG82190.381205488728256No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA77250.3582932717393573No Hit
GGTAAGCAGTGGTATCAACGCAGAG72090.33436067261734975No Hit
CCATGTACTCTGCGTTGATACCACT65600.3042593996906387No Hit
GTATCAACGCAGAGTACATGGGAAG58860.2729986016126676No Hit
GTGGTATCAACGCAGAGTACATGGG52790.24484533094007344No Hit
ACTCTGCGTTGATACCACTGCTTAC52280.24247989963150288No Hit
GAGTACATGGTAAGCAGTGGTATCA49160.2280090257437774No Hit
GGTATCAACGCAGAGTACATGGGAA48240.22374197318714042No Hit
GTACTCTGCGTTGATACCACTGCTT47310.219428539624453No Hit
GTATCAACGCAGAGTACTTTTTTTT47210.21896472956394897No Hit
GCAGTGGTATCAACGCAGAGTACAT47150.21868644352764657No Hit
GCTTACCATGTACTCTGCGTTGATA45230.20978129036596932No Hit
GTACATAAGCAGTGGTATCAACGCA43270.2006906131800905No Hit
ATTCCATTCCATTCCATTCCATTCC43110.19994851708328407No Hit
GTACATGATAAGCAGTGGTATCAAC42970.1992991829985784No Hit
GCGTTGATACCACTGCTTACCATGT42310.1962380365992519No Hit
ATCATGTACTCTGCGTTGATACCAC38720.17958725542715745No Hit
GTACATGGGAAGCAGTGGTATCAAC37050.1718416274167403No Hit
CTGCTTACCATGTACTCTGCGTTGA36620.169847244156573No Hit
GAATGGAATGGAATGGAATGGAATG36450.16905876705371617No Hit
GTACATGGGAAGCAGTGGTAAGCAG35760.16585847763623843No Hit
TATCAACGCAGAGTACATGGGAAGC35380.16409599940632313No Hit
GTATCAACGCAGAGTACATGGTAAG34720.16103485300699658No Hit
GTGATAAGCAGTGGTATCAACGCAG33620.15593294234145233No Hit
AAGCAGTGGTATCAACGCAGAGTAC32950.15282541493607538No Hit
GCGTTGATACCACTGCTTACCACTG32530.15087741268195848No Hit
GCTTACCACTGCTTCCCATGTACTC31750.1472596942100271No Hit
GGTATCAACGCAGAGTACTTTTTTT28010.12991319794717668No Hit
CAGTGGTATCAACGCAGAGTACATG27830.1290783398382694No Hit
ATGGTAAGCAGTGGTATCAACGCAG27290.12657376551154773No Hit
TATCAACGCAGAGTACTTTTTTTTT27010.12527509734213646No Hit
GCTTATGTACTCTGCGTTGATACCA26640.12355900011827156No Hit
ACGCAGAGTACATGGTAAGCAGTGG26630.12351261911222117No Hit
TATCAACGCAGAGTACATGGTAAGC26510.12295604703961634No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA26060.12086890176734824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGGG604.1016506E-412.66355912
ACTGTCC1604.5474735E-1111.2776978
ACTCGGA600.005849136511.09038918
GCTTATG6700.010.9327291
TGCGTTA700.001494865210.85447912
GTCCTAC909.38808E-510.5698811
GCTATAC750.00265163810.1317863
ACTAGAC1502.451452E-810.1317863
GGAACGA856.605816E-410.05705515
ACCGCTT856.614273E-410.05565558
TTATACT3500.010.0410914
TATACTG3600.010.02508455
GTGTAGG1252.52654E-69.8934091
GTATTAG3500.09.784691
GTCTTAG1201.6737184E-59.5128941
CACCGCT1002.7633933E-49.4974487
TCTGTCG2401.8189894E-129.4970078
TGCCCGC800.00453013149.49656710
GGTGCCC1651.0710028E-79.209228
GAGTGTC1054.505323E-49.0451897
Produced by FastQC (version 0.11.5)