FastQCFastQC Report
Wed 25 May 2016
SRR1294514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156055
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC175430.8136619891422064No Hit
GTATCAACGCAGAGTACTTTTTTTT160330.7436266700060992No Hit
ACCATGTACTCTGCGTTGATACCAC139780.6483137025725225No Hit
GTACATGGTAAGCAGTGGTATCAAC135790.629807681158412No Hit
CATGTACTCTGCGTTGATACCACTG95190.44150079659377894No Hit
GTGGTAAGCAGTGGTATCAACGCAG92620.42958087803882555No Hit
GGTATCAACGCAGAGTACTTTTTTT91380.4238296332885757No Hit
TATCAACGCAGAGTACTTTTTTTTT90580.4201191528045435No Hit
GGTAAGCAGTGGTATCAACGCAGAG79430.36840433105834497No Hit
CCATGTACTCTGCGTTGATACCACT69580.3227190400986988No Hit
GTATCAACGCAGAGTACATGGGAAG63020.2922931001296349No Hit
GTGGTATCAACGCAGAGTACATGGG57750.26785030994107295No Hit
ACTCTGCGTTGATACCACTGCTTAC54150.25115314776292813No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53080.2461903801155351No Hit
GAGTACATGGTAAGCAGTGGTATCA51930.24085656441973882No Hit
GCAGTGGTATCAACGCAGAGTACAT51350.2381664660688155No Hit
GGTATCAACGCAGAGTACATGGGAA50600.23468789061503534No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47860.22197949495722513No Hit
GTACTCTGCGTTGATACCACTGCTT47390.2197995876728562No Hit
GCTTACCATGTACTCTGCGTTGATA45120.2092710992994149No Hit
GTACATAAGCAGTGGTATCAACGCA44640.2070448110089956No Hit
ATTCCATTCCATTCCATTCCATTCC43490.20171099531319936No Hit
GCGTTGATACCACTGCTTACCATGT43340.2010152802224433No Hit
GTACATGATAAGCAGTGGTATCAAC42940.19916003998042722No Hit
ATCATGTACTCTGCGTTGATACCAC40300.18691545438312102No Hit
GTACATGGGAAGCAGTGGTAAGCAG39870.18492107112295372No Hit
GTGATAAGCAGTGGTATCAACGCAG39690.18408621301404648No Hit
GTACATGGGAAGCAGTGGTATCAAC39110.18139611466312316No Hit
AAGCAGTGGTATCAACGCAGAGTAC38980.18079316158446793No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38730.17963363643320787No Hit
CTGCTTACCATGTACTCTGCGTTGA37620.17448534476161323No Hit
GAATGGAATGGAATGGAATGGAATG37020.1717024843985891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA36910.17119229333203467No Hit
TATCAACGCAGAGTACATGGGAAGC36840.17086762628968186No Hit
GTATCAACGCAGAGTACATGGTAAG35680.1654874295878352No Hit
GCGTTGATACCACTGCTTACCACTG32410.15032084060935366No Hit
CAGTGGTATCAACGCAGAGTACATG32410.15032084060935366No Hit
GCTTACCACTGCTTCCCATGTACTC31460.14591464503456544No Hit
ATGGTAAGCAGTGGTATCAACGCAG30580.14183311650213004No Hit
ACGCAGAGTACATGGTAAGCAGTGG28180.13070167505003352No Hit
TATCAACGCAGAGTACATGGTAAGC27890.12935662587457186No Hit
GCTTATGTACTCTGCGTTGATACCA27370.12694481355995094No Hit
ACGCAGAGTACATGGGAAGCAGTGG22310.1034760244984474No Hit
GATAAGCAGTGGTATCAACGCAGAG22310.1034760244984474No Hit
TACCACTGCTTACCATGTACTCTGC22060.10231649934718734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC1600.012.4697863
CCGTACC907.4688687E-611.6104589
TCCGTAC951.3616078E-510.9993828
TCGATGA700.001492796210.85628113
GTCCAAC856.5620634E-410.0643271
GGCGTGC856.5985543E-410.0582598
TTCCGTA1151.026849E-59.9129477
ACTGACC1151.0274152E-59.9124878
TATTCCG1356.435421E-79.8528995
ATAGGAC1356.437458E-79.852673
CTAGACT2401.8189894E-129.5010094
TCCCGGG2303.6379788E-129.49902615
CCGGGTT2303.6379788E-129.49880617
GTATAAT2950.09.3440931
CATAAGC10500.09.3200374
CTAATAC2451.8189894E-129.3068953
CGGGTTC2357.2759576E-129.29670318
CTGACCA1856.704795E-99.2427249
CCAGGAC1651.0644544E-79.2128863
CTAGTAG1356.8412737E-69.1489083
Produced by FastQC (version 0.11.5)