FastQCFastQC Report
Wed 25 May 2016
SRR1294513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954556
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC71530.3659654673491064No Hit
ACCATGTACTCTGCGTTGATACCAC63970.3272866062676127No Hit
GTACATGGTAAGCAGTGGTATCAAC62270.31858897877574244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47490.24297078211112907No Hit
CATGTACTCTGCGTTGATACCACTG41080.21017561021531234No Hit
GTGGTAAGCAGTGGTATCAACGCAG39350.20132449517946788No Hit
GTGGTATCAACGCAGAGTACATGGG36100.1846966779155982No Hit
GGTAAGCAGTGGTATCAACGCAGAG33990.17390138732274746No Hit
CCATGTACTCTGCGTTGATACCACT28120.14386899121846597No Hit
GTACATAAGCAGTGGTATCAACGCA27090.13859925220868577No Hit
ACTCTGCGTTGATACCACTGCTTAC24570.12570629851485451No Hit
GAGTACATGGTAAGCAGTGGTATCA24000.12279003517934509No Hit
GTATCAACGCAGAGTACATGGGAAG23200.11869703400670024No Hit
GCTTACCATGTACTCTGCGTTGATA22860.11695750850832617No Hit
GTATCAACGCAGAGTACTTTTTTTT21540.1102040565734622No Hit
GCAGTGGTATCAACGCAGAGTACAT20930.10708314317932054No Hit
GCGTTGATACCACTGCTTACCATGT19860.10160875411090804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC508.7330096E-515.1964783
AATACGC400.0052905614.2456055
CGTAGTA604.0920253E-412.6669742
TCGTGGG1950.011.20224612
AGTTCGA600.005843125711.09183618
ACTGACG600.00589008211.0799168
GGGCCGC1201.2811688E-711.0799166
TATAACG700.001491398110.8574062
CTACGAC700.001494585810.8546283
GTCTCGT803.7814793E-410.6844789
CTCGTGG1803.6379788E-1110.552311
TATAGGG1502.444176E-810.1335792
TAGACTA1054.1196152E-59.9493115
TCTCGTG1054.1219322E-59.94880310
CTCGGTC1151.030342E-59.9099865
CCTCGGT1252.5787267E-69.8774594
TTAGAAC1651.02591E-89.7856113
GTCCTAG2054.0017767E-119.7456641
GTCCTGG4250.09.6255491
TAACACT2303.6379788E-129.4975564
Produced by FastQC (version 0.11.5)