FastQCFastQC Report
Wed 25 May 2016
SRR1294513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954556
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC75470.38612349812438224No Hit
GTATCAACGCAGAGTACTTTTTTTT73440.37573750764879593No Hit
ACCATGTACTCTGCGTTGATACCAC67270.34417023610477265No Hit
GTACATGGTAAGCAGTGGTATCAAC66790.34171443540118573No Hit
GGTATCAACGCAGAGTACTTTTTTT50420.2579613989059408No Hit
GTGGTAAGCAGTGGTATCAACGCAG46160.23616616766160703No Hit
TATCAACGCAGAGTACTTTTTTTTT43780.22398948917298867No Hit
CATGTACTCTGCGTTGATACCACTG41670.21319419858013788No Hit
GGTAAGCAGTGGTATCAACGCAGAG38220.19554313102310705No Hit
GTGGTATCAACGCAGAGTACATGGG37170.19017106698401068No Hit
CCATGTACTCTGCGTTGATACCACT29700.15195266853443953No Hit
GTACATAAGCAGTGGTATCAACGCA28960.14816664244974306No Hit
ACTCTGCGTTGATACCACTGCTTAC26920.13772948945949873No Hit
GAGTACATGGTAAGCAGTGGTATCA26190.13399462588946032No Hit
GTATCAACGCAGAGTACATGGGAAG24530.12550164845622228No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23570.1205900470490485No Hit
GCAGTGGTATCAACGCAGAGTACAT23350.11946447172657115No Hit
GCTTACCATGTACTCTGCGTTGATA23190.11864587149204217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA22340.11429705774610704No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21640.11071568172004281No Hit
GCGTTGATACCACTGCTTACCATGT20360.10416687984381107No Hit
GTACATGATAAGCAGTGGTATCAAC20130.1029901420066757No Hit
GGTATCAACGCAGAGTACATGGGAA20120.10293897949201762No Hit
GTACTCTGCGTTGATACCACTGCTT19580.10017620370048237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA1302.6084308E-911.6946384
CTAATAC1751.8189894E-1211.4022723
GGCTACG700.001482240710.8654251
CCAGTAC2151.8189894E-1210.1648173
TATACTG3950.010.103285
GTATTAC1803.8926373E-1010.0354271
TTACACT2850.010.0019934
GTATAGC1054.071423E-59.9599721
ATTACCC1252.5637255E-69.88196853
GTATTAA2950.09.6683861
GTCTAGA1201.685269E-59.5072461
CCTACAC2002.6375346E-109.5018943
TCTAGAC1304.228081E-69.5018943
TAGACTC1201.6965412E-59.5018935
TCTACAC1901.0477379E-99.5018933
GTATTGG1856.6083885E-99.2502941
TAGCCTG1752.6606358E-89.2304125
GTATACC1451.6886552E-69.179411
ATACTGT4350.09.1744786
GTCCTAA1356.7813708E-69.1551261
Produced by FastQC (version 0.11.5)