FastQCFastQC Report
Wed 25 May 2016
SRR1294512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429588
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC178480.7346101478933876No Hit
ACCATGTACTCTGCGTTGATACCAC161820.6660388510315329No Hit
GTACATGGTAAGCAGTGGTATCAAC153810.6330702983386484No Hit
GTGGTAAGCAGTGGTATCAACGCAG100070.4118805328310808No Hit
CATGTACTCTGCGTTGATACCACTG95870.39459365126926865No Hit
GGTAAGCAGTGGTATCAACGCAGAG85500.3519115175083183No Hit
GTGGTATCAACGCAGAGTACATGGG75890.3123574861252196No Hit
CCATGTACTCTGCGTTGATACCACT72330.2977047960394931No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA70010.28815585193868265No Hit
GTATCAACGCAGAGTACATGGGAAG64500.26547710969925764No Hit
ACTCTGCGTTGATACCACTGCTTAC60060.2472024063339134No Hit
GAGTACATGGTAAGCAGTGGTATCA57890.23827085086031047No Hit
GGTATCAACGCAGAGTACATGGGAA56210.23135609823558562No Hit
GCTTACCATGTACTCTGCGTTGATA54430.22402975319272242No Hit
GCAGTGGTATCAACGCAGAGTACAT53070.21843209630604035No Hit
GTACTCTGCGTTGATACCACTGCTT52450.2158802233135824No Hit
GTATCAACGCAGAGTACTTTTTTTT52150.21464544605916724No Hit
GCGTTGATACCACTGCTTACCATGT47120.19394234742680652No Hit
GTACATGGGAAGCAGTGGTAAGCAG45430.18698643556026784No Hit
CTGCTTACCATGTACTCTGCGTTGA43220.17789024311940954No Hit
GTATCAACGCAGAGTACATGGTAAG42510.17496793695062704No Hit
GTACATGGGAAGCAGTGGTATCAAC41570.1710989682201262No Hit
GCTTACCACTGCTTCCCATGTACTC40280.16578942602614105No Hit
GCGTTGATACCACTGCTTACCACTG38310.1576810553888149No Hit
TATCAACGCAGAGTACATGGGAAGC37320.1536062904492449No Hit
AAGCAGTGGTATCAACGCAGAGTAC36080.14850254446432892No Hit
GTACATGATAAGCAGTGGTATCAAC35910.1478028373534937No Hit
CAGTGGTATCAACGCAGAGTACATG33540.13804809704361398No Hit
GGTATCAACGCAGAGTACTTTTTTT33170.13652520509650196No Hit
ATCATGTACTCTGCGTTGATACCAC33120.13631940888743277No Hit
ATGGTAAGCAGTGGTATCAACGCAG32870.1352904278420868No Hit
TATCAACGCAGAGTACATGGTAAGC31920.13138029986977215No Hit
ACGCAGAGTACATGGTAAGCAGTGG30980.12751133113927135No Hit
TATCAACGCAGAGTACTTTTTTTTT30820.12685278327024993No Hit
GTGATAAGCAGTGGTATCAACGCAG29140.11993803064552508No Hit
ATTCCATTCCATTCCATTCCATTCC28530.11742731689488094No Hit
GTACATAAGCAGTGGTATCAACGCA27530.1133113927134971No Hit
GGTATCAACGCAGAGTACATGGTAA25700.10577925146156468No Hit
ACGCAGAGTACATGGGAAGCAGTGG24990.10285694529278215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGC402.7566735E-416.6291452
CGACGCA456.774725E-414.7747685
TCGACGC400.005285210414.2479774
AGCGTAG550.003031588612.1108841
CTAGACT1950.011.2035384
AGCGGAG700.001471521410.8750791
GCGGCCT856.613159E-410.05594613
TTAGACT1151.0286352E-59.9116364
TTTAGAC1554.074718E-89.805063
CCTAAAC1651.0251824E-89.786493
TAGACTG2054.1836756E-119.7297255
GTTCTAA2303.6379788E-129.5156941
GTATCGA900.0010972969.5156941
GTAAGAC1901.0550139E-99.4986523
TATGGCT1804.1945896E-99.4986514
TAGACAG2106.730261E-119.4980655
CTGTCGC2304.1836756E-119.084179
GTATTAG2106.475602E-109.0625661
TTATACT3250.09.0602524
GTCCCAC1701.6434933E-78.9559471
Produced by FastQC (version 0.11.5)