FastQCFastQC Report
Wed 25 May 2016
SRR1294512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429588
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC192190.7910394684201602No Hit
GTATCAACGCAGAGTACTTTTTTTT173990.7161296483189743No Hit
ACCATGTACTCTGCGTTGATACCAC170630.7023001430695245No Hit
GTACATGGTAAGCAGTGGTATCAAC157610.648710810227907No Hit
GGTATCAACGCAGAGTACTTTTTTT110820.4561267177809571No Hit
GTGGTAAGCAGTGGTATCAACGCAG110640.45538585142830795No Hit
TATCAACGCAGAGTACTTTTTTTTT100660.4143089280980973No Hit
CATGTACTCTGCGTTGATACCACTG99670.41023416315852723No Hit
GGTAAGCAGTGGTATCAACGCAGAG93160.3834394967377185No Hit
CCATGTACTCTGCGTTGATACCACT76290.3140038557977731No Hit
GTGGTATCAACGCAGAGTACATGGG74650.30725374014030365No Hit
GTATCAACGCAGAGTACATGGGAAG66250.2726799770166794No Hit
ACTCTGCGTTGATACCACTGCTTAC64430.2651889950065608No Hit
GAGTACATGGTAAGCAGTGGTATCA58970.24271604897620502No Hit
GCAGTGGTATCAACGCAGAGTACAT56730.23349637880990523No Hit
GGTATCAACGCAGAGTACATGGGAA55960.23032711719023968No Hit
GCTTACCATGTACTCTGCGTTGATA55390.22798104040685088No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55250.22740481102145713No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54660.2249764157544407No Hit
GTACTCTGCGTTGATACCACTGCTT54290.22345352380732866No Hit
GCGTTGATACCACTGCTTACCATGT50340.2071956232908625No Hit
GTACATGGGAAGCAGTGGTAAGCAG47800.19674117587014756No Hit
CTGCTTACCATGTACTCTGCGTTGA44320.18241775971893176No Hit
GTACATGGGAAGCAGTGGTATCAAC42780.17607923647960066No Hit
GTATCAACGCAGAGTACATGGTAAG41680.17155171988007845No Hit
AAGCAGTGGTATCAACGCAGAGTAC40940.1685059359858544No Hit
GCTTACCACTGCTTCCCATGTACTC40720.16760043266594993No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40410.16632449616972098No Hit
TATCAACGCAGAGTACATGGGAAGC39540.16274364213191703No Hit
GCGTTGATACCACTGCTTACCACTG37280.15344165348198954No Hit
CAGTGGTATCAACGCAGAGTACATG35720.14702081175903076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35600.1465269008572647No Hit
GTACATGATAAGCAGTGGTATCAAC34770.1431106837867161No Hit
TATCAACGCAGAGTACATGGTAAGC34150.14055881079425814No Hit
ACGCAGAGTACATGGTAAGCAGTGG33980.13985910368342286No Hit
ATGGTAAGCAGTGGTATCAACGCAG33540.13804809704361398No Hit
ATCATGTACTCTGCGTTGATACCAC33340.1372249122073372No Hit
GTGATAAGCAGTGGTATCAACGCAG31940.13146261835339984No Hit
ATTCCATTCCATTCCATTCCATTCC31070.1278817643155959No Hit
GTACATAAGCAGTGGTATCAACGCA27310.11240588939359267No Hit
ACGCAGAGTACATGGGAAGCAGTGG26990.11108879365554984No Hit
GGTATCAACGCAGAGTACATGGTAA25950.10680823250691063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATT500.001502275613.29729217
GAACGGC550.00307161712.08969211
GTCCTAC752.0636112E-411.4065991
GTATAGC951.3522569E-511.0063671
GGAACGA1106.063301E-610.361526515
TAAGCTC856.584437E-410.0607095
ACTCGGA856.601906E-410.0578111
CCCCTAT951.6351126E-410.0057881
CGTGTTA1054.1104162E-59.95158310
GACTGAT1252.5691243E-69.8806327
TATTCCG1002.7510893E-49.50178055
CTAGGAC1502.6423368E-79.50178053
GGGTGTC1804.1800376E-99.50060757
TAATACT2750.09.3290214
CCAGAAC2650.09.3225023
GAACCAT1752.6628186E-89.2303016
GGTTTAT2700.09.1534431
GATCTAT1252.7362661E-59.1252791
TAATACA4800.09.1058744
GTACATA12700.08.9815741
Produced by FastQC (version 0.11.5)