FastQCFastQC Report
Wed 25 May 2016
SRR1294511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163700
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC122570.5664833387253316No Hit
ACCATGTACTCTGCGTTGATACCAC92270.42644544068031615No Hit
GTACATGGTAAGCAGTGGTATCAAC92120.4257521837593012No Hit
GTGGTAAGCAGTGGTATCAACGCAG74720.34533438092156954No Hit
CATGTACTCTGCGTTGATACCACTG67260.3108564033830938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA61650.2849285945371355No Hit
GGTAAGCAGTGGTATCAACGCAGAG56210.2597864768683274No Hit
GTGGTATCAACGCAGAGTACATGGG44510.20571243702916303No Hit
CCATGTACTCTGCGTTGATACCACT42310.1955446688542774No Hit
ACTCTGCGTTGATACCACTGCTTAC39110.1807551878726256No Hit
GTATCAACGCAGAGTACATGGGAAG38350.1772426861394833No Hit
GTACATAAGCAGTGGTATCAACGCA37490.17326801312566437No Hit
GAGTACATGGTAAGCAGTGGTATCA34390.1589407034246892No Hit
GCAGTGGTATCAACGCAGAGTACAT33940.15686093266164441No Hit
GCTTACCATGTACTCTGCGTTGATA33610.15533576743541158No Hit
GTACTCTGCGTTGATACCACTGCTT33000.1525165226232842No Hit
GGTATCAACGCAGAGTACATGGGAA32360.14955862642695383No Hit
GTACATGGGAAGCAGTGGTAAGCAG30800.1423487544483986No Hit
GCGTTGATACCACTGCTTACCATGT28220.13042473540694183No Hit
GCTTACCACTGCTTCCCATGTACTC28000.12940795858945325No Hit
GTACATGATAAGCAGTGGTATCAAC27870.12880713592457366No Hit
GCGTTGATACCACTGCTTACCACTG26950.12455516014234876No Hit
ATCATGTACTCTGCGTTGATACCAC26700.1233997319406572No Hit
GTACATGGGAAGCAGTGGTATCAAC26150.12085778989693581No Hit
GCTTATGTACTCTGCGTTGATACCA24860.11489578037620742No Hit
AAGCAGTGGTATCAACGCAGAGTAC24810.11466469473586911No Hit
CTGCTTACCATGTACTCTGCGTTGA24310.11235383833248602No Hit
TATCAACGCAGAGTACATGGGAAGC24190.11179923279567407No Hit
GTATCAACGCAGAGTACTTTTTTTT23180.10713130286084023No Hit
GTATCAACGCAGAGTACATGGTAAG23100.10676156583629894No Hit
ATTCCATTCCATTCCATTCCATTCC22810.10542126912233674No Hit
CAGTGGTATCAACGCAGAGTACATG21930.1013541618523825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGT1152.5465852E-1114.0395329
TCGTGGG1800.013.71912
CTCGTGG1600.012.46553511
CCGTGTA752.0746015E-411.3999592
TCTCGTG1850.011.29412410
GTAGCAC1053.4764635E-610.8548453
GTCTAGG1451.4189936E-810.4946091
GTACATA12500.010.1955131
CTAGTAC1409.7224984E-810.1764173
GTAAGAC1606.195478E-910.0916143
GGTCTCG2550.010.0559978
TACGCAG951.6491686E-49.99718955
GGCTCGG1856.4210326E-109.76213717
CATAAGC9350.09.7519464
ACATAAG10000.09.6879493
GTACTAG1401.0426465E-69.5107391
GTCTTAC1804.101821E-99.5107391
GCTCGGG1502.6277667E-79.505239518
TAGACAG2800.09.4973325
TATTCCG900.00111675199.497335
Produced by FastQC (version 0.11.5)