FastQCFastQC Report
Wed 25 May 2016
SRR1294511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163700
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC131400.607293062809077No Hit
ACCATGTACTCTGCGTTGATACCAC97390.450108610250959No Hit
GTACATGGTAAGCAGTGGTATCAAC93180.4306511993344734No Hit
GTGGTAAGCAGTGGTATCAACGCAG81430.37634607385497065No Hit
GTATCAACGCAGAGTACTTTTTTTT78660.36354392938022834No Hit
CATGTACTCTGCGTTGATACCACTG69650.32190229699126494No Hit
GGTAAGCAGTGGTATCAACGCAGAG60030.2774414197901742No Hit
GGTATCAACGCAGAGTACTTTTTTT52330.24185423117807459No Hit
TATCAACGCAGAGTACTTTTTTTTT47000.2172205019180108No Hit
CCATGTACTCTGCGTTGATACCACT45920.21222905208670334No Hit
GTGGTATCAACGCAGAGTACATGGG45280.20927115589037298No Hit
ACTCTGCGTTGATACCACTGCTTAC42370.19582197162268336No Hit
GTATCAACGCAGAGTACATGGGAAG41260.1906918704071729No Hit
GTACATAAGCAGTGGTATCAACGCA38030.1757637380413181No Hit
GCAGTGGTATCAACGCAGAGTACAT36470.16855386606276287No Hit
GAGTACATGGTAAGCAGTGGTATCA36230.167444654989139No Hit
GTACATGGGAAGCAGTGGTAAGCAG33620.15538198456347924No Hit
GCTTACCATGTACTCTGCGTTGATA33380.15427277348985535No Hit
GTACTCTGCGTTGATACCACTGCTT33060.15279382539169015No Hit
GGTATCAACGCAGAGTACATGGGAA32880.15196191708647225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA31550.14581503905347323No Hit
GCGTTGATACCACTGCTTACCATGT30130.13925220686786524No Hit
GCTTACCACTGCTTCCCATGTACTC29560.1366178305680085No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28240.13051716966307714No Hit
GTACATGATAAGCAGTGGTATCAAC28190.1302860840227388No Hit
AAGCAGTGGTATCAACGCAGAGTAC28180.13023986689467115No Hit
GTACATGGGAAGCAGTGGTATCAAC28130.13000878125433285No Hit
GCGTTGATACCACTGCTTACCACTG27110.12529463419143136No Hit
ATCATGTACTCTGCGTTGATACCAC26740.12358460045292785No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26690.12335351481258955No Hit
CTGCTTACCATGTACTCTGCGTTGA25860.1195174931829736No Hit
GCTTATGTACTCTGCGTTGATACCA25190.11642094560244028No Hit
TATCAACGCAGAGTACATGGGAAGC25040.11572768868142534No Hit
GTATCAACGCAGAGTACATGGTAAG23910.11050515320977954No Hit
ATTCCATTCCATTCCATTCCATTCC23590.10902620511161437No Hit
CAGTGGTATCAACGCAGAGTACATG23080.10666913158016361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCCG250.006026471519.0034664
AACCGTG456.76397E-414.7777427
TATTCCG903.632522E-813.7247275
AAACCGT600.005868942511.0853566
CTAGGAC1605.4205884E-1010.689453
CTAGCAC1201.5206988E-610.2935453
GTATTAG2250.010.1401051
CCGTGCA750.002650374810.1323719
CTAGTAC856.5841165E-410.0606583
TTCCGTA856.59532E-410.0587997
GACTTAC1054.0761977E-59.9590321
GTCTAGT1201.6874192E-59.5063491
GATCTAA1201.6874192E-59.5063491
GACCTAT1106.824378E-59.5017346
ACATAAG8900.09.3949733
TCAAGAC2451.8189894E-129.3078213
ATAGGAC1451.7023667E-69.1740883
CCTGTAC2900.09.1740883
GGACAGT2600.09.1362836
TAGGGTG1252.7486398E-59.1216645
Produced by FastQC (version 0.11.5)